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Method for extracting ursolic acid from loquat leaf

A technology of loquat leaves and ursolic acid is applied in the field of extraction and purification of ursolic acid, which can solve the problems of many solvents and complicated operations, and achieve the effects of high product yield, easy recovery of reagents, and simple impurity removal process.

Inactive Publication Date: 2010-05-12
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are also some methods that use a lot of solvents, and the operation is complicated

Method used

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  • Method for extracting ursolic acid from loquat leaf
  • Method for extracting ursolic acid from loquat leaf

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Get 1kg dry loquat leaves (ursolic acid content 0.81%), pulverize to 30 orders, use 10L methanol reflux extraction 1 hour, add 50g gac in the extract, decolorize 0.5 hour under stirring state, the solution after adsorption is concentrated under reduced pressure to The alcohol content is 71%, crystallized at a constant temperature of 50° C., and the crystal is refluxed with 95% methanol to be saturated, dissolved, and recrystallized twice at a constant temperature to finally obtain 522 g of ursolic acid product with a content of 98.3%.

Embodiment 2

[0028] Get 1kg dry loquat leaves (ursolic acid content 0.92%), grind to 40 orders, use 11L methanol reflux extraction 1.25 hours, add 100g gac in the extract, decolorize 0.75 hours under stirring state, the solution after adsorption is concentrated under reduced pressure to The alcohol content is 73%, crystallized at a constant temperature of 52°C, and the crystal is refluxed with 97% methanol for saturation dissolution and recrystallized twice at a constant temperature, finally obtaining 586g of ursolic acid product with a content of 98.7%.

Embodiment 3

[0030] Get 1kg dry loquat leaves (ursolic acid content 0.98%), pulverize to 50 orders, use 12L methanol reflux extraction 1.5 hours, add 75g gac in the extract, decolorize 1 hour under stirring state, the solution after adsorption is concentrated under reduced pressure to The alcohol content is 70%, crystallized at a constant temperature of 55°C, and the crystal is refluxed with 99% methanol to saturate, dissolve, and recrystallized twice at a constant temperature to finally obtain 614g of ursolic acid product with a content of 99.4%.

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Abstract

The invention relates to a method for separately extracting ursolic acid which is low in cost, high in output and short in period, and is easy to realize industrialization. The method comprises the technical steps of extracting, decoloring, condensing and recrystallizing. The method for producing ursolic acid has the advantages of less solvent kinds, simple process, high yield and extremely high purity.

Description

Technical field: [0001] The invention relates to an extraction and purification process of ursolic acid, in particular to a method for extracting ursolic acid from fresh Eucommia leaves. Background technique: [0002] Ursolic acid is a pentacyclic triterpenoid compound present in natural plants, also known as ursolic acid and ursolic acid. Molecular formula is C 30 h 48 o 3 , the structure is as follows: [0003] [0004] Its high content is glossy prismatic (absolute ethanol) or fine hair-like needle crystal (dilute ethanol), and low content is brownish yellow or yellowish green powder with special smell. Melting point is 283~288℃. α] D25+67.5° (C=1, in 1mol / L potassium hydroxide alcoholic solution). At 15°C, 1 ml of methanol (99%) can dissolve 10 mg, and 1 ml of boiling water can dissolve 45 mg. Ursolic acid is distributed in about 7 families, 46 genera and 62 species of plants in the free form or in the form of glycosides combined with sugars. Mainly leaves of p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00
Inventor 刘东锋张翼杨成东
Owner NANJING ZELANG MEDICAL TECH
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