Cleaner production method of gellan gum

A clean production, gellan gum technology, applied in the field of bioengineering, can solve problems such as efficient treatment of unfavorable organic wastewater, heavy treatment load and economic cost, achieve obvious environmental and social benefits, reduce construction investment and operating costs, reduce The effect of water consumption

Inactive Publication Date: 2009-10-21
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Remaining distilled water is rich in high concentration of total phosphorus (>10mg / L), total nitrogen up to 200-500mg / L, COD up to 7000-20000mg / L, BOD up to 5000-15000mg / L, which brings great harm to the wastewater treatment in the subsequent process. Heavy treatment load and economic cost, and rich in high concentration of phosphorus, are not conducive to the efficient treatment of organic wastewater

Method used

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  • Cleaner production method of gellan gum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: the preparation of gellan gum fermentation broth

[0015] The gellan gum-producing strain is P. elodea or Sphingomonas paucimobilis ATCC 31461.

[0016] Seed medium (kg / m 3 ): Sucrose 20; Peptone 5.0; Beef Extract 3.0; Yeast Extract 1.0; pH 7.0.

[0017] Initial fermentation medium (kg / m 3 ): corn starch 30; soybean meal 3.0; K 2 HPO 4 1.0; KH 2 PO 4 3.0; MgSO 4 ·7H 2 O 1.0; pH 7.0.

[0018] Fermentation conditions: the fermentation process is carried out in a fermenter of 5 cubic meters, the inoculum size is 5% (v / v), the stirring speed is 300r / min, the ventilation rate is 1.0vvm, the tank pressure is 0.05MPa, and the fermentation temperature is 30°C. During the fermentation process, NaOH solution was used to adjust the pH value of the fermentation broth. After 18 hours, the pH value was kept constant at about 5.6. After 72 hours of fermentation and cultivation, the gellan gum production and quality control parameters were measured.

Embodiment 2

[0019] Embodiment 2: the product preparation of gellan gum

[0020] Measure 1000 liters of gellan gum fermentation liquid, slowly add 2000 liters of ethanol solvent, stir and mix well and let it stand for 30 minutes, then continuously centrifuge under the condition of 6000r / min to obtain about 250kg of solid matter, the supernatant is ethanol solution, and transport it to waste Ethanol storage tank. Gained solids are transported to a washing tank, add 400 liters of ethanol solvent, stir and mix well and let stand for 30 minutes, then centrifuge continuously at 6000r / min to obtain 150kg of solids and transport them to a vacuum dryer. The supernatant is ethanol waste The liquid is transported to the waste ethanol storage tank for centralized recycling. The solid matter obtained above was vacuum-dried for 6 hours at a temperature lower than 42°C, the solution obtained by vacuum condensation was transported to a waste ethanol storage tank, and the dried solid matter was crushed a...

Embodiment 3

[0021] Embodiment 3: the product preparation of low acyl degree gellan gum

[0022] Adjust the pH of the fermented mash obtained in Example 1 to 10.0 with 0.3 mol / L NaOH solution, raise the temperature to 80°C and keep it warm for 25 minutes, and then treat it with the method described in Example 2 after cooling to obtain low-acidity gelatin Glue products.

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Abstract

The invention relates to a cleaner production method of gellan gum, belonging to the technical field of biotechnology. In the method, microorganism for producing gellan gum suffers from ventilating and fermenting; isopropanol or ethanol is added into the obtained ferment broth to precipitate and centrifugalize; precipitate is vacuumed, dried and ground; gellan gum product is obtained. Supernatant fluid obtained by centrifugation is rectified to recover isopropanol or ethanol which can be circularly utilized for the next precipitation. Residual distilled water after rectification is analyzed and brought into loop fermentation medium, cornstarch, soyabean expeller and other raw materials are added, gellan gum ferment broth is obtained after ventilation and fermentation to form a closed-circuit cleaner production method. In the invention, under the condition of not increasing investment on equipment and site, cyclic utilization of residual distilled water is realized by adjusting production technique structure of gellan gum, which can not only save raw materials cost, reduce usage amount of water in technique, but also greatly lowers process load of waste water, particularly brings down process load and discharge amount of phosphorus and nitrogen.

Description

technical field [0001] The invention discloses a clean production method of gellan gum, which belongs to the technical field of bioengineering. Background technique [0002] Gellan gum (CAS number: 71010-52-1) is a microbial heteropolysaccharide produced by aerobic fermentation of Pseudomonas elodea or Sphingomonas paucimobilis, and its main chain structure is It consists of a linear tetrasaccharide repeating unit, each tetrasaccharide repeating unit includes 2 β-D-glucose, 1 β-D-glucuronic acid and 1 α-L-rhamnose. The 2 and 6 hydroxyl groups of the glucose molecule in the natural gellan gum molecular structure unit are often replaced by acetyl groups and glycerol acyl groups to form esters. These acyl groups can be hydrolyzed under alkaline conditions to obtain low acyl gellan gum. The molecular weight of gellan gum is about 500,000 Da, which belongs to sphingolipid polysaccharide. Gellan gum has been approved as a food additive by the US FDA, Japan, Germany, Australia, S...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12P19/04C12R1/38C12R1/01
Inventor 詹晓北郑志永
Owner JIANGNAN UNIV
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