Unlock instant, AI-driven research and patent intelligence for your innovation.

Composite culture medium for algae-killing bacterium and preparation method thereof

A culture medium and algicidal technology, applied in the field of culture medium

Inactive Publication Date: 2012-08-22
XIAMEN UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic research on algicides is still in its infancy, but it has become a new idea to develop algicides by screening algicides that are efficient, specific, and biodegradable.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composite culture medium for algae-killing bacterium and preparation method thereof
  • Composite culture medium for algae-killing bacterium and preparation method thereof
  • Composite culture medium for algae-killing bacterium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] The composition of the compound culture medium of described algicide is, contains in the old seawater of 1L:

[0067] Tryptone 14g;

[0068] Yeast powder 1.63g;

[0069] Soluble starch 5.0g;

[0070] NaNO 3 1.6g;

[0071] MgSO 4 2.3g;

[0072] Adjust the pH to 7, the salinity of the old seawater is 30‰, and its preparation method is: tryptone, yeast powder, soluble starch, NaNO 3 and MgSO 4 Add old sea water to make up the volume, adjust the pH to 7, and autoclave at 121°C for 20min. That is, the compound culture medium for algicidal bacteria is obtained.

[0073] Through the above-mentioned optimization experiments, the optimal medium formula and optimal culture conditions of the algicidal bacteria DH46 were obtained. The initial medium and the optimized medium were used for the shake flask culture of DH46 respectively, and the optimized culture conditions were adopted as the culture conditions. The optimized bacterial cell fermentation has a si...

Embodiment 2

[0077] Similar to Example 1, the difference is that the composition of the culture medium contains:

[0078] Tryptone 1g;

[0079] Yeast powder 1g;

[0080] Soluble starch 0.5g;

[0081] NaNO 3 1g;

[0082] MgSO 4 0.1g;

[0083] The pH is adjusted to be 7.5, and the salinity of the aged seawater is 20‰.

Embodiment 3

[0085] Similar to Example 1, the difference is that the composition of the culture medium contains:

[0086] Tryptone 15g;

[0087] Yeast powder 5g;

[0088] Soluble starch 5g;

[0089] NaNO 3 3g;

[0090] MgSO 4 3g;

[0091] The pH is adjusted to be 7.8, and the salinity of the aged seawater is 40‰.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a composite culture medium for an algae-killing bacterium and a preparation method thereof, relates to a culture medium, and provides a composite culture medium suitable for culturing the algae-killing bacterium Alteromonas addita DH46 and capable of obviously improving the cell density of the algae-killing bacterium, and a preparation method thereof. The composite culturemedium comprises the following components, namely 1L of aged seawater contains 1 to 15g of tryptone, 0.5 to 5g of yeast powder, 0.5 to 5g of soluble starch, 0.1 to 3g of NaNO3, and 0.1 to 3g of MgSO4, wherein the pH is 7.2 to 7.8, and the salinity of the aged seawater is 2 to 4 percent. The preparation method comprises the following steps of: adding the tryptone, the yeast powder, the soluble starch, the NaNO3, and the MgSO4 into the aged seawater for fixing the volume, regulating the pH to be 7.2-7.8, and sterilizing to obtain the composite culture medium for the algae-killing bacterium.

Description

technical field [0001] The invention relates to a culture medium, in particular to a compound culture medium of Alteromonas addita DH46 and a preparation method thereof. Background technique [0002] With the aggravation of eutrophication in global water bodies, the frequency, scale and harm of harmful red tides have increased greatly, causing serious ecological, resource and environmental problems and major economic losses. At present, because red tides have become a worldwide public hazard, red tides occur frequently in more than 30 countries and regions, including the United States, Japan, China, Canada, France, Sweden, Norway, the Philippines, India, Indonesia, Malaysia, South Korea, and Hong Kong. Therefore, it has become an urgent task for many countries and regions to study the prevention and control methods of red tide and formulate relevant countermeasures. Marine phytoplankton is the main organism that causes red tides. Among the more than 4,000 species of marine ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 郑天凌林婧田蕴周艳艳郑小伟周月霞杨小茹苏建强张帮周
Owner XIAMEN UNIV