21-trisomy syndrome prenatal screening kit
A technology for trisomy syndrome and prenatal screening, which is applied in the direction of DNA/RNA fragments, microbial measurement/testing, recombinant DNA technology, etc., can solve the problems of high cost, high technical requirements, and unsuitability for clinical promotion and use, and achieve Simplified analysis steps, high sensitivity, and easy-to-obtain results
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Embodiment 1、21- 3
[0041] The preparation of embodiment 1, 21-trisomy syndrome prenatal screening kit
[0042] The kit consists of four pairs of primers and auxiliary components:
[0043] Primer pair A: 5'-TAT GTG AGT CAA TTC CCC CAA GTG A-3' (upstream primer);
[0044] 5'-GTT GTA TTA GTC AAT GTT CTC CAG (downstream primer).
[0045] Primer pair B: 5'-GTA GAT ACA TAC ATA TGA TGA ATG C-3' (upstream primer);
[0046] 5'-TAT TAA TGT GTG TCC TTC CAG GC-3' (downstream primer).
[0047] Primer pair C: 5'-CGG AGG TTG CAG TGA GTT G-3' (upstream primer)
[0048] 5'-GGG AAG GCT ATG GAG GAG A-3' (downstream primer).
[0049] Primer pair D: 5'-CCC TCT CAA TTG TTT GTC TAC C-3' (upstream primer)
[0050] 5'-GCA AGA GAT TTC AGT GCC AT-3' (downstream primer).
[0051] The 5' ends of the upstream primers of the four pairs of primers were all labeled with a FAM reporter fluorophore.
[0052] Auxiliary components: 10×PCR buffer, MgCl 2 , Taq enzyme, dNTP, ddH 2 O. ...
Embodiment 2、21- 3
[0053] Example 2, Application of Trisomy 21 Prenatal Screening Kit
[0054] 1. Prenatal screening for trisomy 21 using the kit prepared in Example 1
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