Rosmarinic acid, rosmarinic acid-containing common selfheal fruit-spike active ingredient and preparation methods and application thereof to prevention and treatment of cancer postoperative metastasis

A technology of rosmarinic acid and effective components, which is used in the application field of preparing medicines for preventing or treating postoperative metastasis of cancer

Active Publication Date: 2011-04-20
SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there are no reports about the pharmacological effects of rosmarinic acid or compositions containing rosmarinic acid in treating or preventing postoperative metastasis of cancer

Method used

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  • Rosmarinic acid, rosmarinic acid-containing common selfheal fruit-spike active ingredient and preparation methods and application thereof to prevention and treatment of cancer postoperative metastasis
  • Rosmarinic acid, rosmarinic acid-containing common selfheal fruit-spike active ingredient and preparation methods and application thereof to prevention and treatment of cancer postoperative metastasis
  • Rosmarinic acid, rosmarinic acid-containing common selfheal fruit-spike active ingredient and preparation methods and application thereof to prevention and treatment of cancer postoperative metastasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1 Preparation of Prunella vulgaris Active Components Containing Rosmarinic Acid

[0057] Take 3kg of Prunella vulgaris, add 16 times of water and decoct three times, the first time is 1.5h, the second time is 1h, the third time is 0.5h, the three decoctions are combined, filtered, concentrated to 3000ml, and passed through the D101 macroporous adsorption resin column, elute with 7 column volumes of 70% ethanol to FeCl 3 -K 3 Fe(CN) 6 The reaction was negative, and the combined eluents were concentrated to contain 4 g of crude drug per milliliter to obtain the Prunella vulgaris extract, which was extracted 5 times with petroleum ether and ethyl acetate successively (extract solution: extraction solvent = 2: 1), and discarded. In addition to the extract, the aqueous solution is then extracted with water-saturated n-butanol, and the n-butanol is recovered to obtain the active site solution of Prunella vulgaris, and the solution is spotted on a 20cm×20cm silica...

Embodiment 2

[0059] Embodiment 2 Preparation of Prunella vulgaris Active Components Containing Rosmarinic Acid

[0060] Take 5kg of Prunella vulgaris, add 14 times of water to decoct twice, the first time for 1h, the second time for 0.5h, combine the two decoctions, filter, concentrate to 1.06 (60°C), put it to room temperature, add ethanol, and make The alcohol content is 70%, refrigerate for more than 48 hours, recover the ethanol until the relative density of the solution is 1.28 (80°C), put it at room temperature, then adjust the alcohol content to 85% with ethanol, refrigerate for more than 48 hours, and concentrate the supernatant to 1.27 (80°C), adjust the pH to 2 with concentrated hydrochloric acid, refrigerate for more than 24 hours, filter, and adjust the pH of the filtrate to 7 with 40% sodium hydroxide solution to obtain the extract of Prunella vulgaris. The extract was extracted 5 times with petroleum ether and ethyl acetate successively (extract: extraction solvent = 3: 1), t...

Embodiment 3

[0061] Embodiment 3 Preparation of Prunella vulgaris Active Components Containing Rosmarinic Acid

[0062] Take 1 kg of Prunella vulgaris, add 12 times of water to decoct four times, the first two times for 1 hour each, and the second time for 0.5 hours each, combine the decoction, filter, concentrate to 6000ml, add 300ml of 1% chitosan acetic acid solution, shake well , let it stand for 12 hours, centrifuge at high speed (4000rpm, centrifuge for 10min), take the supernatant, concentrate to contain 4g of crude drug per milliliter, adjust the pH to 2 with concentrated hydrochloric acid, refrigerate for more than 24 hours, filter, and the filtrate is 40% sodium hydroxide The pH of the solution was adjusted to 7 to obtain the extract of Prunella vulgaris. The extract was sequentially extracted 5 times with petroleum ether and ethyl acetate respectively (extract: extraction solvent = 3:1), the extract was discarded, the aqueous solution was extracted with saturated n-butanol, and ...

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Abstract

The invention discloses rosmarinic acid, a rosmarinic acid-containing common selfheal fruit-spike active ingredient and preparation methods thereof by extraction and separation. Moreover, the invention also discloses the application of the rosmarinic acid or/and the rosmarinic acid-containing common selfheal fruit-spike active ingredient to the preparation of a medicament for preventing or treating cancer postoperative metastasis and discloses a medicinal composition which contains effective doses of the rosmarinic acid or/and the rosmarinic acid-containing common selfheal fruit-spike active ingredient for preventing or treating the cancer postoperative metastasis. The rosmarinic acid and the rosmarinic acid-containing common selfheal fruit-spike active ingredient are obtained by extraction and separation. Pharmacodynamic experiment researches indicate that the rosmarinic acid and the rosmarinic acid-containing common selfheal fruit-spike active ingredient prepared by the method have cancer metastasis resistance and can be used for preparing a medicament for preventing or treating cancer metastasis.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and in particular relates to rosmarinic acid, effective components of Prunella vulgaris containing rosmarinic acid, an extraction and separation method thereof, and an application thereof in preparing medicines for preventing or treating postoperative metastasis of cancer. Background technique [0002] The chemical name of rosmarinic acid (RosA) is R(+)2-((3-(3,4-dihydroxyphenyl-1-oxo-2propenyl)oxy)3,4 -Dihydroxyphenylpropionic acid}, is a water-soluble polyphenolic compound, widely distributed in plants, mainly in various species of Labiatae, Boraginaceae, Cucurbitaceae, Tiliaceae, Umbelliferae Among plants, Chinese patent application publication CN1995007A (application number 200510131129.7, publication date: July 11, 2007) discloses a method for preparing rosmarinic acid from medicinal materials containing rosmarinic acid. [0003] Rosmarinic acid has antibacterial, anti-inflammato...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/536A61K31/216A61P35/04C07C69/732
CPCC07C67/48A61K36/536A61P35/04C07C69/732
Inventor 刘力徐德生
Owner SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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