Microbial online observation device

Abstract

The invention discloses a microorganism online observation device which can simulate high-pressure extreme environment and mainly comprises a sealed observer, wherein the observer is internally provided with a culture chamber and an observation chamber, one end of the culture chamber is connected with a transition joint in a sealed way, the other end of the culture chamber is communicated with the observation chamber, a position on the wall of the observer, which corresponds to the observation chamber, is provided with an observation hole, the observation hole is communicated with the observation chamber and is internally provided with a first transparent device, and the first transparent device and the observation chamber are arranged together in a sealed way. The invention has convenient connection, simple operation and wider application, not only can be used for singly carrying out high-pressure anaerobe culture and online observing high-pressure microorganism in real time, but also can be connected with a high-pressure flow system via a pressure pipe and a pressure switch to realize long-time continuous online culture and online micro observation.

Description

technical field [0001] The invention relates to a microscopic observation device for microorganisms in an extremely high-pressure environment, in particular to an online microscopic observation device for marine organisms under the conditions of high hydrostatic pressure and other abiotic ecological factors. Background technique [0002] The research and development of marine microbial resources is one of the themes of biotechnology in this century, and the research on microorganisms under the extreme environment of high pressure and low temperature in the deep sea is a hot spot among them. In 1949, Zobell and Johnson first proposed the concept of "barophile" (hereinafter also called piezophile), and in 1957, they obtained evidence of bacterial barophilic growth through the mixed culture of microorganisms in deep-sea sediments. In 1979, Yayano first reported the pure culture of barophiles. Since the 1980s, high-pressure separation and culture techniques have been used to is...

AI Technical Summary

Problems solved by technology

At present, among related equipment, there is no device for online microscopic observation of microorganisms under high pressure conditions.

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