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Mixed paraffin, preservation method of PCR reagent and reagent box

A technology for reaction reagents and preservation methods, which is applied in biochemical equipment and methods, microbial determination/inspection, fermentation, etc., can solve problems such as long-term preservation, and achieve the effect of simple manufacturing method and low cost

Active Publication Date: 2010-03-10
深圳生科原生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The first purpose of the embodiment of the present invention is to provide a mixed paraffin for isolating the enzyme in the PCR reaction from other components, aiming to solve the problem of long-term preservation of the PCR reaction solution under low temperature conditions

Method used

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  • Mixed paraffin, preservation method of PCR reagent and reagent box
  • Mixed paraffin, preservation method of PCR reagent and reagent box
  • Mixed paraffin, preservation method of PCR reagent and reagent box

Examples

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Embodiment 1

[0028] Solid paraffin (Shanghai Specimen Model Factory) and liquid paraffin (Tianjin Fuchen Chemical Reagent Co., Ltd.) were heated and melted at a ratio of 1:4 (W / V), mixed evenly, and mixed paraffin was prepared.

[0029] Carry out the experiment according to the method for the determination of paraffin wax in "Chinese Pharmacopoeia (Second Edition)", use a capillary tube with openings at both ends, about 10mm in height, place it in a refrigerator at 4°C for 24 hours, and bind the capillary tube tightly with a rubber ring after the mixed paraffin wax solidifies On a thermometer, the contents of the capillary are attached to the middle of the thermometer bulb. Immerse the capillary together with the thermometer in the temperature transfer liquid, the upper end of the mixed paraffin should be about 10mm below the temperature transfer liquid surface; heat carefully, and when the temperature rises to about 5°C lower than the lower limit of the melting point, adjust the heating ra...

Embodiment 2～10

[0031] Basically the same as Example 1, the difference is that the ratios of solid paraffin and liquid paraffin in Examples 2 to 10 are successively 1:5, 1:6, 1:7, 1:8, 1:8.5, 1:9, 1:10 , 1:11, 1:12 (W / V). The melting points of mixed paraffins are shown in Table 1.

[0032] Table 1

[0033] Example

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Abstract

The invention provides a mixed paraffin, a method for storing a PCR reagent by adopting the mixed paraffin, and a PCR reagent box containing the mixed paraffin; the mixed paraffin is formed by the uniform mixing of liquid paraffin and solid paraffin after melting, wherein the solid paraffin is selected from the solid paraffin with the melting point higher than 50 DEG C, and the ratio between the solid paraffin and the liquid paraffin is 1:4 to 1:12(w / v). The melting point of the mixed paraffin is between 27 DEG C and 38 DEG C, the mixed paraffin is used for isolating enzyme and other components in PCR liquid, and the melting point is moderate, thus not influencing the effect of fluorescent quantitative PCR. Furthermore, the mixed paraffin in the embodiment of the invention has simple production method and low cost.

Description

technical field [0001] The invention relates to a mixed paraffin, a method for storing PCR reaction reagents by using the mixed paraffin, and a PCR reaction kit containing the mixed paraffin. Background technique [0002] Real-time fluorescent quantitative PCR (Real Time Polymerase Chain Reaction, Real Time PCR real-time PCR) technology is a method for quantitative or qualitative analysis of the starting template by real-time detection of the fluorescence signal of each cycle product in the PCR amplification reaction. At present, it is widely used in basic scientific research, clinical diagnosis, disease research and drug development and other fields. The basic principle is: a fluorescent chemical substance is introduced into the PCR reaction system, and as the PCR reaction progresses, the PCR reaction product accumulates continuously, and the fluorescence signal intensity also increases proportionally. After each cycle, a fluorescence signal intensity is collected, so that...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/34C12Q1/68
Inventor 张鹏汪再兴吴炳义彭论严琼英
Owner 深圳生科原生物有限公司
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