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Molecular imprinting sensor for detecting antibiotic pefloxacin and preparation method thereof

A technology of pefloxacin and molecular imprinting, applied in the direction of material electrochemical variables, etc., can solve the problems of limited application, loss of target substances, expensive instruments, etc., and achieve the effects of stable properties and structures, strong specificity, and low cost

Inactive Publication Date: 2013-03-13
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The traditional methods for detecting fluoroquinolone antibiotics mainly include liquid chromatography, gas chromatography, enzyme-linked immunosorbent assay, gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry, etc. Although the enzyme-linked immunosorbent assay has high sensitivity, the establishment of this The method requires complex processes including the preparation of antigens and antibodies, and because of its biological activity, the conditions during operation and storage are relatively strict
For chromatographic detection methods, the focus is on pre-treatment, which can easily cause the loss of target substances, and expensive instruments require professional operation, which also limits its application

Method used

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  • Molecular imprinting sensor for detecting antibiotic pefloxacin and preparation method thereof
  • Molecular imprinting sensor for detecting antibiotic pefloxacin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Implementation Example 1 Preparation of Pefloxacin Molecularly Imprinted Electrochemical Sensor

[0014] 1. Electrode polishing

[0015] Take two gold electrodes with a diameter of 2 mm, soak them in Piranha solution for 15 min, and then use 0.3 μm and 0.05 μm Al 2 o 3 After the polishing powder is polished, it is ultrasonicated in absolute ethanol and ultrapure water for 5 minutes, and is scanned at -0.3-1.2V, 50mv / s until a stable cyclic voltammogram is obtained.

[0016] 2. Self-installation of electrodes

[0017] The polished electrode was placed in a 50 mmol / L ethanol solution of mercaptoaniline and incubated at room temperature for 24 h, and then washed with absolute ethanol and ultrapure water to remove mercaptoaniline not adsorbed by the gold electrode.

[0018] 3. Pefloxacin adsorption

[0019] The mercaptoaniline-modified gold electrode was placed in a pefloxacin solution containing 10 mmol / L, incubated at room temperature for 2 h, then washed with absolut...

Embodiment 2

[0023] Implementation Example 2 Application of Pefloxacin Molecularly Imprinted Electrochemical Sensor

[0024] 1. Drop the sample to be tested on the surface of the working electrode of the molecularly imprinted sensor, and incubate at room temperature for 15 minutes;

[0025] 2. Place the molecularly imprinted sensor in the electrolytic cell, and the detection bottom solution of the electrolytic cell is 2.5 mmol potassium ferricyanide buffer solution containing 0.1 mol / L KCl;

[0026] 3. Set appropriate scanning parameters and perform cyclic voltammetry (CV), alternating current impedance (EIS) and differential pulse (DPV) measurements.

[0027] The experimental results showed that the impedance value of the standard product pefloxacin increased with the increase of the concentration. Define the impedance value of the molecularly imprinted working electrode after washing off the template molecule pefloxacin R 0 , the electrode impedance value after the adsorption reaction ...

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Abstract

The invention relates to a molecular imprinting sensor for detecting antibiotic pefloxacin and a preparation method thereof. A molecular imprinting electrochemical sensor which can specifically identify the pefloxacin is prepared based on an electroplating process. According to the molecular imprinting electrochemical sensor, a gold electrode serves as a working electrode and is modified by p-aminothiophenol, so that binding sites of the pefloxacin are increased, mixed solution of chloroauric acid, p-aminothiophenol and tetrabutylammonium perchlorate serves as electroplating solution, a molecular imprinting identification film is prepared through electroplating, and the template pefloxacin in the molecular imprinting identification film is removed by employing an electrolytic dissociation method of hydrochloric acid. The prepared molecular imprinting electrochemical sensor is stable in structure, simple in preparation method, high in sensitivity and high in specificity and can be repeatedly used. The trace detection of pefloxacin in milk is realized, the detection sensitivity is improved, and the method is low in cost and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of rapid detection of antibiotics, in particular to a molecular imprinted sensor for detecting the antibiotic pefloxacin based on molecular imprinted specific recognition and a preparation method thereof. Background technique [0002] Pefloxacin is one of the fluoroquinolone antibiotics, which are derivatives of quinolones. In the past ten years, it has been widely used as an antibacterial drug to prevent and treat infectious diseases in humans and animals. Excessive use will lead to pefloxacin residues in animal tissues, resulting in drug resistance and some adverse reactions. The residue of pefloxacin in food has also attracted people's attention. Since this type of drug can be secreted through milk, the residue of this type of antibiotic in milk has become a concern of people. At the same time, pefloxacin can also cause some adverse reactions in the human body, such as dizziness, nausea, and some allerg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/48
Inventor 孙秀兰田秀梅张银志纪剑
Owner JIANGNAN UNIV
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