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Fluorescence sensor and preparation method and application thereof

A fluorescent sensor and reaction technology, applied in the field of fluorescent sensing, can solve the problems of expensive test equipment and low detection sensitivity, and achieve the effects of short detection time, good selectivity and great application prospects

Active Publication Date: 2020-01-17
HUBEI NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a fluorescent sensor for detecting dopamine in serum and its preparation method and application for the problems of expensive test equipment and low detection sensitivity in the current method for detecting DA in serum. The fluorescent sensor prepared by the present invention The content of dopamine in serum samples can be detected conveniently and concisely. This method has the advantages of cheapness, simplicity, sensitivity, good selectivity, wide linear range, and short detection time. It is useful in the detection of DA in the process of biological analysis or disease research. Larger application prospects

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  • Fluorescence sensor and preparation method and application thereof
  • Fluorescence sensor and preparation method and application thereof

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Embodiment 1

[0039] A fluorescent sensor in this embodiment, the fluorescent sensor is composed of a nanocomposite composed of aminated carbon quantum dots (N-CQDs) and p-aminothiophenol-modified silver nanoparticles (N-AgNPs).

[0040] A method for preparing a fluorescent sensor of the present embodiment comprises the following steps:

[0041] (1) Preparation of amidated carbon quantum dots (N-CQDs)

[0042] Weigh 0.72g of glucose and place it in a round bottom flask, add 25mL of ultrapure water to dissolve it, put it into a vacuum drying oven after ultrasonication for 10min, heat at 200°C for 3h, and cool the orange-yellow viscous liquid obtained after the reaction naturally to room temperature, after centrifugation and filtration, dilute to 25mL with ultra-pure water, and the obtained yellow solution is the CQDs mother liquor; take 5mL of the CQDs mother liquor into a 50mL round-bottomed flask, add 100μL ethylenediamine to it, and put it into In a vacuum drying oven, heat at 200°C for ...

Embodiment 2

[0059] The application of a fluorescent sensor of this embodiment in the detection of dopamine in serum comprises the following steps:

[0060] (1) Detection of dopamine content in standard solution

[0061] Take 200 μL of 5 copies of the fluorescent sensor prepared in Example 1, add 1.0 mL of dopamine standard solution samples with concentration gradients of 1.65 nM, 10 nM, 50 nM, 100 nM, 150 nM, 250 nM and 325 nM respectively, and use PBS with a concentration of 10 nM pH=8.0 The buffer solution was fixed to 5.0mL, and after reacting for 12min, under the excitation of 401nm, the fluorescence spectrum of the dopamine standard solution sample was detected with a fluorescence spectrophotometer. According to the concentration of the standard solution sample and the fluorescence spectrum F / F 0 The relationship between establishes a standard working straight line; where F 0 Represents the original fluorescence of the fluorescent sensor itself, and F represents the fluorescence spe...

Embodiment 3

[0068] In order to further study the practical application of N-CQDs / N-AgNPs nanocomposites, dopamine standard sample solution was added to 4 clinical serum samples respectively, and the DA content in the serum after the addition was detected by the prepared fluorescent sensor , the test results are shown in Table 2.

[0069] Table 2 The results of the standard recovery experiment

[0070]

[0071] The results in Table 2 show that the recovery rate is 98.8-109.8%, and the relative standard deviation (RSD) is less than 3.0%, which has good accuracy. Based on the above results, it is indicated that the N-CQDs / N-AgNPs nanocomposite fluorescent sensor can be well applied to the detection of DA in serum, which provides better application potential for medical clinical analysis.

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Abstract

The invention discloses a fluorescence sensor and a preparation method and application thereof. The fluorescence sensor is made from a nanocomposite consisting of aminated carbon quantum dots (N-CQDs)and p-aminothiophenol modified silver nanoparticles (N-AgNPs). During preparation, the aminated carbon quantum dots are prepared firstly, then the p-aminothiophenol modified silver nanoparticles areprepared, and finally the N-CQDs / N-AgNPs nanocomposite fluorescence sensor is prepared from the aminated carbon quantum dots and the p-aminothiophenol modified silver nanoparticles. The fluorescence sensor can be used for detecting the content of dopamine (DA) in serum; the prepared fluorescence sensor can detect the dopamine content in a serum sample conveniently and simply; and the method has the advantages of being cheap, simple, high in sensitivity and selectivity, wide in linear range, short in detection time and the like and has huge application prospects in DA detection in the biological analysis or disease research process.

Description

technical field [0001] The invention relates to the technical field of fluorescence sensing, in particular to a fluorescence sensor and its preparation method and application. Background technique [0002] Dopamine (DA) is an important hormone and neurotransmitter among catecholamines and phenethylamines, which plays many regulatory roles in the human brain and controls many physiological functions, such as motivation, emotion, endocrine regulation and movement ; Many diseases, such as Parkinson's disease, schizophrenia and anorexia are associated with abnormal levels of DA. In order to study the biological function of DA in the life process, establishing a method for testing DA has always been one of the hot spots in the field of analysis. However, the normal concentration of DA in biological systems ranges from 0.1 mM to 1.0 mM, which is a very low concentration range. Therefore, sensitive detection of DA remains challenging. [0003] So far, the methods for detecting D...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432G01N2021/6417
Inventor 吴一微王秋月占鑫
Owner HUBEI NORMAL UNIV
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