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Transformated mulberry leaf enriching coprinus comatus, cultivating method and application thereof

A technology of chicken leg mushrooms and mulberry leaves, which is applied in the directions of botanical equipment and methods, application, and preparation of organic fertilizers, can solve the problems of unstable quality, slow effect of mulberry leaves, etc., and achieves the effect of lowering blood sugar

Active Publication Date: 2009-12-30
JIANGSU ANHUI BIO TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although mulberry leaves have these effects, mulberry leaves also have the common shortcomings of traditional Chinese medicines such as slow effect and unstable quality.

Method used

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  • Transformated mulberry leaf enriching coprinus comatus, cultivating method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Tissue separation of Coprinus comatus strains

[0024] Take fresh unopened fruit bodies of Hebei wild Coprinus comatus, trim off the base of the stalk and the outer skin of the stalk, disinfect the surface of the fruit bodies with 75% ethanol, move them to an ultra-clean workbench, and irradiate them with ultraviolet light for 20 minutes. Burn the scalpel on the flame of an alcohol lamp and put it in sterile water to cool down to ensure that the temperature of the scalpel will not damage the fruit body tissue. Divide the fruit body into two, cut the inner tissue of the epidermis in the stalk (in the stalk), the junction of the stalk and the cap (junction), the cap and gills, small pieces the size of soybeans, and the seeds cannot be cut through when cutting Solid surface organization. Use sterile tweezers to connect the test tube slant culture medium (that is, PDA culture medium), each tissue is connected to 10 pieces, 25-28 ℃ dark culture, regularly observe the g...

Embodiment 2

[0025] Example 2 Preparation of Coprinus comatus fruiting bodies enriched and transformed into mulberry leaves

[0026] 1. Connect the Coprinus comatus strain obtained in Example 1 to the newly prepared potato culture medium (Zhuge Jian, Wang Zhengxiang, Industrial Microbiology Laboratory Technology Manual, 1994: P 367), and the culture temperature is 20°C. When the mycelium grows After the slope is full, insert the slope bacteria into a seed bottle containing a bran medium with a water content of 50%, and cultivate at a temperature of 20°C for 120 days;

[0027] 2. Wheat straw 300Kg, waste yeast 15Kg, ammonium sulfate 3Kg, mulberry leaf powder 15Kg, lime 6Kg, and water 390Kg are evenly mixed and piled into a haystack with a height of 0.8 meters, a width of 1 meter and a length of 4 meters for fermentation, the highest temperature is 60 ℃, fermentation 21 days, of which the piles were turned once on the 7, 13, and 18 days. 21 days later; the material is spread into a fermentation ...

Embodiment 3

[0029] Example 3 Preparation of Coprinus comatus fruiting bodies enriched with transformed mulberry leaves

[0030] 1. Connect the Coprinus comatus strain obtained in Example 1 to the newly prepared potato culture medium (Zhuge Jian, Wang Zhengxiang, Industrial Microbiology Experiment Technical Manual, 1994: P 367), and the culture temperature is 25°C. When the mycelium grows After the slope is full, insert the slope bacteria into a seed bottle containing a bran medium with a water content of 65%, and cultivate at a temperature of 27°C for 90 days;

[0031]2. Wheat straw 300Kg, waste yeast 120Kg, ammonium sulfate 15Kg, mulberry leaf powder 30Kg, lime 15Kg, water 600Kg mix evenly to form a haystack with a height of 1.5 meters, a width of 1.5 meters, and a length of 6 meters for fermentation, the highest temperature is 70 ℃, fermentation 21 days, of which the piles were turned once on the 7, 13, and 18 days. 21 days later; the material was spread into a fermentation bed with a lengt...

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Abstract

The invention discloses transformated mulberry leaf enriching coprinus comatus, a cultivating method and application thereof. The method comprises the following steps of: inoculating slant strains in a coprinus comatus tube in a newly prepared culture medium of bran, wheat grain or cotton seed hull for culturing, piling and fermenting the culture medium used for cultivation, inoculating the strains by sowing or hole sowing after the piling fermentation, and obtaining a fruitbody of the coprinus comatus by the moisture-retention and heat-insulation culture. The obtained fruitbody of the coprinus comatus can be prepared into a medicament or health care food with the function of regulating the blood sugar. The coprinus comatus prepared by the method has the functions of various medicaments, and can be used for blood sugar-controlling and treating of diabetic patients and patients with abnormal glucose tolerance, can be also used for improving the immunologic function of human bodies of normal crowd, and can be prepared into the medicament or functional food for preventing and / or treating the diabetic patients, the patients with the abnormal glucose tolerance and patients with diabetes-related complications, and particularly, the coprinus comatus has the effect of reducing the blood sugar of the diabetic patients, so that the coprinus comatus becomes the ideal medicament or the health care food for reducing the blood sugar.

Description

Technical field: [0001] The invention relates to a Coprinus comatus and its cultivation method and application. Background technique: [0002] Diabetes is a common and frequently-occurring disease in middle-aged and elderly people. The complications caused by diabetes seriously endanger people's life and health. The existing western medicine hypoglycemic drugs have good effects and clear targets, but long-term use is easy to cause side effects. Traditional Chinese medicine has small side effects, a wide range of sources, and low cost. It is a major source of medicine for the treatment of diabetes. [0003] The mulberry leaf is the dried product of the leaves of Morus alba. As a traditional Chinese medicine, mulberry leaf has been widely used in the folk. Since ancient times, traditional Chinese medicine has used mulberry leaves to treat Diabetes (equivalent to diabetes in modern medicine). "Wood Grass Gang Ri" records: Mulberry leaves "juice decoction instead of tea can quench th...

Claims

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Application Information

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IPC IPC(8): A23L1/212A23L1/29C05G1/00C05F17/00A01G1/04A61K36/06A61P3/10A23L19/00
CPCY02W30/40
Inventor 陈惠张志才郑惠华陆汉萍
Owner JIANGSU ANHUI BIO TECH
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