21-trisomy syndrome prenatal screening kit

A technology for trisomy syndrome and prenatal screening, which is applied in the direction of DNA/RNA fragments, microbial measurement/testing, recombinant DNA technology, etc., can solve the problems of high cost, high technical requirements, and unsuitability for clinical promotion and use, and achieve Simplified analysis steps, high sensitivity, and easy-to-obtain results

Inactive Publication Date: 2009-10-21
PEKING UNIV THIRD HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Of course, interphase amniotic fluid cells can also be used for fluorescence in situ hybridization without culture, so as to provide rapid prenatal diagnosis, but FISH requires high technical requirements and relatively high costs, so it is not suitable for clinical promotion.

Method used

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  • 21-trisomy syndrome prenatal screening kit
  • 21-trisomy syndrome prenatal screening kit
  • 21-trisomy syndrome prenatal screening kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1、21- 3

[0041] The preparation of embodiment 1, 21-trisomy syndrome prenatal screening kit

[0042] The kit consists of four pairs of primers and auxiliary components:

[0043] Primer pair A: 5'-TAT GTG AGT CAA TTC CCC CAA GTG A-3' (upstream primer);

[0044] 5'-GTT GTA TTA GTC AAT GTT CTC CAG (downstream primer).

[0045] Primer pair B: 5'-GTA GAT ACA TAC ATA TGA TGA ATG C-3' (upstream primer);

[0046] 5'-TAT TAA TGT GTG TCC TTC CAG GC-3' (downstream primer).

[0047] Primer pair C: 5'-CGG AGG TTG CAG TGA GTT G-3' (upstream primer)

[0048] 5'-GGG AAG GCT ATG GAG GAG A-3' (downstream primer).

[0049] Primer pair D: 5'-CCC TCT CAA TTG TTT GTC TAC C-3' (upstream primer)

[0050] 5'-GCA AGA GAT TTC AGT GCC AT-3' (downstream primer).

[0051] The 5' ends of the upstream primers of the four pairs of primers were all labeled with a FAM reporter fluorophore.

[0052] Auxiliary components: 10×PCR buffer, MgCl 2 , Taq enzyme, dNTP, ddH 2 O. ...

Embodiment 2、21- 3

[0053] Example 2, Application of Trisomy 21 Prenatal Screening Kit

[0054] 1. Prenatal screening for trisomy 21 using the kit prepared in Example 1

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Abstract

The invention discloses a 21-trisomy syndrome prenatal screening kit. The kit provided by the invention comprises a primer pair A, a primer pair B, a primer pair C and a primer pair D; the primer A consists of nucleotides represented by a sequence 1 in a sequence table and nucleotides represented by a sequence 2 in the sequence table; the primer pair B consists of nucleotides represented by a sequence 3 in the sequence table and nucleotides represented by a sequence 4 in the sequence table; the primer pair C consists of nucleotides represented by a sequence 5 in the sequence table and nucleotides represented by a sequence 6 in the sequence table; and the primer pair D consists of nucleotides represented by a sequence 7 in the sequence table and the nucleotides represented by a sequence 8 in the sequence table. The kit of the invention applied to 21-trisomy syndrome prenatal screening has the advantages of innovating specimen material, making prenatal screening earlier, reducing specimen acquisition amount, reducing diagnosis time, and judging extra chromosome resources. The kit has far-reaching significance for finding the causes of chromosomal disorder and in-time prevention and control of the chromosomal disorder.

Description

technical field [0001] The invention relates to a prenatal screening kit for trisomy 21 syndrome. Background technique [0002] According to statistics, 1.3% of the approximately 20 million babies born in my country each year have congenital birth defects, 80% of which are genetic diseases. Chromosomal diseases are the most common type of genetic diseases that cause birth defects in newborns. Chromosomal disorders usually include abnormalities in chromosome number and chromosome structure. Most chromosomal diseases are abnormal chromosome numbers. Abnormal chromosome numbers are common in trisomy and haplotype in chromosomal aneuploidy. Trisomies of each chromosome can appear, and more than 95% of trisomies are aborted before birth. The most common trisomy syndromes in live-born neonates include 21-trisomy syndrome (Down'Syndrome), 18-trisomy syndrome (Edwards Syndrome), 13-trisomy syndrome (Patau Syndrome), Klinefilter syndrome. [0003] Trisomy 21, also known as Down syn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 迟洪滨程利南乔杰
Owner PEKING UNIV THIRD HOSPITAL
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