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Process to diagnose or treat brain injury

A technology of brain injury and treatment, which is used in the field of diagnosis or treatment of brain injury, and can solve problems such as brain injury that is difficult to treat effectively

Inactive Publication Date: 2011-03-02
BANYAN BIOMARKERS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Furthermore, brain injuries are often difficult to treat effectively, and successful outcomes often depend on how quickly an individual is diagnosed with a specific injury subtype

Method used

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  • Process to diagnose or treat brain injury
  • Process to diagnose or treat brain injury
  • Process to diagnose or treat brain injury

Examples

Experimental program
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example 1

[0094] Example 1: Materials for biomarker analysis. Sodium Bicarbonate, (Sigma Cat#: C-3041), Blocking Buffer (Startingblock T20-TBS) (Pierce Cat#: 37543), Tris Salt Buffer with Tween 20 (TBST; Sigma Cat#: T-9039 ). Phosphate buffered saline (PBS; Sigma Cat#: P-3813); Tween 20 (Sigma Cat#: P5927); Super TMB ELISA (Pierce Cat#: 34028); and Nunc maxisorp ELISA plates (Fisher). Monoclonal and polyclonal UCH-L1 antibodies were self-made or obtained from Santa Cruz Biotechnology, Santa Cruz, CA. Antibodies for alpha-II spectrin and degradation products and MAP2 are available from Santa Cruz Biotechnology, Santa Cruz, CA. Labels for antibodies of many subtypes are available from Invitrogen, Corp., Carlsbad, CA. Protein concentrations in biological samples were determined with standard albumin using the bicinchoninic acid microprotein assay (Pierce Inc., Rockford, IL, USA). All other necessary reagents and materials are known and readily available to those skilled in the art.

example 2

[0095] Example 2: In vivo model of TBI injury model: A controlled cortical impact (CCI) device was used to prepare a rat TBI model as previously described (Pike et al, 1998). Adult male (280-300g) SD rats (Sprague-Dawley rats) (Harlan: Indianapolis, IN) were used at 1:1 O 2 / N 2 Anesthetize with 4% isoflurane in O carrier gas (4 min), then maintain in 2.5% isoflurane in the same carrier gas. Core body temperature was continuously monitored with a rectal thermistor probe and maintained at 37±1°C by placing an adjustable temperature-controlled heating pad underneath the rat. Animals were fixed on a stereotaxic frame in a prone position and secured with ear and incisor rods. A unilateral (ipsilateral to the impingement site) craniotomy (7 mm in diameter) was performed along the midline incision and soft-tissue reflection, adjacent to the midline, between the bregma and lambda. The dura mater remains intact over the cortex. Brain injury was produced by impacting the right (ips...

example 3

[0096] Example 3: Middle cerebral artery occlusion (MCAO) injury model: Rats were cultured under isoflurane anesthesia (5% isoflurane via inspiratory chamber, then 2% isoflurane via nose cone). The arterial artery (CCA) was exposed by midline dissection of the neck at the bifurcation of the external carotid artery (ECA) and internal carotid artery (EIA). The ICA is located rostrally posterior to the pterygopalatine branch, and the ECA is ligated and severed at its lingual and maxillary bifurcation. A 3-0 nylon suture is then introduced into the ECA through an incision over the ECA stump (the path of the suture is monitored visually through the vessel wall) and advanced through the carotid canal to approximately 20 mm from the carotid bifurcation until it rests anterior to the brain A narrowing of the artery blocks the source of the middle cerebral artery. The skin incision is then closed and the endovascular sutures left in place for 30 minutes or 2 hours. The rat is then br...

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Abstract

A process for diagnosing and treating a neurological condition in a subject is provided that includes assaying a biological sample of a subject for the presence of one or more biomarkers; diagnosing a neurological condition based on a ratio of one or more of the biomarkers in the sample; and administering a therapeutic to the subject to alter the ratio of one or more biomarkers. The diagnosis numerous neurological conditions such as brain injury, or multiple organ injury is provided.

Description

[0001] Cross References to Related Applications [0002] This application claims U.S. Provisional Application No. 61 / 063,515, filed February 4, 2008; U.S. Provisional Application No. 61 / 055,737, filed May 23, 2008; and 61 / 085,623, filed August 1, 2008 Priority of U.S. Provisional Application No. The contents of each provisional application are hereby incorporated by reference as if each provisional application were expressly and completely set forth herein. technical field [0003] The present invention generally relates to methods for diagnosing or treating injuries. The method of the present invention allows simultaneous diagnosis and treatment of lesions. Diagnosis and treatment of Traumatic brain injury in individuals alone or involving multi-organ trauma. Background technique [0004] Traumatic brain injury (TBI) is the leading cause of death and disability for persons under the age of 45 in industrialized countries (McAllister, 1992). Of the estimated 1.5 million h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N2800/28G01N2800/2871G01N33/6896G01N2333/914A61P9/10A61P25/00A61P43/00
Inventor 王凯文罗纳德·L·海斯
Owner BANYAN BIOMARKERS INC
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