Stagnation-eliminating chromatographic column, stagnation-eliminating chromatograph and analysis method thereof

Abstract

The invention provides a chromatographic column capable of eliminating a stagnation layer on the surfaces of adsorbent particles and having high column performance, a chromatograph and an analysis method thereof to solve the technical problems. The stagnation-eliminating chromatographic column comprises a column pipe and an adsorbent filled inside a cavity to serve as a stationary phase, wherein the column pipe is made of a non-conductive insulating material, or the column pipe is made of a metal material but a non-conductive insulating material is arranged on an inner wall of the metal column pipe. Because a central electrode is absent in the chromatographic column, the conventional voltage application along the radial direction of the chromatographic column is changed into axial voltage application, the chromatographic column is uniformly heated, the temperature difference between the center and the edge of the chromatographic column is low, and the column performance of the chromatographic column can be obviously improved. Therefore, the chromatograph has higher separation capacity and higher analysis speed.

Description

technical field [0001] The invention relates to a liquid chromatography analyzer, in particular to a stagnation chromatography column, a stagnation chromatography instrument and an analysis method thereof. Background technique [0002] The liquid chromatography analyzer is mainly composed of chromatographic column, sampling system, infusion pump, detector, data system and other parts. The chromatographic column includes a column tube and an adsorbent filled in its inner cavity as a stationary phase. When using a liquid chromatographic analyzer, the sample is brought into the chromatographic column by the mobile phase. Since the adsorption capacity of different substances in the tested sample is different from the stationary phase, different substances leave the chromatographic column in the order of adsorption capacity to reach the detection. Detector, the detector detects the signal of the substance being eluted, and compares these signals to carry out qualitative and quan...

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Problems solved by technology

But because there is a central electrode in this method, when working, the current density in the middle of the chromatographic column is high, the temperature is high, and there is a large temperature difference between the middle and the edge of the column tube, and the column efficiency of the chromatographic column will be affected, thereby affecting the performance of the liquid chromatograph. Analytical performance

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