Method for producing feed protein by fermenting swill

A technology for feed protein and swill, applied in the field of comprehensive utilization of catering waste, can solve the problems of resource waste, environmental pollution, high treatment costs and so on

Inactive Publication Date: 2010-02-17
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Direct discarding will cause serious environmental pollution and waste of resources. What's more, a few unscrupulous businessmen do not hesitate to entrap consumers to recycle the oil for profit
Patent CN101129159A utilizes Aspergillus niger and yeast to ferment citrus waste residue to produce single-cell feed. The fungal protein content of the feed is ≥20%, and the cellulose degradation rate is ≥5%. Sludge treatment of high-concentration swill to obtain auxiliary water for reclaimed water quality standards; however, failure to fully utilize swill still requires high treatment costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] Non-limiting examples are described as follows:

[0013] 1. Swill pretreatment

[0014] Take the swill and filter it with six layers of gauze, sterilize the filtered swill at 121°C for 5 minutes, and store it at 4°C for later use.

[0015] 2. Preparation of swill medium

[0016] Take 2 liters of swill and add 1 liter of water (swill content 0.66L / L), add 42g (14g / L) of sucrose, 37g (12.6g / L) of ammonium nitrate, and adjust with 0.05mol / L hydrochloric acid and 0.1mol / L NaOH solution. pH 6.2, sterilized at 121°C for 20 minutes, ready for use.

[0017] 3. Seed cultivation

[0018] Inoculate Aspergillus niger on PDA medium, cultivate it in an incubator at 28°C to produce spores, then wash it with sterile water and dilute it into a conical flask with glass beads, and place it on a 200r / min shaker to break up the spores And counted under the microscope, the suspension contains about 1.4×10 8 Spores / ml.

[0019] 4. Fermentation culture and post-treatment

[0020] Put 3L...

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PUM

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Abstract

The invention relates to a method for producing feed protein by fermenting swill, which comprises the steps of swill pretreatment, aspergillus niger seed culture, fermentation culture and post treatment. The invention is characterized in that the aspergillus niger seed liquid is inoculated in a swill culture medium for fermentation culture for 55-65 hours under the conditions that the temperatureis 28-32 DEG C and the ventilation volume is 2-4L / min. The swill culture medium comprises 0.6-0.8 g / L swill, 12-16g / L carbon source and 10-14g / L nitrogen source, and the pH value is 6.0-6.5. The mycelia are separated, washed and dried after the fermentation process is finished; the yield is larger than or equal to 20g / L, and the content of the protein in the mycelia is 56.8-66%; and moreover, essential amino acids are relatively complete, thus products can be used as feed or food additives. The swill is clear and transparent and has no abnormal taste after being fermented, COD is reduced by 80-95%, BOD5 is reduced by 85-92.6%, and the pollution caused by direct discharge of the swill is greatly reduced.

Description

1. Technical field [0001] The invention relates to a method for comprehensive utilization of catering waste, in particular to a method for producing feed protein by fermenting swill. 2. Background technology [0002] Swill refers to the leachate or dilution of leftover soup leftovers discarded in canteens, restaurants, etc. Mainly contains: starch, protein, oil, cellulose and other organic matter. On the one hand, swill has a high content of organic substances and less toxic and harmful substances; but on the other hand, swill is perishable, bad and smelly, which seriously pollutes the environment. [0003] At present, restaurants and canteens treat swill and catering solid waste by discarding or collecting them and directly using them as feed for pigs, chickens, ducks, etc. Feeding poultry and livestock with swill may cause them to be infected with zoonotic diseases such as foot-and-mouth disease and hepatitis B Infectious diseases seriously endanger human health. Direct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/10A23K10/12A23K10/20A23K20/147A23K20/163A23K20/22
Inventor 叶明刘冬
Owner HEFEI UNIV OF TECH
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