Method for preparing protein nitrogen source applicable to fermentation and food by rice
A protein nitrogen and rice technology, applied in food preparation, application, food science, etc., can solve the problems of insufficient nitrogen source, dark syrup appearance, and low boiling temperature
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Embodiment 1
[0031] (1) Mix 100 parts by weight of rice flour with water, stir for 0.5 hour, control the slurry concentration to be 30%, adjust the pH value of the slurry to 6.1~6.4, add calcium chloride, Ca 2+ Termamyl 120L high-temperature α-amylase was added in an amount of 0.02 parts by weight, liquefied by continuous spraying at 110°C and heat preservation treatment at 98-100°C for 140min.
[0032] (2) Saccharification: Cool the liquefied liquid to 61°C-63°C, pH value is 4.1-4.5, add 0.02 parts by weight of Dextrozyme DX 1.5X glucoamylase (containing glucoamylase and pullulanase) or 0.04 parts by weight of Dextrozyme GA glucose For amylase, after 10 hours of hydrolysis time, 0.001 parts by weight of NovoCorADL acid lipase was added to carry out synergistic hydrolysis, and the hydrolysis was continued for 60 hours.
[0033] (3) Heating: adjust the pH value of the saccharification solution obtained in step (2) to 5.1-5.4 to the isoelectric point of the protein, heat to 90°C-95°C, keep i...
Embodiment 2
[0040] (1) Mix 100 parts by weight of rice flour with water, stir for 0.5 hour, control the slurry concentration to be 35%, adjust the pH value of the slurry to 6.3~6.4, add calcium chloride, Ca 2+ Termamyl 120L high-temperature α-amylase was added in an amount of 0.1 parts by weight, continuously sprayed at 112°C for liquefaction and kept at 98-100°C for 100 minutes.
[0041] (2) Saccharification: Cool the liquefied liquid to 63°C-65°C, pH value is 4.1-4.5, add 0.002 parts by weight of Dextrozyme DX 1.5X glucoamylase (containing glucoamylase and pullulanase) and 0.1 parts by weight of DextrozymeGA glucose starch Enzyme, after 10 hours of hydrolysis time, add 0.001 weight part of Lipex lipase to carry out synergistic hydrolysis, and continue hydrolysis for 60 hours.
[0042] (3) Heating: adjust the pH value of the saccharification solution obtained in step (2) to 5.1-5.4 to the isoelectric point of the protein, heat to 90°C-95°C, keep it for 20-25 minutes, and let stand to pre...
Embodiment 3
[0049] (1) Mix the rice flour of 100 parts by weight with water, stir for 0.5 hour, control the slurry concentration to be 42%, adjust the pH value of the slurry to 6.1~6.3, add calcium chloride, Ca 2+ The content of Termamyl 2Xα-amylase is about 45mg / kg, adding Termamyl 2Xα-amylase in an amount of 0.8 parts by weight, continuous jet liquefaction at 110°C-112°C and heat preservation treatment at 98°C-100°C for 40min.
[0050] (2) Saccharification: Cool the liquefied liquid to 58°C-62°C, pH value is 5.2-5.4, add 1 weight part of malt, 0.08 weight part of Promozyme D2 debranching enzyme, and 0.09 weight part of Palatase 20000L lipase for synergistic hydrolysis , hydrolyzed for 25 hours.
[0051] (3) Heating: heat the saccharification solution to 90°C-95°C, keep it for 20-25 minutes, and let it stand for filtration;
[0052] (4) Use a filter to filter, wash with hot water, reclaim residual sugar, and obtain sugar grains;
[0053] (5) Further hydrolysis and separation of fat: ad...
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