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Anti-Cydia pomonella biological pesticide and preparation method thereof

A biopesticide, technology of codling moth, applied in botany equipment and methods, insecticides, biocides, etc., can solve the problems of low activity, low infection rate, single dosage form, etc.

Active Publication Date: 2012-07-25
安徽虫虫生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, both Bt and virus insecticides have shortcomings such as narrow insecticidal spectrum and single dosage form. These shortcomings have led to the fact that in my country, except for cotton bollworm NPV, which is used for field control every year, the application scale of other virus agents is very large. Small
[0004] Bt has low activity against some important agricultural pests such as codling moth, corn borer and rice leaf roller
For some viruses with a broad insecticidal spectrum, such as Autographa californica nuclear polyhedrosis virus (Autographa californica NPV, AcNPV) and cabbage armyworm nuclear polyhedrosis virus (Mamestra brassicae NPV, MbNPV), only a few kinds of insects have high insecticidal activity, although other insects can be infected, but the infection rate is low

Method used

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  • Anti-Cydia pomonella biological pesticide and preparation method thereof
  • Anti-Cydia pomonella biological pesticide and preparation method thereof
  • Anti-Cydia pomonella biological pesticide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098]A kind of anti-codling moth biopesticide, it is made of the raw material (%) of following percentage by weight: the preparation of codling moth granule virus suspension: CpGV final concentration is 30,000,000,000 granules / ml, and other compositions are made of The raw materials are as follows:

[0099]

[0100] A preparation method of a biopesticide against codling moth, the steps are as follows:

[0101] 1. Artificial rearing of codling moth

[0102] Composition and dosage of artificial feed for codling moth (improved according to Ivaldi-Sender, C. Ann. Zool. Ecol. Anim., 1974, 6:337-343): 800 ml of water, 20 grams of agar, 50 grams of corn flour, malt powder 50 grams, 50 grams of brewer's yeast, 6 grams of complex vitamins B and C, 2 grams of sodium benzoate, and 2 grams of ethyl paraben. When preparing, weigh 20 grams of agar and put it into a 1000 ml enamel measuring cup, add 800 ml of water, put it in a high-pressure sterilizer and sterilize it at 15 pounds fo...

Embodiment 2

[0120] A Bt C-33 bacterial strain resistant to codling moth is tested for its insecticidal activity against codling moth 3rd instar larvae by the following steps:

[0121] 1. Transfer the Bt C-33 strain to a petri dish containing beef extract peptone medium (beef extract 3 g / L, peptone 10 g / L, NaCl 5 g / L, agar powder 15 g / L, pH 7.0-7.5) , cultured upside down at 30°C for 72 h, and single colonies producing parasporal crystals were selected;

[0122] 2. Inoculate a single colony in liquid beef extract-peptone medium (beef extract 3 g / L, peptone 10 g / L, NaCl 5 g / L, pH 7.0-7.5), shake flask at 30°C, 200 rpm Medium fermentation until the parasporal crystals fall off, it is the fermentation liquid;

[0123] 3. The Bt C-33 toxin protein concentration was determined by the Coomassie Brilliant Blue G-250 method (Bradfold, Analyt Biochem., 1976, 72: 248-254), and the fermentation broth was diluted to 36.12, 18.06, 9.03, 4.52, 2.26 μg / ml 5 concentrations;

[0124] 4. The droplet ...

Embodiment 3

[0142] A kind of Bt against codling moth kurstaki standard strain, its insecticidal activity against codling moth 3rd instar larvae was tested by the following steps:

[0143] 1. Bt kurstaki The standard strain was transferred to a petri dish containing beef extract-peptone medium (beef extract 3 g / L, peptone 10 g / L, NaCl 5 g / L, agar powder 15 g / L, pH 7.0-7.5), at 30° C cultured upside down for 72 h, and single colonies producing parasporal crystals were selected;

[0144] 2. Inoculate a single colony in liquid beef extract-peptone medium (beef extract 3 g / L, peptone 10 g / L, NaCl 5 g / L, pH 7.0-7.5), shake flask at 30°C, 200 rpm Medium fermentation until the parasporal crystals fall off, it is the fermentation liquid;

[0145] 3. Determination of Bt by Coomassie Brilliant Blue G-250 kurstaki For standard toxin protein concentration, the fermentation broth was diluted to 5 concentrations of 26.46, 13.23, 6.62, 3.31, and 1.66 μg / ml by 2-fold dilution method;

[0146] 4...

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PUM

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Abstract

The invention discloses an anti-Cydia pomonella biological pesticide and a preparation method thereof. The preparation method comprises the following steps of: A, extracting Cydia pomonella granulosis viruses (CpGV) from dead Cydia pomonellas, artificially feeding Cydia pomonellas with modified artificial feed and proliferating the CpGV; B, diluting the proliferated CpGV to obtain suspension; C, screening Bt bacterial strains which have activity for Cydia pomonella larvae and centrifugally recovering thalli; D, adding light calcium carbonate, a pesticide emulsifier No.700, a penetrating agentJFC and table salt into the collected thalli and stirring uniformly; and E, preparing a Bt-CpGV mixture: uniformly mixing 24,000 IU / milligram Bt wettable powder and 3,000 million granules / milliliter CpGV suspension according to a volume ratio of 30-5:1, and drying the mixture to form compound wettable powder by freezing. The anti-Cydia pomonella biological pesticide has the advantages of reasonable formula, convenient use, practical method, simple and convenient operation, factory processing and preparing and high bacterial deinsectization speed.

Description

technical field [0001] The invention belongs to the technical field of green insecticide synergistic biological pesticides, more specifically relates to a biopesticide against codling moth, and also relates to a preparation method of a biopesticide against codling moth, i.e. Spore thuringiensis Bacillus (Bt) and codling moth granular virus (CpGV) compound biopesticide, the biopesticide can replace the chemical insecticides currently used in China to effectively control codling moth damage. Background technique [0002] codling moth ( Cydia pomonella L.) is a worldwide quarantine pest. Since it was discovered in Xinjiang in the late 1950s, it has become a pandemic in Xinjiang year after year. In recent years, the codling moth has damaged hundreds of thousands of hectares of fruit trees in Xinjiang every year, resulting in a 30-50% reduction in production and an annual loss of more than 3 billion yuan. Since the codling moth was introduced into Gansu Province in the mid-198...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00A01P7/04
Inventor 孙修炼刘向阳高梅影
Owner 安徽虫虫生物科技有限公司
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