Cypermethrin degrading bacteria in marine environment, and separation, purification and application thereof

A technology of cypermethrin degradation and cypermethrin, applied in chemical instruments and methods, biochemical equipment and methods, bacteria, etc., can solve environmental, food safety and human health hazards, fishery production and ecological environment impacts, etc., to eliminate biological toxicity , broad development prospects, good degradation effect

Active Publication Date: 2013-01-30
路亚科消防车辆制造有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the residual cypermethrin can enter the water body through surface runoff or the use of aquaculture sea areas, which wil

Method used

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  • Cypermethrin degrading bacteria in marine environment, and separation, purification and application thereof
  • Cypermethrin degrading bacteria in marine environment, and separation, purification and application thereof

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0022] Screening and Identification of Degrading Strains

[0023] 1. Media and reagents

[0024] Nutrient medium: Accurately weigh 0.01 g of ferric phosphate, 5.0 g of peptone, and 1.0 g of yeast extract, add 1,000 mL of filtered seawater, mix well, adjust the pH to 7.0-7.8, and sterilize with high pressure steam (121°C, 20 min) be made of.

[0025] Enriched culture medium: Add cypermethrin solution to the nutrient medium so that the concentration of cypermethrin is 100 mg / L.

[0026] 2. Isolation and purification of bacterial strains:

[0027] Seawater samples were collected from the coastal waters of Ningbo, and 500mL of seawater was taken to be filtered and enriched in a sterile state, then added to the enrichment medium containing 100 mg / L cypermethrin, and cultured at a temperature of 28°C and a rotational speed of 180 rpm. 7d, repeat the above operation process 3 times, the inoculum of each culture is taken from the culture solution obtained in the previous time, aft...

specific Embodiment 2

[0029] Identification of Beta-Cypermethrin Pesticide Degrading Strains

[0030] The bacterial strain obtained above is carried out morphological characteristics and molecular biological identification, and the electron micrograph of this bacterial strain is as follows: figure 1 shown. The main biological characteristics of the strain are: Gram negative, bacillus, β-galactosidase, arginine double hydrolysis test, citrate test, lysine decarboxylase, ornithine decarboxylation test, V-P reaction , gelatin liquefaction test positive, urease test, H 2 S reaction, tryptophan decarboxylase, indolease test negative, can not use sucrose. The strain grows in seawater, the optimum growth temperature is 25-30°C, and the optimum growth pH is 7.0-7.8. On solid medium, the colony is round, thin, white and translucent. The strain was analyzed by 16s rRNA sequence It was identified as Mesonia sp.

specific Embodiment 3

[0031] Degradation effect and application of cypermethrin-degrading bacteria on pyrethroid pesticides in seawater

[0032] Inoculate the isolated and purified strains into 100 mL of enrichment medium containing 100 mg / L cypermethrin (the preparation method is as shown in Example 1), and use the enrichment medium without inoculation as a control, at 28°C, The culture was shaken on a constant temperature shaker at 180 rpm for 7 days. After culturing, draw 2 mL of culture solution, add 0.4 g NacL, vortex and mix well, and use 4 mL of a mixed solution of n-hexane and acetone (volume ratio 1:1) and 2 mL of a mixed solution of n-hexane and acetone Extract, combine the supernatant obtained from two extractions, take 3 mL supernatant, add 0.4 g anhydrous sodium sulfate, blow dry with nitrogen, and dissolve it with a mixed solution of 1 mL isooctane and acetone (volume ratio 1:1), For GC-ECD analysis and detection, each experiment was repeated three times, and the degradation ability ...

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Abstract

The invention discloses a cypermethrin degrading bacteria in marine environment, wherein the bacteria is an HS-12 strain, with a classification naming as Mesoniasp., and is preserved in China General Microbiological Culture Collection Center on April 24, 2012, an accession number being CGMCCNo. 6045; and a Genbank accession number of the 16SrRNA of the strain is JX087927. The invention simultaneously discloses a separation and purification method and an application of the cypermethrin degrading bacteria in the marine environment. The invention is advantageous in that the bacteria can safely, highly-efficiently and rapidly degrade the cypermethrin in the marine environment, and has a good application prospect in the aspects of offshore environmental protection, pollution treatment of pesticide residues in an aquaculture environment and food safety guarantee.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a cypermethrin-degrading bacterium in a seawater environment and its separation, purification and application. Background technique [0002] Cypermethrin contains multiple benzene ring structures, is insoluble in water, and is a highly lipophilic pesticide. It is a highly efficient and broad-spectrum synthetic pyrethroid insecticide. It is currently widely used in orchards, tea gardens, farmland, and fish farming. Prevention and treatment. However, the residual cypermethrin can enter the water body through surface runoff or use in aquaculture sea areas, which brings great harm to the environment, food safety and human health, and affects fishery production and the ecological environment. [0003] At present, the restoration methods for pesticide residues mainly include physical, chemical and biological restoration methods. Compared with physical and chemical methods with hig...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10A62D3/02C12R1/01C02F101/38A62D101/04A62D101/28
Inventor 刘菁华史西志孙爱丽李德祥周磊陈炯
Owner 路亚科消防车辆制造有限公司
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