Constant temperature and humidity thin-layer chromatography tank

Abstract

The invention discloses a chromatography tank which comprises a temperature control device, a chromatography device contained in the temperature control device and a chromatography tank cover arranged on the chromatography device. The temperature control device controls the temperature of the chromatography device arranged in the temperature control device to enable the temperature in the saturating device to rapidly reach equalized temperature required by a test; the structure of the chromatography device can save solvent, is conveniently in presaturation, is convenient to be added with presaturant and developing solvent at any time and is convenient to control the relative humidity in the chromatography device; and the chromatography tank cover is used for sealing the chromatography tank device. the chromatography tank can save the solvent, is convenient in presaturation, is convenient to be added with the presaturant and the developing solvent at any time, is convenient to control the relative humidity and temperature in the saturating device, and has the advantages of low production and use cost, simple operation, easy control of test conditions and favorable chromatography repeated performance, provides reliable test conditions for establishing a thin-layer chromatography fingerprint and is convenient to record the test conditions.

Description

technical field [0001] The present invention relates generally to chemical analysis equipment, and in particular to thin layer chromatography cylinder equipment. Background technique [0002] Conventional thin-layer chromatography can detect multiple samples at the same time on the same plate, the analysis time is short, the stationary phase (adsorbent) is relatively cheap, and it is disposable. It is widely used in chemical analysis because of its strong intuition and the advantages of intuitive observation and comparison with the same plate. However, there are many factors that affect the analysis of thin-layer chromatography, resulting in poor repeatability and comparability of thin-layer chromatography, and it is difficult to meet the requirements of international and domestic interoperability. The main factors related to the chromatography tank are: [0003] 1. The effect of adsorbent activity and relative humidity on thin-layer chromatography [0004] Because TLC is...

AI Technical Summary

Problems solved by technology

Its shortcoming is that it is inconvenient to add developing agent. When adding developing agent, the chromatographic cylinder cover must be opened, which will cause the gas in the chromatographic cylinder to exchange with the outside gas and destroy various stable states in the chromatographic cylinder; It is easy to cause the developer to splash on the TLC plate, and it is easy to mix the sulfuric acid solution and the developer; if the experiment requires the developer and other solvents to pre-saturate the chromatography tank, there is no space for the existing chromatography tank to store other solvent

Its shortcomings are: the space of the condition control box is large, the experimental operation process cannot be operated under completely airtight conditions, and the experimental conditions controlled by the condition control box fluctuate greatly

Its disadvantages are: the relative humidity in the chromatography tank is different from that in the dryer, the test conditions in the chromatography tank have not been improved, and the TLC plates that have reached the experimental conditions in the dryer are not moving into the layer. After analysis in the cylinder, the activity of the thin-layer plate will change with the environment in the chromatography cylinder, that is, this method of controlling the humidity cannot effectively control the experimental conditions

[0011] 3. The effect of solvent vapor on thin layer chromatography

Its disadvantages are: the thin-layer plate does not participate in the pre-balance; when the thin-layer plate is put in, the chromatography cylinder cover must be opened, which will cause the gas in the chromatography cylinder to exchange with the outside gas, and destroy all kinds of gas in the chromatography cylinder. Steady state; no temperature and humidity control; inconvenient to add liquid

Its disadvantages are: it is difficult to control the flow of the developer in the chromatography tank into the side tank without solvent in the double tank chromatography tank; there is no temperature and humidity control; it is inconvenient to add liquid

Its shortcomings are: opening the chromatography cylinder cover is easy to destroy the environment in the chromatography cylinder that has reached the pre-saturation requirements; there is no temperature and humidity control; it is inconvenient to add liquid; the thin-layer plate does not participate in pre-saturation

Its shortcomings are: no temperature and humidity control; when the developing agent flows from a tank into the tank with the thin-layer board, it is easy to change the position of the thin-layer board, and at the same time, one end of the thin-layer board is first developed. , expand after the other end, affecting the unfolding effect

[0021] (2) The humidity in the chromatography tank cannot be effectively controlled in real time

[0022] (3) It is impossible to effectively control the "three-dimensional tomography" state in the chromatography cylinder during each experiment process in real time

[0023] (5) The temperature in the chromatography cylinder cannot be directly monitored

[0024] (6) In actual operation, it is easy to destroy the equilibrium state in the chromatography cylinder

[0025] (7) If the experiment requires both humidity control and developer and other solvents to pre-saturate the chromatography tank, there is no room for other solvents in the existing chromatography tank

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