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Method for assistantly sieving soybean with lower plant height and special primer thereof

A high-character, soybean technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. High trait difficulties and other problems, to reduce waste and improve breeding efficiency

Inactive Publication Date: 2010-03-17
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Soybean plant height is greatly affected by the ecological environment and fluctuates greatly between years, so it is very difficult to select soybean plant height traits by conventional methods

Method used

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  • Method for assistantly sieving soybean with lower plant height and special primer thereof
  • Method for assistantly sieving soybean with lower plant height and special primer thereof

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Experimental program
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Effect test

Embodiment 1

[0020] Embodiment 1, the acquisition of quantitative trait genetic loci (htL_1) closely linked with soybean plant height

[0021] 1. Construction of Charleston recombinant inbred line and identification of plant height at maturity

[0022] 1. Construction of Charleston recombinant inbred lines

[0023] The hybrid F 1 ; Obtain progenies by self-pollination of hybrid F1 generation, and obtain 143 F 12 generation of recombinant inbred lines.

[0024] 2. Identification of plant height of Charleston recombinant inbred line at mature stage

[0025] Plant 143 F in Hulan, Suihua and Harbin respectively 12 generation of recombinant inbred lines. By 3 meters row length, 6cm plant distance sowing, 51 plants in each row, each strain is repeated three times (ie three rows); random block group. When the soybeans were fully mature, 10 individual plants were randomly selected from each family in each place, and the measurement was repeated three times.

[0026] 2. Genetic map construct...

Embodiment 2

[0032] Embodiment 2, the verification of the quantitative genetic locus htL_1 of controlling soybean plant height

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Abstract

The invention discloses a method for assistantly sieving soybean with lower plant height trait and a special primer thereof. The method comprises the following steps: the genomic DNA of soybean to betested is adopted as a template, the primer is used for carrying out PCR amplification on a party A and a party B respectively and an amplified product of the soybean to be tested is obtained; the genomic DNA of Charleston is adopted as a template, the primer is used for carrying out PCR amplification on the party A and the party B respectively and an amplified product of the Charleston is obtained; carrying out electrophoresis on the amplified products of the soybean to be tested and the Charleston, if presenting same band type, the soybean to be tested is candidate soybean with lower plant height trait; a primer pair A consists of nucleotides shown in a sequence 1 of a sequence table and a sequence 2 of the sequence table; and a primer pair B consists of nucleotides shown in a sequence 3of the sequence table and a sequence 4 of the sequence table. The invention also provides a quantitative trait locus of the soybean with height plant, which is positioned at an L linkage group and ispositioned between Sat_009 and Sat113 of the SSR primer locus. The method can judge whether the quantitative trait locus (QTL)-htL_1 is contained by DNA measurement of soybean variety resource or filial generation, thus predicting the plant height level of the soybean and accelerating and improving breeding speed of the plant height of the soybean.

Description

technical field [0001] The invention relates to a method for assisting in screening soybeans with lower plant height traits and special primers thereof. Background technique [0002] Soybean plant height has an important impact on the formation of yield, and the study of its developmental genetics is helpful to understand the mechanism of yield formation. Soybean plant height is a quantitative trait controlled by polygenes. People have done a lot of research on its genetic law with traditional statistical analysis methods. Wang Lianzheng and others summarized the previous research results and believed that the heritability of plant height was between 45% and 75%. Chen Henghe pointed out that the offspring of soybean high-short-stalk hybrids conformed to the segregation ratio controlled by one or two pairs of genes, but most of the hybrid offspring showed a continuous distribution controlled by multiple genes. Yang Qingkai et al. believe that the additive gene effect of pla...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 李文滨韩英鹏滕卫丽孙德生杜玉平张忠臣
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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