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Enrichment and transformation of mulberry leaf mushrooms, cultivation method and use thereof

A cultivation method and technology for the mushroom, which are applied in the field of mushroom and its cultivation, can solve the problems of unstable quality, slow effect of mulberry leaves and the like, and achieve the effect of lowering blood sugar.

Active Publication Date: 2011-12-28
JIANGSU ANHUI BIO TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although mulberry leaves have these effects, mulberry leaves also have the common shortcomings of traditional Chinese medicines such as slow effect and unstable quality.

Method used

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  • Enrichment and transformation of mulberry leaf mushrooms, cultivation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The tissue separation of embodiment 1 Coprinus comatus bacterial classification

[0024] Take the fresh unopened fruiting body of Hebei wild Coprinus comatus, cut off the base of the stipe and the outer skin of the stipe, disinfect the surface of the fruiting body with 75% ethanol, move it into the ultra-clean workbench, and irradiate it with ultraviolet light for 20 minutes. Burn the scalpel on the flame of an alcohol lamp and put it in sterile water to cool down to ensure that the temperature of the scalpel will not damage the fruiting body tissue. Divide the fruiting body into two parts, cut the epidermal tissue in the stipe (in the stipe), the junction of the stipe and the cap (junction), the cap and the gills, and cut the small pieces of soybean size. solid surface texture. Use sterile tweezers to pick up the slant culture medium (that is, PDA medium) of the test tube, pick up 10 of each tissue, culture in the dark at 25-28°C, and regularly observe the growth and ...

Embodiment 2

[0025] Example 2 Preparation of Coprinus comatus Fruiting Body Enriched and Transformed with Mulberry Leaves

[0026] 1, insert the Coprinus comatus bacterial classification that embodiment 1 obtains in the newly prepared potato culture medium (Zhuge Jian, Wang Zhengxiang, Industrial Microbiology Experimental Technology Handbook, 1994: P 367), 20 ℃ of culture temperature, treat that mycelium grows After the slant is full, the slant strains are inserted into a seed bottle with a water content of 50% bran medium, cultivated at 20°C for 120 days;

[0027] 2. Mix 300Kg of wheat straw, 15Kg of waste yeast, 3Kg of ammonium sulfate, 15Kg of mulberry leaf powder, 6Kg of lime, and 390Kg of water to form a haystack with a height of 0.8 meters, a width of 1 meter, and a length of 4 meters for fermentation. The maximum temperature is 60°C. 21 days, including turning once on the 7th, 13th, and 18th days. After 21 days; the material is paved into a fermentation bed with a length of 10 mete...

Embodiment 3

[0029] Example 3 Preparation of Coprinus comatus Fruiting Body Enriched and Transformed with Mulberry Leaves

[0030] 1, insert the Coprinus comatus strain that embodiment 1 obtains in the newly prepared potato culture medium (Zhuge Jian, Wang Zhengxiang, Industrial Microbiology Experimental Technology Handbook, 1994: P 367), 25 ℃ of culture temperature, treat that mycelium grows After the slant is full, the slant strains are inserted into a seed bottle with a water content of 65% bran medium, cultivated at 27°C for 90 days;

[0031]2. Mix 300Kg of wheat straw, 120Kg of waste yeast, 15Kg of ammonium sulfate, 30Kg of mulberry leaf powder, 15Kg of lime, and 600Kg of water to form a haystack with a height of 1.5 meters, a width of 1.5 meters, and a length of 6 meters. 21 days, including turning once on the 7th, 13th, and 18th days. After 21 days; the material is paved into a fermentation bed with a length of 20 meters, a width of 1.5 meters and a thickness of 0.20 meters, and th...

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Abstract

The invention discloses a Coprinus comatus for enriching and transforming mulberry leaves and its production method and use. By inserting the strains on the inclined plane of Coprinus comatus test tube into newly prepared bran, wheat grain or cottonseed hull culture medium, it will be used for cultivation The medium is stacked and fermented, and after stacking and fermented, the strains are inserted by sowing or hole-sowing, and the fruiting bodies are obtained by heat-preserving and moisturizing culture. The obtained fruiting bodies of Coprinus comatus can be made into medicine or health food which can regulate blood sugar. Coprinus comatus obtained in the present invention has multiple medicinal functions, can be used for blood sugar control and treatment of diabetic patients and patients with abnormal glucose tolerance, and can also be used to improve the body immune function of normal people to prepare and prevent and / or treat diabetic patients and patients with abnormal glucose tolerance Drugs or functional foods for patients and diabetic complications, especially its ability to lower blood sugar in diabetic patients, making it an ideal drug or health food for lowering blood sugar.

Description

Technical field: [0001] The invention relates to a Coprinus comatus and its cultivation method and application. Background technique: [0002] Diabetes is a common and frequently-occurring disease among middle-aged and elderly people. The complications caused by diabetes seriously endanger people's life and health. The existing western medicine hypoglycemic drugs have good effects and clear targets, but long-term use may easily cause side effects. Traditional Chinese medicine has few side effects, wide sources and low cost, and is a main source of medicine for treating diabetes. [0003] Mulberry leaves are the dried leaves of the Moraceae plant Morus alba. As a traditional Chinese medicine, mulberry leaves have been widely used among the people. Since ancient times, Chinese medicine has used mulberry leaves to treat thirst syndrome (equivalent to diabetes in modern medicine). "Wood Cao Gang Day" records: mulberry leaves "juice decocted instead of tea, can stop thirst". ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04C05G1/00C05F17/00A61K36/06A61P3/10A23L19/00
CPCY02W30/40
Inventor 陈惠张志才郑惠华陆汉萍
Owner JIANGSU ANHUI BIO TECH
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