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Method for improving chlorine resistance of cultivated soybeans by utilizing wild soybeans in intertidal zones

A technology for wild soybeans and cultivated soybeans, applied in horticultural methods, plant genetic improvement, botanical equipment and methods, etc., can solve problems such as limited salt tolerance, narrow genetic basis, low yield and economic value of wild soybeans, and achieve grain yield The effect of quality improvement

Inactive Publication Date: 2011-03-02
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the narrower genetic basis of cultivated soybeans and relatively limited salt tolerance, it is difficult to make major breakthroughs in the breeding work limited to the range of cultivated soybean varieties, while the yield and economic value of wild soybeans are relatively low

Method used

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  • Method for improving chlorine resistance of cultivated soybeans by utilizing wild soybeans in intertidal zones
  • Method for improving chlorine resistance of cultivated soybeans by utilizing wild soybeans in intertidal zones
  • Method for improving chlorine resistance of cultivated soybeans by utilizing wild soybeans in intertidal zones

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Experimental program
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Effect test

Embodiment 1

[0027] A method for improving the chlorine resistance of cultivated soybeans by using tidal flat wild soybeans, comprising the following steps:

[0028] (1) Select cultivated soybeans and wild soybeans for diallel hybridization, take the cultivated soybean Jackson as the female parent, cross with the male parent wild soybean BB52 after emasculation, and harvest hybrid F 1 substitute seeds;

[0029] (2) Field planting of F for several consecutive years 1 Generation seeds received to F 4 Generation, F 1 to F 4 The seedlings of each generation were treated with gradually increasing concentrations of salt, and the surviving soybean seedlings were desalted and cultivated until they matured and podded, and the seeds (such as 185 strains) were harvested. f 1 to F 4 The method that the seedlings of each generation were treated with salt with gradually increasing concentration was as follows: after emergence, the seedlings were treated with 50, 100, 150 and 200 mmol / L NaCl fo...

Embodiment 2 and Embodiment 3

[0032] Embodiment 2 and embodiment 3 methods are the same as embodiment 1, and the female parent, male parent, hybridization mode, backcrossing mode and result details that each embodiment adopts are shown in Table 1.

[0033]

[0034] Table 1 The detailed table of hybridization mode and test result of embodiment 2 and 3

[0035]

[0036] The above-mentioned embodiments are arbitrarily selected, and various indicators are tested on the growth process and the harvested seeds.

Embodiment 4

[0037] Embodiment 4: investigation of agronomic traits

[0038] Cultivated soybeans and wild soybeans of the tested parents and their F 4 The first-generation strains were sown in the field of the experimental farm in late May, and were routinely managed until November of the same year. The seeds were harvested after the seeds were fully ripe, and the plant height, number of pods per plant, number of branches, number of stem nodes, number of pods per plant and hundred Field agronomic indicators such as grain weight, and stored grain (F 5 generation). Such as image 3 and Figure 4 It was shown that the agronomic traits of pod and seed appearance, plant height, branch number and other agronomic traits of the hybrid progenies screened by hybridization and chlorine resistance were basically between the parents, but they were biased towards cultivated soybeans.

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Abstract

The invention discloses a method for improving the chlorine resistance of cultivated soybeans by utilizing wild soybeans in intertidal zones, comprising the following steps: carrying out diallel cross on a cultivated soybean variety and a wild soybean metapopulation; harvesting F1 generation hybrid seeds; cultivating in a farmland for years until harvesting F4 generation hybrid seeds; processing F1-F4 generation seedlings respectively by utilizing salts with gradually incremental concentrations; carrying out desalination cultivation on the survival seedlings until the seedlings are mature and bear pods; and then carrying out backcross transformation on the mature seedlings and a high-class cultivated soybean variety. The method disclosed by the invention has less steps and is simple in operation; and through repeating experiments, the agronomic characteristics, such as appearances of seed pods and seeds, plant height, winding number and the like, of the filial generations obtained by hybridization and chlorine resistance generational screening are fundamentally between those of parents, and the chlorine resistances of the filial generations are superior to those of the parents and are obviously improved compared with the female parent (the cultivated soybean variety).

Description

technical field [0001] The invention belongs to the technical field of new germplasm of stress-tolerant crops, and in particular relates to a method for improving the chlorine resistance of cultivated soybeans by using wild soybeans in tidal flats. Background technique [0002] According to reports, the world's saline soil accounts for about 7% of the total land area of ​​the earth, and about 22% of the agricultural land is being affected by increasing salt damage. The area of ​​saline land in my country is about 36 million hectares. As far as Jiangsu Province is concerned, there are more than 711 kilometers of coastline, and the area of ​​tidal flat soil above the zero meter line is 6.1382 million mu, accounting for about 4.04% of the total land area of ​​Jiangsu Province. Therefore, the improvement of crop salt tolerance and quality, biological treatment and comprehensive development of saline soil have become major issues for future agricultural development. The loss of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01G7/06
Inventor 於丙军罗庆云刘友良章文华陈宣钦杜莉莉张晓可张艳周其好
Owner NANJING AGRICULTURAL UNIVERSITY
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