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Protein of adjusting plant tolerant to low-phosphorus stress and its coding gene and application thereof

A gene-encoded, low-phosphorus-resistant technology, applied in the field of bioengineering, can solve problems such as unknown effects

Inactive Publication Date: 2009-12-16
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its role in plant tolerance to low phosphorus stress is unknown

Method used

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  • Protein of adjusting plant tolerant to low-phosphorus stress and its coding gene and application thereof
  • Protein of adjusting plant tolerant to low-phosphorus stress and its coding gene and application thereof
  • Protein of adjusting plant tolerant to low-phosphorus stress and its coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, the acquisition of MAX4 and its coding gene

[0029] About 50-100 mg of wild-type Colombian ecotype Arabidopsis seedlings were used as materials, the total RNA was extracted with Trizol, and the RNA concentration was detected with an ultraviolet spectrophotometer. Press RevertAid TM First Strand cDNA SynthesisKit (Fermentas Company) kit manual, using the extracted total RNA as a template to synthesize the first strand of cDNA. Using the extracted first-strand cDNA as a template, perform the following PCR reaction: 20 μl reaction system, containing 2 μl of 10×PCR buffer, 0.4 μl of dNTPs (10 mM) mixture, 2 μl each of primer 1 and primer 2, and Tag enzyme (5 U / μl) 0.2μl, and add double distilled water to the rest to 20μl. Among them, primer 1: 5'-TGCCCTCTTTCCAATACACTG-3', primer 2: 5'-AACTGTATCCACCCAATCCG-3'. Pre-denatured at 94°C for 3 minutes, then 30 sec at 94°C, 30 sec at 54°C, and 60 sec at 72°C for a total of 35 cycles, and finally extended at 72°C...

Embodiment 2

[0030] Example 2, Cultivation of Arabidopsis thaliana with Enhanced Low Phosphorus Tolerance

[0031] 1. The acquisition of homozygous mutants max4-7 and max4-8 with the MAX4 gene knocked out

[0032] Two T-DNA insertion mutant seeds were obtained from the American Arabidopsis Germplasm Center (SALK_072708, max4-7; SALK_147538, max4-8; http: / / www.arabidopsis.org / servlets / TairObject?type=germplasm&id =4626493). In order to identify homozygous mutants whose MAX4 gene has been knocked out, the seeds of SALK_072708 and SALK_147538 were planted on soil culture, the RNA of a single plant was extracted, and after reverse transcription into cDNA, the cDNA was used as a template to perform PCR with the following primers Amplification: max4-7, primer 1 LBb1: 5'-GCGTGGACCGCTTGCTGCAACT-3, primer 2: MAX4-7LP 5'-AGTACGACGACGTATTGTCCG-3', primer 3: MAX4-7RP 5'-GGATTGATGCTGCACATATCC-3'; max4-8, primer 1LBb1: 5'-GCGTGGACCGCTTGCTGCAACT-3, primer 4: MAX4-8LP 5'-LPAGACAAACCCCATTCATCATG-3', prim...

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PUM

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Abstract

The invention discloses a protein of adjusting plant tolerant to low-phosphorus stress and its coding gene and application thereof. Plant low-phosphorus stress tolerant protein is SEQ ID No:2 in a sequence list, and the coding gene SEQ ID No:1 DNA sequence in the sequence list. The invention uses coding gene of low-phosphorus stress tolerant protein to enhance the low-phosphorus stress tolerant capacity of plants, such as suppression of the expression of the coding gene of the low-phosphorus tolerant related protein of plants, so as to provide gene and technical support for low-phosphorus tolerant breeding for crops.

Description

1. Technical field [0001] The invention relates to a plant low-phosphorus tolerance-related protein in the field of bioengineering, its coding gene and application, and a method for enhancing plant low-phosphorus tolerance by using the gene. 2. Background technology [0002] Although the phosphorus content in the soil is abundant, a large amount of phosphorus exists in a form that plants cannot directly use, so plants are often in a phosphorus-deficient environment. In order to increase crop yield, in traditional agriculture, a large amount of phosphorus fertilizer is often used to improve yield and quality, but the utilization rate of phosphorus is very low, generally only 10% to 25%, and nearly 75% to 90% is accumulated in the soil. Therefore, in addition to traditional agricultural breeding, it is of great theoretical and economic significance to use genetic engineering technology to improve the ability of crops to tolerate low phosphorus. The key to this work lies in the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82C12N15/83
Inventor 曹树青简红勇陈正义汪洋边小会江力袁怀波孙佳佳任永兵
Owner HEFEI UNIV OF TECH