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Preservation and controlled delivery/release of spermatozoa

A sperm and semen technology, applied in the field of biopolymer particles, can solve the problems of poor efficacy

Active Publication Date: 2009-12-30
斯博姆维透公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Munkittrick et al. (1992) and Vishwanath et al. (1997) reported that when encapsulated spermatozoa were inseminated simultaneously with unmanipulated spermatozoa, it was less effective than the latter

Method used

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  • Preservation and controlled delivery/release of spermatozoa
  • Preservation and controlled delivery/release of spermatozoa
  • Preservation and controlled delivery/release of spermatozoa

Examples

Experimental program
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Effect test

preparation example Construction

[0070] The preparation of alginate beads is known to those skilled in the art, for example O. and G. (1990) Alginate as immobilization matrix forcells. Trends in Biotechnology 8, 71-78 and as disclosed in US 6,497,902.

[0071] A variety of gel solutions can be applied to form biopolymer particles, such as alginate beads. A variety of solutions suitable for forming particles of various biopolymers are well known to those skilled in the art. According to the invention, divalent ions (also referred to herein as gelling ions) such as calcium and barium may be used to form alginate particles, for example to effect gelation of said alginate. Alginate forms gels in the presence of various divalent and multivalent ions. The use of calcium ions resulted in rapid gelation and the formation of relatively strong particles. On the other hand, the use of barium ions forms even stronger particles that are more stable under physiological conditions. It will be appreciated that the typ...

Embodiment 1

[0107] Example 1 Immobilization of spermatozoa in alginate

[0108] Materials and methods

[0109] Material

[0110] The following chemicals were used: from Riedel de (Seelze, Germany) CaCl 2 ·H 2 O, K 2 HPO 4 , NaH 2 PO 4 , NaCl and sodium citrate; dextrose monohydrate from Norsk Medisinaldepot (Oslo, Norway); sodium alginate (PROTANAL LF 10 / 60) supplied by FMC Biopolymer A / S (Drammen, Norway);

[0111] sperm source

[0112] bovine sperm in Acquisition at Geno Experimental Field (Genofacility) in Trondheim, Norway. Boar spermatozoa were collected at Norsvin Experimental Farm, Hamar, Norway. Bovine spermatozoa were diluted 1+2 in milk diluent shortly after ejaculation. Boar spermatozoa were diluted 1+4 in TRI X-CELL (IMV Technologies, L'Aigle Cedex, France). This dilution or choice of dilution buffer is not critical to the immobilization procedure or the long-term survival of the sperm after immobilization, but merely to facilitate storage of the sperm until fur...

Embodiment 2

[0150] Embodiment 2 The energy consumption of immobilizing spermatozoa

[0151] Without being bound by a particular theory, it is believed that immobilization of spermatozoa results in a reduction in the energy expended by the spermatozoa, which is beneficial for storage life and their fertilization capacity.

[0152] Therefore, in order to study this theory, bovine spermatozoa were collected at about 150×IO 6 The concentration of spermatozoa / ml was fixed in alginate pellets and added to 2 times the volume of the beads in milk dilution buffer. A reference sample containing free-suspended spermatozoa was prepared from the same ejaculate at Each total volume of milk dilution buffer contains the same total sperm concentration. at 20°C, prior to storage, under N 2 The samples were prepared under anaerobic conditions of airflow. The milk dilution buffer was sampled during storage and the lactate produced by the sperm in the sample was measured by HPLC. The results are shown in ...

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Abstract

The present invention relates to biopolymer particles for preservation of spermatozoa, wherein the spermatozoa are embedded within the biopolymer particle. The present invention also regards a method for preservation, storage and controlled delivery / release of spermatozoa, and the use of the biopolymer particles according to the present invention in breeding.

Description

technical field [0001] The present invention relates to biopolymer particles for the preservation of spermatozoa. The invention also relates to methods for the preservation, storage and controlled delivery / release of spermatozoa, and the use of the biopolymer particles of the invention in breeding. Background technique [0002] Artificial insemination (AI) is a technique in which sperm are placed in the uterus or cervix of an animal by artificial means rather than by natural mating. It is widely used as a mating method and is used in animal breeding to reproduce desired traits, especially for farm animals such as cattle, pigs, sheep, poultry and horses, and for pets such as purebred dogs, aquatic animals or endangered species. [0003] Typically, sperm are collected, diluted, and then preserved, for example, by cryopreservation. The application of cryopreservation presupposes that spermatozoa from a particular animal species are tolerant to this treatment without undue de...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor E·科米斯拉德P·O·霍夫莫G·科林肯博格
Owner 斯博姆维透公司
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