40 results about "P-aminothiophenol" patented technology

The invention relates to a piece of surface enhanced raman detection test paper and the application thereof, which belong to the technical field of ultra-sensitive test analysis, in particular to the piece of surface enhanced raman detection test paper and the application of the paper to detection of biological or chemical probe molecules with raman signals. The surface enhanced raman detection test paper is obtained by covering a precious metal layer on the surface of paper with a natural fiber micro nano multi-grade structure through the physical vapor deposition or chemical plating technology, the paper can be filter paper, parchment paper, napkin, newspaper or printing paper and the like, the precious metal film is gold or silver and the like, and the thickness of the precious metal film is 5 nanometers to 90 nanometers. Combined action of size, period and roughness enables incident light to generate a local electric field on the surface of a multi-stage structure and enables the electric field to be enhanced, and the detection limit can reach 10-10mol/L. The test paper has the advantages of being good in flexibility, low in cost, free of environment pollution, capable of being prepared in batch and the like, thereby being capable of being used in detection of probe molecules such as rhodamine 6G, p-aminothiophenol, riboflavin or ethanol and the like.

The invention discloses a method for detecting a potassium ion by preparing a biosensor based on a G-quadruplex and a gold nanoparticle, and belongs to the field of the biosensor. A detection method comprises the following steps of: modifying the gold nanoparticle on the surface of a gold electrode by taking para-aminothiophenol (p-ATP) as a medium, fixing a DNA (Deoxyribose Nucleic Acid) marked by ferrocene (Fc) and capable of forming a G-quadruplex structure onto the surface of the gold nanoparticle by a sulfydryl self-assembly action, and taking the DNA as an aptamer to prepare an electrochemistry biosensor to detect the potassium ion. The method for detecting the potassium ion by preparing the biosensor based on the G-quadruplex and the gold nanoparticle has the advantages that the detection is simple and quick, the sensitivity is high, the real-time detection can be carried out on the potassium ion, the minimum detection concentration to the potassium ion reaches 0.1 mM, and the linear range of the detection is large. In a concentration range of 0.1-1 mM, a square wave volt-ampere peak current is in a well linear relation with the concentration of the potassium ion; and in a concentration range of 1-30 mM, the square wave volt-ampere peak current is in a well linear relation with the logarithm of the concentration of the potassium ion.

The invention relates to an aptamer based method for detecting surface enhanced raman spectroscopy of a tetracycline residue in milk. The method is as follows: a prepared gold nanoparticle/silicon dioxide nuclear shell composite nanomaterial modifying a p-aminothiophenol labelled molecule is taken as a surface enhanced raman base, and an adding standard recovery measurement mode is used to realize the detection and study of the tetracycline residue in the milk. According to the method for detecting the surface enhanced raman spectroscopy of the tetracycline in the milk, the existence of the tetracycline affects the structure of an aptamer in a detection system, the tetracycline and the aptamer are specifically identified and combined, so that the enhanced bases at different concentrations are released under the existence of the tetracycline at different concentrations, then the detection system is magnetically separated, a composite containing a magnetic microsphere, the aptamer and the tetracycline is adsorbed to the bottom, supernates containing the enhanced bases at different concentrations are subjected to raman signal measurement, and the purpose of sensitively and indirectly detecting the tetracycline residue in the milk is realized according to the characteristic peak map of the labelled molecule at different raman intensities.

The invention discloses a method for fast detecting nitrite in food through fluorescent/colorimetric double probes. The method comprises the following steps of (1) preparing a gold nanometer rod modified by p-aminothiophenol; (2) preparing gold nanometer spheres modified by 1,8-diaminonaphthalene; (3) proportionally mixing the two kinds of nanometer materials; adding the mixture into the same kind of food to be tested with different known nitrite concentrations; obtaining optical photos in a fluorescent lamp and an ultraviolet lamp; (4) dripping the two kinds of nanometer materials mixed in the step (3) into the food to be tested; performing analysis and detection at the same time by a colorimetric method and a fluorescent method. The field fast qualitative and quantitive analysis on the nitrite in the food can be realized through comparing the color in the fluorescent lamp and the ultraviolet lamp. The method has the characteristics that the use cost is low; the instrument equipment is simple; the analysis is fast; the sensitivity is high; the sample consumption is low; the application range is wide, and the like. The field fast detection on the nitrite in the food can be realized.

