Enterobacter aerogenes M6R9 capable of degrading pyrethroid pesticide residues

A technology of Enterobacter aerogenes and colony characteristics, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve the problem of less microorganisms, etc., and achieve the effect of broad application prospects.

Inactive Publication Date: 2011-04-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most microorganisms degrade one or a few pesticides, and there are few re...

Method used

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  • Enterobacter aerogenes M6R9 capable of degrading pyrethroid pesticide residues
  • Enterobacter aerogenes M6R9 capable of degrading pyrethroid pesticide residues
  • Enterobacter aerogenes M6R9 capable of degrading pyrethroid pesticide residues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1: Screening and identification of degrading strains

[0051] 1 Materials and methods

[0052] 1.1 Medium and reagents

[0053] Enrichment medium: peptone 10g, NaCl 1.0g, KH 2 PO 4 1.0g, H 2 O 1000mL, glucose 1.0g, pH 7.0;

[0054] Basal medium: NH 4 NO 3 1.00g, MgSO 4 .7H 2 O 0.5g, (NH 4 ) 2 SO 4 0.5g, KH 2 PO 4 0.5g, NaCl0.5g, K 2 HPO 4 1.5g, H 2 O1000mL, pH7.0;

[0055] Add 1.5% (w / v, 1.5g per 100mL) agar and 100mg.L -1 Bifenthrin, fenpropathrin and cypermethrin can be formulated into corresponding pesticide-containing solid medium;

[0056] 50g·L -1 bifenthrin, fenpropathrin and cypermethrin emulsion.

[0057] 1.2 Determination of strains on the degradation efficiency of bifenthrin, fenpropathrin and cypermethrin

[0058] The purified single strain was measured as OD 415nm =0.2 Inoculate into 100mL containing 100mg·L of bifenthrin, fenpropathrin and cypermethrin -1 In a 250mL Erlenmeyer flask of sterile liquid enrichment medium, use ...

Embodiment 2

[0091] Example 2: Degradation Performance Detection of Bacterial Strains

[0092] 1 Materials and methods

[0093] 1.1 Medium and reagents

[0094] Basal medium: NH 4 NO 3 1.00g, MgSO 4 .7H 2 O 0.5g, (NH 4 ) 2 SO 4 0.5g, KH 2 PO 4 0.5g, NaCl0.5g, K 2 HPO 4 1.5g, H 2 O1000mL, pH7.0;

[0095] 50g·L -1 bifenthrin, fenpropathrin and cypermethrin emulsion.

[0096] 1.2 Degradation of strains to bifenthrin, fenpropathrin and cypermethrin

[0097] In a Erlenmeyer flask containing 100 mL of basal medium, mix and add 100 mg·L -1 Bifenthrin, fenpropathrin, and cypermethrin were used as the only carbon source, at the inoculation amount OD 415nm =0.2, 25~30℃, pH 7.0, shaking speed 180r·min -1 100mL (250mL Erlenmeyer Flask) condition of liquid filling capacity after cultivating for 3 days, measure the degradation rate of bacterial strain M6R9 to 3 kinds of pyrethroid pesticides according to the method of Example 1.

[0098] 2. Results

[0099] Strain M6R9 was tested ...

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Abstract

The invention provides Enterobacter aerogenes M6R9 which can degrade pyrethroid pesticide residues, in particular can degrade the residues of three pyrethroid pesticides at the same time, namely bifenthrin, fenpropathrin and cypermethrin. The strain is preserved in the China General Microbiological Culture Collection Center (address: Institute of Microbiology of Chinese Academy of Sciences, Building 3, No. 1 Precincts, Beichen West Road, Chaoyang District, Beijing, 100101), the preservation number of the strain is CGMCC No. 4155, and the conservation date is September 8, 2010. The beneficial effects of the Enterobacter aerogenes M6R9 in the invention are mainly embodied as follows: the invention provides a new strain which can degrade the residues of bifenthrin, fenpropathrin and cypermethrin at the same time and provides the research basis for controlling the pyrethroid pesticide residues in agricultural products, and the strain has wide application prospect.

Description

(1) Technical field [0001] The invention relates to a strain of Enterobacter aerogenes M6R9 capable of degrading pyrethroid pesticide residues, especially three pyrethroid pesticide residues of bifenthrin, fenpropathrin and cypermethrin simultaneously. (2) Background technology [0002] Pyrethroid pesticides are synthesized by simulating natural pyrethrins and contain multiple benzene ring structures. They have the characteristics of broad-spectrum, high efficiency, low toxicity, resistance to light and heat, and their number of varieties and usage are second only to Organophosphorus pesticides, which occupy the second place in the insecticide market, are widely used in the pest control of economic crops such as tea and vegetables. Bifenthrin, fenpropathrin, and cypermethrin are commonly used. However, this kind of pesticide generally has the characteristics of stable environment, slow degradation rate, low degradation rate, and high residue, which brings food safety problem...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/04A62D101/28
Inventor 廖敏谢晓梅
Owner ZHEJIANG UNIV
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