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Method for preparing transparent xanthan gum by utilizing microorganism fermentation

A technology of microbial fermentation and xanthan gum, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as poor light transmittance, and achieve the effect of low cost and simple production process

Inactive Publication Date: 2009-12-30
HEBEI XINHE BIOCHEM
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  • Abstract
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Problems solved by technology

[0002] The production of xanthan gum is to inoculate the production bacteria in the sterile medium containing carbon source, nitrogen source, inorganic salt and water for fermentation. The fermentation process is aerobic fermentation under the specified temperature and pH conditions. Due to the use of organic nitrogen (soybean powder) as the main nitrogen source of the fermentation medium in the prior art, there is a problem of poor light transmittance of the product

Method used

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Examples

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Embodiment Construction

[0040] The present invention will be further described below in conjunction with the examples, but not as a limitation of the present invention.

[0041] The overall process steps of this embodiment are as follows:

[0042] The method for preparing transparent xanthan gum by microbial fermentation comprises the following process steps:

[0043] A, the original bacterial classification is made the slant bacterial classification;

[0044] B, the inclined-plane bacterial classification in step A is made seed liquid through expanding culture;

[0045] C, the seed liquid fermentation medium prepared in the step B is inserted into the fermentation medium to ferment, and the fermentation liquid containing xanthan gum is made;

[0046] The nitrogen source of the fermentation medium in step C is inorganic nitrogen, the inoculum size is 9:100, the initial pH of the fermentation medium is 7.0-7.2, the fermentation condition is a temperature of 28°C-32°C, the fermentation period is 55 h...

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Abstract

The invention relates to a method for synthesizing required compound by adopting fermentation, in particular to a method for preparing transparent xanthan gum by utilizing microorganism fermentation. The invention comprises the following steps: selecting and using xanthomonas campestris as production strain; preparing the slant strain; preparing the slant strain into seed liquid by intermediate culture; connecting the seed liquid fermentation medium into the fermentation medium for fermentation; preparing the fermentation liquid containing xanthan gum. The invention solves the problem of poor transmittance of the products existing in the prior art; the invention has the advantages that the technology is simple and reasonable, the production cost is low, and xanthan gum with higher transmittance can be extracted from the fermentation liquid prepared by the invention.

Description

technical field [0001] The invention relates to a method for synthesizing required compounds by fermentation, in particular to a method for preparing transparent xanthan gum by fermentation with microorganisms. Background technique [0002] The production of xanthan gum is to inoculate the production bacteria in the sterile medium containing carbon source, nitrogen source, inorganic salt and water for fermentation. The fermentation process is aerobic fermentation under the specified temperature and pH conditions. The prior art has the problem of poor light transmittance of the product due to the use of organic nitrogen (soybean powder) as the main nitrogen source of the fermentation medium. Contents of the invention [0003] The object of the present invention is to provide a method for preparing transparent xanthan gum by microbial fermentation, using inorganic nitrogen as the main nitrogen source, so that the light transmittance of the product is improved. [0004] Over...

Claims

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Application Information

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IPC IPC(8): C12P19/06C12R1/64
Inventor 张禹张国佩张少华刘学珍
Owner HEBEI XINHE BIOCHEM
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