A preparation method and a detachment method of a detachable magnetic-microsphere-supported noble metal catalyst belong to the technical field of the recovery of magnetic-microsphere-supported noble metal catalysts and noble metal catalysts. The preparation method comprises the following steps: carbon-coated ferroferric oxide magnetic microspheres are prepared with a solvothermal method; beta-cyclodextrin is chemically bonded to MFC through epoxy chloropropane under an alkaline condition, so as to form a magnetic carrier with host-guest inclusion capability; self-assembly is performed through inclusion reaction between cyclodextrin and p-aminothiophenol; finally, the detachable magnetic-microsphere-supported noble metal catalyst is prepared through complexing between sulfydryl and amino on p-aminothiophenol and noble metal nanoparticles. The adopted instruments are simple, the preparation cost is low, the operation process is simple and convenient, the reaction process requires low-toxicity and harmless materials, the species and the contents of supported noble metals can be effectively controlled, and the preparation method can be used for industrial large-batch production of the high-quality detachable magnetic-microsphere-supported noble metal catalyst.

The invention discloses a method for forming a SERS active substrate by assembling gold nanoparticles to an electrostatic spinning polymer nanofiber membrane and aims at building the active SERS substrate for sensitive detection. The method is based on the gold nanoparticles assembled by the electrostatic spinning polycarpolaction nanofiber membrane; the method comprises the following steps: firstly, inducing the crylic acid to perform grafting modification on the hydrophobic surface of the polycarpolaction nanofiber membrane by adopting the ultraviolet light, and then soaking the polycarpolaction nanofiber membrane in a gold nanoparticle solution for promoting the gold nanoparticles to be assembled to the polycarpolaction nanofiber so as to form the SERS active substrate; a p-aminothiophenol (4-ATP) molecule is adopted as an experiment probe molecule for SERS research, and the research shows that the substrate has the high SERS response sensitivity; the method has great advantages on environment-friendly synthesis, large scale, high stability and good repeatability; the highly active SERS substrate can be used for probing medicine molecules such as 2-thiouracil.

The invention discloses a preparation method for an antistatic terylene tablecloth, and belongs to the technical field of textile materials. The preparation method comprises the following steps: carrying out continuous modification treatment on the terylene tablecloth sequentially through the mixed solution composed of Na2CO3 solution, N-(2-aminoethyl)-3-aminopropyl trimethoxy-silane and vinyl trimethoxy silane, and ammonium persulphate solution, then impregnating the terylene tablecloth in the mixed conjugated monomer solution composed of phenylamine and p-aminothiophenol, and carrying out polymerization reaction, washing and drying to prepare the antistatic terylene tablecloth under the synergistic effect of perchloric acid, 4-thiacalixarene sulfonated sodium and phenol red sodium salt. The antistatic terylene tablecloth prepared by the method disclosed by the invention has an electrical conductivity of 8.5*10<-3> to 3.6*10<-2> S.cm<-1>; the preparation method disclosed by the invention has the following characteristics that the preparation method is simple, and the product is excellent in antistatic performance, wide in applicability and the like.

The invention discloses a method for separating a genistein monomer from a daidzein monomer. The method comprises a preparation process of a gold nanotubule membrane, a modification process of the gold nanotubule membrane, a preparation process of an aluminum chelate and a membrane separation process. The method helps realize high-efficiency selective separation of the genistein monomer and the daidzein monomer by utilizing mechanism that the gold nanotubule membrane is modified with p-aminothiophenol so that the membrane is positively charged at a lower pH value, and plays electrostatic repulsion on positively charged genistein and aluminum chelate without repulsion on daidzein which can not form a chelate with the aluminum. The method has the advantages of simple operation, low energy consumption and high efficiency of separation, no pollution, high purity of the monomers separated, industrialized implementation and the like. The method is of great theoretical significance for further research on pharmacological action, mechanism, popularization and application of genistein and daidzein.

The invention discloses a rapid on-site detection method for trichlorfon and simazine in seawater. The method comprises the following steps: (1) synthesizing gold nanorods by a seeding method; (2) adding L-cysteine to a gold nanorod solution to obtain an L-cysteine -modified gold nanorod material; (3) hydrothermally synthesizing gold nanospheres; (4) adding p-aminothiophenol to gold nanospheres toobtain a p-aminothiophenol-modified gold nanosphere composite; (5) mixing the L-cysteine -modified gold nanorod material with the p-aminothiophenol-modified gold nanosphere composite to obtain a goldnanorod and gold nanosphere composite as a SERS active substrate by peptide bonding; and (6) detecting the trichlorfon and the simazine by a Raman spectrometer to achieve qualitative and quantitativeanalysis of the trichlorfon and the simazine in the seawater. The rapid on-site detection method has the characteristics such as rapid analysis, high sensitivity, small sample consumption, wide application range, simple operation and convenient carrying.

