CHO cell line expressing bovine beta interferon and application thereof

A technology of beta interferon and cell line, which is applied in the field of CHO cell line expressing chicken beta interferon and chicken beta interferon, can solve the problems of low product activity, complicated production process and product composition, and achieve simple process and high product composition. easily controlled effects

Inactive Publication Date: 2009-12-16
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The quantification of interferon requires a stable standard product. The vertebrate natural chicken leukocyte interferon in the current quality standards for veterinary biological products promulgated by the Ministry of Agriculture is obtained by inducing chicken leukocytes with chicken Newcastle disease virus. The production process and product components are complicated, and the final product Low activity, about 10000AU·mL -1
Compared with other expression systems, vertebrate interferon expressed by mammalian cells can be correctly folded, glycosylated and post-translationally modified, so it is more stable, has higher specific activity, and is more suitable as a standard product. Cell Vertebrate Chicken IFN-β Standard

Method used

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  • CHO cell line expressing bovine beta interferon and application thereof
  • CHO cell line expressing bovine beta interferon and application thereof
  • CHO cell line expressing bovine beta interferon and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0036] Example. Construction of CHO expression system for efficient and stable expression of recombinant chicken interferon beta and detection of biological activity

[0037] 1 Materials and methods

[0038] 1.1 Plasmids, cell lines and virus strains

[0039] Chicken red blood cells were collected from SPF chickens (commercially purchased from Harbin Veterinary Research Institute) and prepared according to conventional methods; Newcastle disease F48e9 strain was commercially purchased from Harbin Veterinary Research Institute, and cultured on primary chicken embryo fibroblasts (Chicken embryo fibroblasts, CEFs) Titrate half the tissue culture infectious dose (TissueCulture Infectious Dose 50 , TCID 50 ); H5N1 subtype avian influenza virus SW101 strain (SW101) and SW103 strain (SW103) were commercially purchased from Harbin Veterinary Research Institute.

[0040] The stable eukaryotic expression vector pCI-neo (pCN) was purchased from Promega, and neo was expressed under the...

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Abstract

The invention discloses a CHO cell line expressing vertebrate beta interferon, which contains genes coding beta interferon of the animal. The invention particularly discloses a CHO cell line expressing chicken beta interferon. The invention also relates to a method utilizing the CHO cell line to produce standard vertebrate beta interferon and chicken beta interferon obtained by the method, and the standard vertebrate beta interferon and chicken beta interferon have higher specific activity and stability, can be used as standard products for chicken beta interferon biological activity detection, can be used for preventing and treating chicken infectious diseases and has significant application prospect and market prospect.

Description

technical field [0001] The present invention relates to a CHO cell line expressing vertebrate interferon beta, which contains a gene encoding said animal interferon beta. More specifically, the present invention discloses a CHO cell line expressing chicken beta interferon. The present invention also relates to a method for producing a chicken interferon beta standard product using the above-mentioned CHO cell line and the chicken interferon beta obtained by the method, which has extremely high specific activity and stability and can be used as a biological agent for chicken beta interferon. It is used as a standard for activity detection and for the prevention and treatment of chicken infectious diseases. Background technique [0002] Type I interferon is an important part of the natural immune system of vertebrates and is an important research field of modern medicine. Nowadays, the mechanism of evading natural immunity such as interferon when the virus infects the host h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12N15/22C07K14/565C12P21/02G01N33/50A61K38/21A61P31/00A61P31/16
Inventor 步志高陈伟业
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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