Eukaryotic expression carrier for expressing double genes

Abstract

The invention discloses a eukaryotic expression carrier for expressing double genes. The sequence of the carrier contains a phytic acid enzyme gene expressing box and a human viscidity virus resistance gene 1 expressing box. On the cellular level, the expressing carrier verifies that the expressing efficiency of the expressing carrier containing double genes is almost equivalent to the expressingefficiency of two single-gene groups. In the invention, the AMP plasmid containing double genes is transferred into pig PK15 cells and prepares transgenic mice, and a condition that the efficiency ofthe expressing double genes, namely the phytic acid enzyme gene (appA) and the human viscidity virus resistance gene 1 (Mx1) of the AMP is almost equivalent to the expressing efficiency of the two single-gene groups is detected by quantitative PCR.

Description

A eukaryotic expression vector expressing double genes technical field The invention relates to a eukaryotic expression vector expressing double genes. Background technique In the process of studying gene function and transgenic animal preparation through overexpression technology, we often use eukaryotic expression vectors to achieve it. The traditional method is to connect the target gene fragment to the downstream of the eukaryotic promoter and integrate it into the cell genome by means of molecular biology. It can guide the expression of exogenous genes. This method is widely used in the study of gene expression and function, but this research method also has shortcomings: in many cases, the current research may need to study the interaction relationship between multiple genes. To study nuclear expression vectors, multiple vectors need to be constructed separately, and research at the cell level must be transfected multiple times and screened separately to obtain cell...

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Problems solved by technology

This method is widely used in the study of gene expression and function, but this research method also has shortcomings: in many cases, the current research may need to study the interaction relationship between multiple genes. To study nuclear expression vectors, multiple vectors need to be constructed separately, and research at the cell level must be transfected multiple times and screened separately to obtain cells that integrate multiple genes at the same time

Similarly, when preparing transgenic animals with different genes in transgenic animals, it is first necessary to prepare single-gene transgenic animals separately, and screen homozygotes separately, and then cross the homozygotes to obtain offspring that can express multiple genes at the same time. Long cycle, cumbersome operation and high cost

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