Compound amplification kit of 20 short tandem repeats

A technology of compound amplification and compound amplification system, which is applied to the detection of genetic marker genes with polymorphisms in the human genome and the simultaneous amplification of multiple short tandem repeat loci, can solve the problems of insufficient validity and DNA database data resources. waste, etc.
CN101818192BActive Publication Date: 2013-03-20BEIJING PEOPLESPOT TECH +1
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING PEOPLESPOT TECH
Publication Date
2013-03-20

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Abstract

The present invention provides a composite amplification system, for analyzing multiple STR loci at the same time, where it amplifies the 20 loci: Amelogenin, vWA, D21S11, D18S51, D5S818, D7S820, D13S317, D16S539, FGA, D2S1338, D19S433, D6S1043, D12S391, D8S1179, D3S1358, CSF1PO, Penta D, Penta E, TH01 and TPOX. Also provided are the methods and kits for analyzing DNA sample at the same time, as well as the uses thereof.
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Description

Technical field:

[0001] The present invention relates to the field of biotechnology. Further, the present invention relates to the detection of polymorphic genetic marker genes in the human genome. In particular, the present invention relates to the simultaneous amplification of multiple short tandem repeat loci in one amplification system using polymerase chain reaction. Background technique:

[0002] Short tandem repeats (STR for short), also known as microsatellites or simple sequence repeats (SSR), are a type of DNA tandem repeats widely present in eukaryotic genomes, with a core sequence of 2-6 base repeat units. The number of STR loci is large, the division is extensive, accounting for about 3% of the entire genome (International Human Genome Sequencing Consortium, 2001), and the polymorphism is high, and its polymorphism is mainly derived from the difference in the number of repeats of the core sequence between individuals. And this difference follows the Mendelian ...

Claims

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