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Extract from long pepper and preparation method and application thereof

A kind of extract, the technology of longi, applied to the extract from longi and the field of preparation and application, can solve the problems such as total alkaloid and piperine that have not been reported

Active Publication Date: 2011-09-21
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is no report in the literature on the related research of total alkaloids and piperine in Piper longum in the treatment of Parkinson's disease

Method used

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  • Extract from long pepper and preparation method and application thereof
  • Extract from long pepper and preparation method and application thereof
  • Extract from long pepper and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1, carry out active screening to three kinds of medicines (water extract of Piper longum, ethanol extract of Piper longum and piperine monomer)

[0047] 1. Preparation

[0048] The dried fruit of Piper longum in this example is a crude drug, which can be purchased from pharmacies.

[0049] Preparation of the water extract of Piper longum: Take the dried fruit of Piper longum, crush it into a coarse powder, extract three times with purified water 8 to 10 times the weight of the medicinal material, each time for 1 hour, filter, combine the filtrates, concentrate under reduced pressure, and make 2 g of crude drug / ml of Piper longum water extract.

[0050] The preparation of the alcoholic extract of Piper longum: Take the dried fruit of Piper longum, crush it into a coarse powder, extract three times with 95% ethanol solution 8 to 10 times the weight of the medicinal material, each time for 1 hour, filter, combine the filtrates, and depressurize at 60°C Recove...

Embodiment 2

[0066] Example 2. Activity screening for various elution sites of the ethanol extract of Piper longum

[0067] In order to further confirm the neuroprotective effect of the effective part of the alcohol extract of Piper longum, the cell viability was detected by MTT, and the effectiveness and concentration of various elution parts of the alcohol extract of Piper longum were screened.

[0068] 1. Preparation of various elution fractions

[0069] Piper longum extract A: take the dried fruit of Longan longan, crush it into a coarse powder, extract three times with 95% ethanol solution 8 to 10 times the weight of the medicinal material, each time for 1 hour, filter, combine the filtrates, and recover ethanol under reduced pressure at 60°C To alcohol-free, make the perennial alcohol extract of 2g crude drug / ml.

[0070] Piper longum extract B: take the dried fruit of Piper longum, crush it into a coarse powder, extract three times with purified water 8 to 10 times the weight of th...

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Abstract

The invention discloses an extract from long pepper and a preparation method and application thereof. The preparation method provided by the invention comprises the following steps: 1) extracting dry long pepper fruits by using 70 to 95 percent ethanol solution; 2) concentrating the extracted solution obtained in the step 1) to form concentrated solution with the relative density of 1 to 1.1; 3) diluting the concentrated solution obtained in the step 2) by using water, centrifuging the diluted solution, and adsorbing the centrifuged supernatant by using a D101 macroporous absorption resin column; and 4) eluting the D101 macroporous absorption resin column which is obtained in the step 3) and adsorbs the supernatant by using 20 percent ethanol, then eluting the D101 macroporous absorption resin column by using 70 percent ethanol of in a volume which is 20 times that of a column volume, and collecting the elution from the third to twentieth column volume so as to obtain the protected extract from the long pepper. In SH-SY5Y cells, SH-SK-N cells and MN9D cells, the extract of the invention has remarkable difference on nerve protection effect of oxidative damage caused by rotenone resistance compared with a rotenone model set, and has better medicinal effect than a positive medicinal set.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine, in particular to an extract from Piper longum and its preparation and application. Background technique [0002] Parkinson's disease (PD) is one of the most common neurodegenerative diseases clinically. With the aging of our population, the incidence of PD is on the rise. The US IMS Health Network reported that in 2004, the global expenditure on PD was 2.5 billion US dollars, and there are currently 3 million PD patients in China. The high disability rate after the onset of PD has become a major social problem affecting the health and quality of life of the elderly population in my country. The means of modern medicine to treat Parkinson's disease mainly include: drug therapy, surgery, cell transplantation, transgenic technology, etc. Due to the limitations of many factors, drug therapy is still the main method at present. Currently recognized as the best drug to control the clinica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/67A61P25/16A61K131/00
Inventor 杨慧吴霞罗容王年强毕赢
Owner CAPITAL UNIVERSITY OF MEDICAL SCIENCES