The invention belongs to the technical field of food safety and material chemistry, and relates to a method for detecting nitrite ions based on a gold-silver core-shell nano-particle SERS technology.The method comprises the steps that a gold-silver core-shell nano-particle solution is prepared; nitrite ion solutions of different concentrations are prepared; a p-aminothiophenol solution and 1-naphthylamine are added respectively, and then a gold-silver core-shell nanoparticle solution is added; a raman spectrometer is used to collect raman spectra of nitrite ions of different concentrations; astandard curve of the raman signal intensity corresponding to the nitrite ion concentration is established; a sample to be tested is selected to react with p-aminothiophenol; 1- naphthylamine is added for reaction, and then the gold-silver core-shell nanoparticle solution is added for reaction; the raman spectrometer is used to collect spectra; and the concentration of the nitrite ions of the sample to be tested is acquired according to the established standard curve. According to the invention, a non-labeling method is used to detect nitrite ions, which saves the detection cost and improvesthe detection speed and stability.

The invention discloses a fluorescence sensor and a preparation method and application thereof. The fluorescence sensor is made from a nanocomposite consisting of aminated carbon quantum dots (N-CQDs)and p-aminothiophenol modified silver nanoparticles (N-AgNPs). During preparation, the aminated carbon quantum dots are prepared firstly, then the p-aminothiophenol modified silver nanoparticles areprepared, and finally the N-CQDs/N-AgNPs nanocomposite fluorescence sensor is prepared from the aminated carbon quantum dots and the p-aminothiophenol modified silver nanoparticles. The fluorescence sensor can be used for detecting the content of dopamine (DA) in serum; the prepared fluorescence sensor can detect the dopamine content in a serum sample conveniently and simply; and the method has the advantages of being cheap, simple, high in sensitivity and selectivity, wide in linear range, short in detection time and the like and has huge application prospects in DA detection in the biological analysis or disease research process.

The invention discloses a surface enhanced Raman substrate material for detecting the water content of an organic solvent, and a preparation method and an application thereof. The preparation method comprises the following steps: (1) modifying polyvinyl alcohol gel with a p-aminothiophenol derivative 4,4'-dimercaptoazobenzene as a probe molecule; (2) preparing PVA-DMAB microspheres with uniform particle sizes and smooth surfaces in a micro-reactor; and (3) loading nano-silver particles on the surfaces of the PVA-DMAB microspheres through in-situ growth in order to prepare the PVA-DMAB-Ag Ramansubstrate material. The preparation method is simple, and the prepared Raman probe can realize the rapid, quantitative and qualitative analysis of water in the organic solvent, has the advantages ofrepeated use and low detection sensitivity, and has a great application prospect in the field of analysis and detection of a micro or trace amount of water.

The invention relates to a molecular imprinting sensor for detecting antibiotic pefloxacin and a preparation method thereof. A molecular imprinting electrochemical sensor which can specifically identify the pefloxacin is prepared based on an electroplating process. According to the molecular imprinting electrochemical sensor, a gold electrode serves as a working electrode and is modified by p-aminothiophenol, so that binding sites of the pefloxacin are increased, mixed solution of chloroauric acid, p-aminothiophenol and tetrabutylammonium perchlorate serves as electroplating solution, a molecular imprinting identification film is prepared through electroplating, and the template pefloxacin in the molecular imprinting identification film is removed by employing an electrolytic dissociation method of hydrochloric acid. The prepared molecular imprinting electrochemical sensor is stable in structure, simple in preparation method, high in sensitivity and high in specificity and can be repeatedly used. The trace detection of pefloxacin in milk is realized, the detection sensitivity is improved, and the method is low in cost and has a good application prospect.

The invention provides a method for modifying different mercaptan or thiophenol molecules on the surface of plasmon metal to regulate and control a hot carrier-driven photocatalytic reaction generatedby plasmon. In the method, mercaptan and thiophenol molecules can be chemically adsorbed on the surface of the surface plasmon metal, and the operation process is simple, convenient and rapid. Meanwhile, it has been proved that the method can regulate plasmon-driven oxidation reaction of p-aminothiophenol, reduction reaction of p-nitrothiophenol, silver corrosion reaction, decarboxylation reaction of p-mercaptobenzoic acid and the like.

