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Method for fast detecting nitrite in food through fluorescent/colorimetric double probes

A nitrite and dual-probe technology, which is applied in fluorescence/phosphorescence, measuring devices, and material analysis through optical means, can solve problems such as unfavorable food on-site detection, matrix interference analyte concentration, etc., to achieve convenient promotion and use, The effect of small background interference and wide detection range

Inactive Publication Date: 2017-08-08
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods require large instruments and equipment, which is not conducive to the on-site detection of food
In addition, food often has the characteristics of complex matrix interference and low analyte concentration. Therefore, it is necessary to develop an on-site rapid detection method for nitrite in food samples to meet the needs of on-site rapid detection of nitrite in food samples.

Method used

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  • Method for fast detecting nitrite in food through fluorescent/colorimetric double probes
  • Method for fast detecting nitrite in food through fluorescent/colorimetric double probes
  • Method for fast detecting nitrite in food through fluorescent/colorimetric double probes

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Embodiment 1

[0031] (1) Preparation of gold nanorods by seed crystal method

[0032] Preparation of gold nano seed solution: at room temperature (25-28°C), prepare 9.75ml 0.1mol / L cetyl ammonium bromide aqueous solution, stir evenly until transparent, add dropwise 0.25ml 0.01mol / L tetrachloroauric acid aqueous solution , after it is uniformly dispersed in the solution, quickly add freshly prepared 0.01mol / L NaBH at 0°C 4 The solution was 0.6ml, the solution changed from light yellow to brownish yellow, stirred evenly for 3min, and stood at room temperature for 2h before use. At this time, the gold concentration was 0.25 mmol.

[0033] Preparation of gold nanorod solution: at room temperature, prepare 10ml of 0.1mol / L cetyltrimethylammonium bromide aqueous solution, then add 0.5ml of 0.01mol / L tetrachloroauric acid aqueous solution, mix well and then add 0.1ml 0.01mol / LAgNO 3 , 0.2ml 1M HCl, stir well, add 80μL 0.1mol / L ascorbic acid, the solution turns from dark yellow to colorless, add...

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Abstract

The invention discloses a method for fast detecting nitrite in food through fluorescent / colorimetric double probes. The method comprises the following steps of (1) preparing a gold nanometer rod modified by p-aminothiophenol; (2) preparing gold nanometer spheres modified by 1,8-diaminonaphthalene; (3) proportionally mixing the two kinds of nanometer materials; adding the mixture into the same kind of food to be tested with different known nitrite concentrations; obtaining optical photos in a fluorescent lamp and an ultraviolet lamp; (4) dripping the two kinds of nanometer materials mixed in the step (3) into the food to be tested; performing analysis and detection at the same time by a colorimetric method and a fluorescent method. The field fast qualitative and quantitive analysis on the nitrite in the food can be realized through comparing the color in the fluorescent lamp and the ultraviolet lamp. The method has the characteristics that the use cost is low; the instrument equipment is simple; the analysis is fast; the sensitivity is high; the sample consumption is low; the application range is wide, and the like. The field fast detection on the nitrite in the food can be realized.

Description

technical field [0001] The invention belongs to the technical field of food detection, and in particular relates to a method for rapidly detecting nitrite in food with fluorescence / colorimetric dual probes. Background technique [0002] In the traditional food industry such as the processing of meat products (such as sausages, braised meat products, etc.), nitrite is often used as a common coloring agent and flavoring agent, which can obtain ideal color and flavor, and has a certain antibacterial and antiseptic ability. However, when nitrite coexists with secondary amines, tertiary amines, phthaloamines and amino acids in an acidic environment, it can form N-nitrosamines (Nitrosamines) compounds with strong carcinogenic effects. Therefore, controlling the content of nitrite, the precursor compound of amine nitrite in food, has important theoretical and practical significance. [0003] At present, the determination of NO reported at home and abroad 2 - Most of the methods ...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N21/78
CPCG01N21/6428G01N21/643G01N21/78G01N2021/6432
Inventor 李丹邓维马亚丹段化珍
Owner SHANGHAI INST OF TECH
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