A kind of detection method of persimmon leaf extract
A technology of persimmon leaf extract and detection method, which is applied in the field of quality detection of persimmon leaf extract, can solve problems such as the inability to confirm ethyl acetate, and the quality of persimmon leaf extract cannot be accurately evaluated, and achieve the effect of accurate evaluation
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Embodiment 1
[0037] Since ethyl acetate has been used many times in the extraction process of the persimmon leaf extract, ethyl acetate will remain in the persimmon leaf extract and affect the quality of the persimmon leaf extract, and the present invention detects the persimmon leaf extract by gas chromatography Ethyl acetate residue in the extract to be tested.
[0038] The specific steps are: accurately weigh 0.2g of the persimmon leaf extract to be tested, grind it, and then place it in the first empty bottle, then accurately weigh 2ml of N,N-dimethylformamide, and place it in the first empty bottle , shake well, and seal to obtain the test solution.
[0039] Precisely measure 2ml of ethyl acetate reference substance, dilute it with N,N-dimethylformamide to make a solution containing 0.5ml per 1ml, precisely measure 2ml of the above dilution, place it in a second empty bottle, and seal it as Reference substance solution.
[0040] Measure according to gas chromatography (appendix V E)...
Embodiment 2
[0042] Flavonoids are the main active ingredients in persimmon leaf extract, and the flavonoids in persimmon leaf extract are mainly quercetin and kaempferol aglycones and their glycosides. The total amount of quercetin and kaempferin after hydrolysis of persimmon leaf extract can basically reflect the total flavonoid content in persimmon leaf extract, and can also reflect the total flavonoid content in persimmon leaf extract, so in this paper The invention also includes a method for determining the content of kaempferol and quercetin in the persimmon leaf extract by using high performance liquid chromatography. Specific steps are as follows:
[0043] Octadecylsilane-bonded silica gel was used as a filler, and methanol and 0.2 wt% phosphoric acid were mixed in a weight ratio of 50:50 as a mobile phase.
[0044] Take kaempferol and quercetin respectively, accurately weigh them, mix them with methanol, and configure a mixed solution containing 50 μg of each kaempferol and querc...
Embodiment 3
[0049] In addition to the determination of ethyl acetate residue by gas chromatography of persimmon leaf extract and the determination of kaempferin and quercetin content in persimmon leaf extract to be tested by high performance liquid chromatography, the present invention also includes identification of persimmon by thin layer chromatography. Steps of leaf extract.
[0050] The specific steps are: take 0.2g of the persimmon leaf extract to be tested, grind it finely, mix it with 25ml of methanol, and ultrasonically treat it for 30 minutes, filter the above methanol mixture, evaporate the filtrate to dryness, add 15ml of water to the remaining residue, stir fully to dissolve , filter, add 0.5ml of hydrochloric acid to the filtrate, shake and extract with 30ml of anhydrous ether, separate the ether layer, evaporate to dryness, add 1ml of methanol to dissolve the residue, and use it as the test solution.
[0051] Take another 8 g of persimmon leaf extract as reference medicinal...
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Abstract
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