Bensulfuron-methyl artificial immunogen BE-BSA, preparation thereof and application thereof

Abstract

The invention relates to bensulfuron-methyl artificial immunogen BE-BSA, a preparation thereof and application thereof. The preparation for a structural formula comprises the following steps: (1) dissolving bensulfuron-methyl in solution of NaOH for reaction, extracting and separating the solution by chloroform, acidifying an aqueous layer, filtering the solution under reduced pressure, and drying the filtrated solution in vacuum to obtain a purified product; and (2) dissolving bensulfuron-methyl hapten, N-hydroxy succinimide (NHS) and N,N'-dicyclohexyl carbimide (DCC) in an organic solvent, stirring and reacting the mixture, and centrifugating the solution and taking supernatant to obtain active ester; and dissolving bovine serum albumin (BSA) in borate buffer solution (pH 8), adding the active ester into solution of protein under strong stirring, dialyzing the solution after reacting, packing the dialyzed product, and preserving the product at the temperature of 20 DEG C below zero. The preparation is simple and convenient, has low cost, and is easy for industrialized production; and the bensulfuron-methyl artificial immunogen BE-BSA is prepared into a bensulfuron-methyl specific antibody through immune animals for detecting trace bensulfuron-methyl in water body and soil on site.

Description

technical field The invention belongs to the field of polyclonal antibodies of bensulfuron-methyl, in particular to a bensulfuron-methyl artificial immunogen BE-BSA and its preparation and application. Background technique Bensulfuron-methyl is a new type of sulfonylurea herbicide developed by DuPont in the mid-1980s. It is currently one of the main herbicides widely used in rice field weed control. Bensulfuron-methyl is a systemic herbicide, which has a good control effect on annual and perennial broad-leaved weeds and Cyperaceae weeds. Such as sedge, sedge, sedge, moshangcai, sagittaria, etc., and weeds of the sedge family such as goosegrass, special-shaped sedge, water sedge, broken rice sedge, and firefly. It has poor control effect on gramineous weeds, and has a certain inhibitory effect on barnyardgrass at high doses. The use of bensulfuron-methyl is flexible, and it can be used as fertilizer, soil, sand, spraying, watering, etc., and the dosage is 20-30g / hm 2 , ca...

AI Technical Summary

Problems solved by technology

The technical problem to be solved in the present invention is to provide a kind of bensulfuron-methyl artificial immunogen BE-BSA and its preparation and application, to overcome the complex, time-consuming and large amount of pretreatment of bensulfuron-methyl samples in the existing detection technology. Organic solvent extraction, and the use of sophisticated and expensive instruments in the detection process are not suitable for popularization and use, and are not conducive to rapid on-site detection when environmental pollution accidents occur in the field.

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