Rapid detection method of blood pathogenic bacteria based on CE-SSCP (Capillary Electrophoresis-Single Strand Conformation Polymorphism)

A detection method and pathogenic bacteria technology, applied in the field of blood infection pathogenic bacteria detection, to achieve the effects of suitable amplification span, good applicability, and good repeatability
CN101824470BInactive Publication Date: 2013-06-19THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
Publication Date
2013-06-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a rapid detection method of blood pathogenic bacteria based on CE-SSCP (Capillary Electrophoresis-Single Strand Conformation Polymorphism). In the rapid detection method, specific to conserved regions of 16SrRNA genes of staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, staphylococcus epidermidis, klebsiella pneumoniae, enterococcus faecalis and streptococcus pneumoniae which are infected by blood, a corresponding PCR (Polymerase Chain Reaction) general primer is designed and subjected to fluorescence labeling, and then PCR is adopted to carry out amplification and electrophoresis detection on pathogenic bacteria in a blood sample, wherein the length of the designed general primer is suitable to the span of the amplification and has high complementary degree with the DNA of a pathogenic bacteria template in the blood sample to be tested, which enables an amplification product to have good specificity and favorable suitability to same kind of bacterium of different bacterial strains. The method has good repeatability and high sensitivity, can detect trace amount of the DNA of a pathogenic bacteria genome and is very suitable to wide application clinically.
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Description

technical field

[0001] The invention relates to a detection method for pathogenic bacteria infected with blood, in particular to a CE-SSCP-based rapid detection method for pathogenic bacteria in blood. Background technique

[0002] Blood infection often occurs in high-risk inpatients who are immunocompromised or undergoing invasive procedures. After infection, the symptoms of poisoning are severe and the mortality rate is high, which is very difficult in clinical work. Traditional detection methods for blood infection pathogenic bacteria mainly rely on biochemical identification after direct culture. This method requires separate cultivation and identification of each pathogen, which generally takes 3-5 days, which is time-consuming, and the test results are greatly affected by factors such as blood collection dose, time of inspection, and timing of medication.

[0003] Capillary electrophoresis (CE) is a new and efficient analysis and separation technology developed from t...

Claims

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