Prewarning method for miichthys spotted maigre bacterial gill-rot disease
A yellow croaker and bacterial technology, which is applied in the field of early warning of aquatic animal diseases, can solve problems such as economic loss and disease occurrence of farmers, and achieve the effects of low cost, convenient detection and operation, and ordinary storage conditions.
Inactive Publication Date: 2009-10-28
ZHEJIANG OCEAN UNIV
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Problems solved by technology
The croaker carp is mainly cultured in seawater cages. Although the disease-resistant ability of yellow croaker is strong, with the continuous expansion of the breeding scale, the problem of disease occurrence is becoming more and more serious, which has caused great economic losses to the farmers.
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[0020] The early-warning method of bacterial gill rot of yellow croaker has the following steps:
[0021] ①Preparation of fluorescent antibody: Take the serum of diseased fish, add adjuvant, inject it into purebred New Zealand rabbits weighing 3kg, prepare rabbit anti-fish serum antibody, and label the above antibody with fluorescent dye to obtain a labeling solution for use.
[0022] The above-mentioned adjuvant contains 1 part of lanolin and 5 parts of paraffin oil in parts by volume.
[0023] Adjuvant preparation method: put lanolin and paraffin oil in a container according to the ratio, mix them evenly with ultrasonic waves, sterilize them under high pressure, and store them at 4°C for later use. Before immunization, take an equal volume of adjuvant and mix the immunogen solution, and mix with a shaker to form an emulsion. Before immunization, take the required amount of adjuvant and grind it in a mortar, and then add an equal volume of antigen solution (with BCG 4 mg / m...
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The invention discloses a prewarning method for miichthys spotted maigre bacterial gill-rot disease. The method comprises the following steps of: 1) preparing fluorescent antibodies; 2) purifying the fluorescent antibodies; 3) detecting and the like. As a great amount of immunofluorescence probe molecules can be obtained by preparing and purifying the fluorescent antibodies, the cost is low, the detecting time is short, and precious time for disease prewarning and prophylaxis and treatment is obtained; furthermore, the method also can detect infected individuals with bacteria or without invasion, and the two states are more dangerous in practice in latency. Therefore, the method can help raisers to reduce cultivation risk greatly with lower cost by detecting an individual which is to be detected and probably has bacterial gill-rot disease causal factors periodically so as to take evadable measures in time. Besides, the preservation condition of the prepared immunofluorescence probe molecules is more common, and the detection operation is more convenient, thus being applicable to popularization.
Description
technical field [0001] The invention relates to an early warning method for aquatic animal diseases, in particular to Early warning method of bacterial gill rot in croaker fish. Background technique [0002] Nibea miichthioides Chu Lo&Wu belongs to the genus Nibea miichthioides Chu Lo&Wu, and is commonly known by fishermen along the coasts of Fujian and Guangdong. Perch, weighing up to 25 to 30kg, has delicious meat, and its swim bladder has medicinal value, and is sold well in the international market. The croaker carp is mainly cultured in seawater cages. Although croaker has strong disease resistance, with the continuous expansion of the breeding scale, the problem of disease occurrence is becoming more and more serious, which has caused great economic losses to the farmers. Effective control of bacterial fish diseases is a necessary measure to improve production and management benefits. [0003] Bacterial fish diseases have a wide range of hazards and serious t...
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Inventor 吴常文于新秀徐佳晶
Owner ZHEJIANG OCEAN UNIV