Mixed bactericide for restoring places polluted by polynuclear aromatic hydrocarbons, preparation method and application method

A polycyclic aromatic hydrocarbon and mixed bacterial agent technology, which is applied in the field of bioremediation of organically polluted sites, can solve problems such as poor degradation effect, and achieve the effects of loose texture, improved degradation ability and high activity

Inactive Publication Date: 2010-06-16
INST OF GEOGRAPHICAL SCI & NATURAL RESOURCE RES CAS
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] It can be seen from the above that most of the bacterial agents currently used for PAH pollution remediation use one or two types of bacteria or fungi to degrade a certain type of low-ring or high-ring PAHs. In fact, industrial polluted sites often All 16 or more PAHs exist at the same time, so the degradation effect is poor

Method used

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  • Mixed bactericide for restoring places polluted by polynuclear aromatic hydrocarbons, preparation method and application method
  • Mixed bactericide for restoring places polluted by polynuclear aromatic hydrocarbons, preparation method and application method
  • Mixed bactericide for restoring places polluted by polynuclear aromatic hydrocarbons, preparation method and application method

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Experimental program
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Effect test

Embodiment 1

[0027] Embodiment 1 Preparation of mixed bacterial fermentation broth

[0028] The bacteria used are Bordetella, Ochrobactrum, Rhodococcus, Achcromobacter, Herbaspirillum, and Microbacterium. They are respectively cultured on inclined planes, activated in shake flasks, and fermented to obtain bacterial fermentation broth. The specific steps are as follows:

[0029] (1) Incline medium

[0030] Prepare beef extract-peptone solid medium by conventional methods, the specific steps are: beef extract 2.5g, peptone 5g, NaCl 5g, distilled water 1000ml, pH7.2-7.4, agar 1.5%-2%, stir evenly, sterilize at 121.3°C Bacteria 25min. After sterilization, make inclined planes and place them on ultra-clean workbenches. Aseptically inoculate the preserved strains on the inclined test tube, culture the strains at 25-30°C for 36-48 hours, and wait for the bacterial lawn to cover the inclined surface for later use.

[0031] (2) Preparation of seed solution

[0032] The liquid medium is still be...

Embodiment 2

[0035] Example 2 Degradation efficiency verification test of liquid bacterial agent

[0036] In the polycyclic aromatic hydrocarbon liquid culture medium of 4.4mg / L, add respectively the mixed bacterial liquid of three kinds of proportioning described in embodiment with 2% inoculum size, after 18d, the degradation rate of total polycyclic aromatic hydrocarbon is respectively 49.4%, 50.9% and 47.4%.

Embodiment 3

[0037] Embodiment 3 The preparation of solid composite bacterial agent, such as figure 1 shown.

[0038] (1) Preparation of composite carrier:

[0039] Peat soil and activated carbon were obtained from Beijing Hualihang Technology Development Co., Ltd., and rice husk and sawdust were obtained from Beijing Fangshan Institute of Agricultural Sciences.

[0040] The above carrier is air-dried, ground, passed through a 60-mesh sieve, and mixed with 50% of peat soil, 30% of activated carbon, 10% of sawdust, and 10% of rice husk.

[0041] (2) The preparation of mixed bacterial fermentation broth, see embodiment 1.

[0042] (3) The mixed bacterial fermentation liquid and the composite carrier are mixed evenly at a mass ratio of 0.5 to 1:1, and after ventilation and drying, the effective number of viable bacteria of the mixed bacteria is 10 8 -10 11 A / gram bacteria agent.

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Abstract

The invention provides a mixed bactericide for restoring places polluted by polynuclear aromatic hydrocarbons, a preparation method thereof, belonging to the field of environment pollution restoration engineering. The strains used by the bactericide are Bordetella, Ochrobactrum, Rhodococcus, Achcromobacter, Herbaspirillum and Microbacterium which are mixed according to the ratio of 21-101:40-200:22-102:20-100:1:2 for fermentation to obtain a bacterial liquid, and the bacterial liquid is mixed with solid carriers according to the mass ratio of 0.5-1:1 to obtain a solid bactericide which has the effective living bacteria count of 108-1011/g. The mixed bactericide in the invention can effectively remove polynuclear aromatic hydrocarbons polluting soils and water, and has the advantages of high activity, rapid growth and propagation, strong adaptability, no generation of secondary pollution and the like.

Description

technical field [0001] The invention relates to a mixed bacterial agent for controlling polycyclic aromatic hydrocarbons in the environment and an application method thereof, belonging to the field of bioremediation of organic polluted sites. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are persistent organic pollutants ubiquitous in the environment. Because of their carcinogenic, teratogenic, and mutagenic toxic effects, they are potentially dangerous to human health and the ecological environment. The U.S. Environmental Protection Agency and the European Community list it as a priority pollutant, and use 16 compounds of PAHs as monitoring parameters for environmental pollution. [0003] PAHs have extremely low water solubility, and it is difficult to remove them from the environment by conventional methods. Microbial remediation technology is considered to be one of the important means to degrade polycyclic aromatic hydrocarbons polluted environme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/00B09C1/10C02F3/34C12R1/025C12R1/01C02F101/32
Inventor 廖晓勇阎秀兰马栋
Owner INST OF GEOGRAPHICAL SCI & NATURAL RESOURCE RES CAS
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