The invention discloses a flexible light-transmitting metamaterial composite film and method for detecting nitrite. A single layer of metal nanoparticle array is formed on a soft light-transmitting substrate as a metamaterial layer, and a p-aminothiophenol self-assembled monomolecular film covers the single layer of metal nanoparticle array as a nitrite sensitive film; under the excitation of visible/near infrared light, surface plasmon resonance is generated on the metamaterial layer; p-aminothiophenol in the nitrite sensitive film is induced to be converted into an azobenzene compound underthe synergistic action of surface ions of the metamaterial layer and sub-salt nitrate ions; since the molecules of the azobenzene compound and the molecules of the p-aminothiophenol have a significantdifference in the aspect of Raman spectrum, the reaction is directly monitored by using the Raman spectrum, and the specific recognition and detection of nitrite ions are indirectly realized. The method utilizes the special optical properties possessed by the metamaterial and combines the surface selectively of sub-salt nitric acid to catalyze aniline so as to generate the azobenzene, thereby realizing the rapid detection of nitrite.

The invention discloses a method for rapidly detecting formaldehyde and acetaldehyde in wine on site. The method is characterized in that a paper-based microfluidic analysis device [mu]-PAD is used for extracting formaldehyde and acetaldehyde from an edible wine sample, and with p-aminothiophenol ATP as a probe molecule and silver nanoparticles Ag NPs prepared during a silver mirror reaction as asurface-enhanced Raman scattering substrate, a change relationship between the peak intensity of the p-aminothiophenol and formaldehyde or acetaldehyde content is established through a portable Ramanspectrometer, so that on-site rapid quantitative and qualitative detection of formaldehyde and acetaldehyde in the to-be-detected wine sample is achieved. Compared with the existing method for rapidlydetecting formaldehyde and acetaldehyde on site, the method provided by the invention has the characteristics of being high in selectivity, fast in analysis, high in sensitivity, low in sample amount, wide in application range, simple and convenient to operate, convenient to carry and the like. With the method, the formaldehyde detection limit is 0.0046 [mu]M and the acetaldehyde detection limitis 0.0032 [mu]M.

The invention provides a production method of a simple and high-effective Raman enhanced substrate. The production method includes the steps of: respectively preparing a porous gold thin film and a seed crystal; soaking the porous gold thin film in a SnCl2 solution and air-drying the thin film, and soaking the thin film in a water solution containing the seed crystal, silver nitrate and polyvinylpyrrolidone so as to prepare an Ag@NPG substrate under irradiation of ultraviolet light, namely, the Raman enhanced substrate, wherein the porous gold thin film is prepared through a dealloying method. The seed crystal is prepared through a step of adding an AgNO3 water solution to a sodium citrate water solution with intensive stirring and quick addition of a NaBH4 solution to prepare the seed crystal. Compared with the prior art, the substrate integrates the porous gold and silver nano particles and can be matched with a portable Raman spectrum instrument for detection of molecules such as p-aminothiophenol, crystal violet in water solution, rhodamine 6G, etc.

The invention discloses a construction method and application of a flexible surface-enhanced Raman substrate, and belongs to the technical field of surface-enhanced Raman detection. A flexible substrate takes cellulose diacetate and gold nanoparticles as raw materials, the gold nanoparticles are filtered and intercepted on a prepared cellulose diacetate film, and then p-aminothiophenol and thiram pesticides are qualitatively and quantitatively analyzed by using a dropwise adding test or filtering enrichment test method. The method for detecting pesticide residues such as thiram has the advantages of no damage, rapidness, real-time performance, high sensitivity and the like, so that an effective method is provided for rapid determination of low-concentration pesticides, and the method has practical application value.

The SERS test method uses nano semiconductor material as surface enhancement of Raman scattering base, and enhances the SERS signal up to 104 times. Wherein, this invention is fit to cadmium telluride, cadmium sulphide, ZnO, ZnS, lead sulfide, TiO2, Pb3O4 or Pb2O3, and mercaptopyridine, p-aminobenzenethiol, pyridine, sulfhydrylbenzoic acid, 1,4-2[(4-pyridyl) vinyl]-benzene, 2-2bipyridyl or 4-4bipyridyl. This invention has wide application.