Improved chocolate agar culture medium and preparation method thereof

An agar medium and chocolate technology, applied to bacteria and other directions, can solve the problems of inability to grow, missed or misdiagnosed, difficult clinical microbiological testing, etc., and achieves the effects of simple preparation method, accurate testing results, and strong operability.

Inactive Publication Date: 2009-10-07
王惠云 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the culture medium produced by many microbial reagent manufacturers on the market has low nutrient content and no uniform regulations on the proportion of each component. During the experiment, Haemophilus cannot grow well in this medium, or even cannot grow. It has brought a lot of difficulties to the clinical microbiological examination, and also brought a lot of trouble to the clinician's diagnosis, which can easily lead to missed or misdiagnosed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take 47 grams of CM375 (BRAIN HEART INFUSION AGAR) and add 1000 grams of distilled water to dissolve, autoclave for 30 minutes, cool to 50°C, add 73 grams of defibrated sheep blood into the ultra-clean workbench, take it out and put it into a shaking shaker, 70°C, shake at a frequency of 110 times / min for 30 minutes, then place in a water tank at 50°C, bathe in water for 30 minutes, and then pack aseptically.

Embodiment 2

[0022] Take 47 grams of CM375 (BRAIN HEART INFUSION AGAR) and add 1000 grams of distilled water to dissolve, autoclave for 35 minutes, cool to 45°C, add 83.7 grams of defibrated sheep blood in the ultra-clean workbench, take it out and put it into a shaking shaker, 65°C, shake at a frequency of 110 times / min for 40 minutes, then place it in a water tank at 50°C, bathe in water for 40 minutes, add 45 mg of vancomycin in sterile subpackages.

Embodiment 3

[0024] Take 30 grams of CM375 (BRAIN HEART INFUSION AGAR) and add 1000 grams of distilled water to dissolve, autoclave for 40 minutes, cool to 60°C, add 50.9 grams of defibrated sheep blood in the ultra-clean workbench, take it out and put it in a shaking table, 60°C, shake at a frequency of 100 times / min for 50 minutes, then place it in a water tank at 50°C, bathe in water for 1 hour, add 30 mg of vancomycin, and aseptically dispense it.

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PUM

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Abstract

The invention discloses an improved chocolate agar culture medium and a preparation method thereof. The culture medium is prepared by the materials with the following weight percentage: 4 to 5 percent of CM375, 6 to 8 percent of defibering sheep blood, 0.0030 to 0.0050 percent of vancocin and the balance of water. Pharmacological test and clinical trial show that the improved chocolate agar culture medium has abundant nutrient components; the mixture ratio of each component is scientific and reasonable; and strains like hemophilus can grow well in the culture medium and have high survival probability. The improved chocolate agar culture medium brings convenience for clinic microbiological examination and the diagnosis of clinic doctors, and ensures the accuracy of the examination effect. Meanwhile, the materials of the improved chocolate agar culture medium are easily obtained, the preparation method technique of the improved chocolate agar culture medium is simple and the maneuverability is strong; moreover, the invention is suitable for industrial production.

Description

technical field [0001] The invention relates to a medium, in particular to an improved chocolate agar medium and a preparation method thereof. Background technique [0002] At present, the culture medium produced by many microbial reagent manufacturers on the market has low nutrient content and no uniform regulations on the proportion of each component. During the experiment, Haemophilus cannot grow well in this medium, or even cannot grow. It has brought a lot of difficulties to clinical microbiological examination, and also brought a lot of trouble to clinicians' diagnosis, which is easy to cause missed diagnosis or misdiagnosis. Contents of the invention [0003] Purpose of the invention: the purpose of the present invention is to provide a kind of improved chocolate agar medium in order to solve the deficiencies in the prior art, and another object of the present invention is to provide a kind of method for preparing improved chocolate agar medium. [0004] Technical ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 王惠云张爱华钟天鹰迟富丽徐飞黄松明
Owner 王惠云
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