Flooded gum clone WLEG-17 tissue culture medium

A clone and culture medium technology, applied in plant regeneration, horticulture, horticultural methods, etc., can solve the problems of slow elongation growth, vitrification, large leaves, etc., achieve robust growth, control callus and vitrification effects.

Inactive Publication Date: 2010-03-03
CHONGQING UNIV OF ARTS & SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, when MS medium is used for tissue culture of Eucalyptus giantis clone WLEG-17, the following problems often occur: weak growth of tissue cultured seedlings, large leaves, small stems, slow elongation and growth, and easy callus after multiple subcultures and vitrification

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  • Flooded gum clone WLEG-17 tissue culture medium
  • Flooded gum clone WLEG-17 tissue culture medium
  • Flooded gum clone WLEG-17 tissue culture medium

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Embodiment Construction

[0033] In order to make the object, technical solution and advantages of the present invention clearer, preferred embodiments of the present invention are described in detail below.

[0034] 1. Experimental materials

[0035] The tissue-cultured subculture seedlings obtained from the in vitro bud organ induction and multiplication of Eucalyptus macrophylla clone WLEG-17 in the tissue culture room of the Chongqing University Forestry and Flower Engineering Research Center were used as experimental materials.

[0036] 2. Experimental method

[0037] According to the results of the pre-experiment and long-term practical experience, the basic formula of the tissue culture medium of Eucalyptus gigantic clone WLEG-17 was determined (see Table 1), and then the random block design was adopted to divide the concentrations of the four macroelement components in the basic formula by The arithmetic difference of 1 / 8 is continuously increased twice and decreased twice, while other compone...

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Abstract

The invention discloses a flooded gum clone WLEG-17 tissue culture medium which consists of the following components by concentration: 1900-2375mg / L of KNO3, 273-411mg / L of NH4NO3, 270-338mg / L of KH2PO4, 480-540mg / L of MgSO4.7H2O, 300mg / L of Ca(NO3)2.4H2O, 6mg / L of VB2, 0.5mg / L of 6-BA, 0.2mg / L NAA and 4700mg / L of agar; the species and concentration of microelement, ferric salt and organic mattersas well as the concentration of cane sugar are the same as that of an MS culture medium; on the basis of the MS culture medium, the culture medium adjusts the species and concentration of macroelements, adds organic supplementary element VB2, optimizes hormone regulation and agar concentration, and the like, thus providing balanced and reasonable nutritional requirements for the flooded gum cloneWLEG-17 tissue culture seedling, ensuring fast, high efficient and healthy growth thereof, and effectively controlling the generation of healing and vitrifying phenomenon.

Description

technical field [0001] The invention relates to a eucalyptus tissue culture medium, in particular to the tissue culture medium of Eucalyptus grandis clone WLEG-17. Background technique [0002] Eucalyptus is one of the three major fast-growing tree species in the world. Among them, Eucalyptus grandis clone WLEG-17 (scientific name: Eucaiyptus grandis Hill ex Maiden) is the most important fast-growing and high-yield afforestation species in the tropical and subtropical regions of southern my country. , straight stem shape, beautiful tree shape, good material, resistance to diseases and insect pests, high temperature and drought resistance, and relatively poor resistance. Since conventional cutting propagation cannot meet the demand for large-scale afforestation for Eucalyptus giantis clone WLEG-17 seedlings, at present, tissue culture techniques are mostly used for factory breeding to obtain a large number of plants with the same genetic background and strong growth vigor in a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘奕清陈泽雄廖林正吕玉奎黄登艳
Owner CHONGQING UNIV OF ARTS & SCI
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