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Application of rice gene KT506 in improving stress tolerance of plant

A kind of plant stress resistance and plant technology, which is applied in the application field of improving plant salt tolerance, can solve the problems of improvement, difficult crop traits, and inability to maintain excellent agronomic traits, and achieve broad application prospects and the effect of improving tolerance

Active Publication Date: 2011-04-20
BEIJING WEIMING KAITUO CROP DESIGN CENT COMPANYLIMITED
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Problems solved by technology

However, some genes with definite functions obtained by means of gene deletion mutants, especially genes related to stress resistance, are difficult to be applied to the improvement of crop traits. The main reason is that while improving the stress resistance performance, it also affects Normal growth of plants, unable to maintain good agronomic traits

Method used

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  • Application of rice gene KT506 in improving stress tolerance of plant
  • Application of rice gene KT506 in improving stress tolerance of plant
  • Application of rice gene KT506 in improving stress tolerance of plant

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Embodiment Construction

[0025] The methods used in the following examples are conventional methods unless otherwise specified, and the specific steps can be found in Molecular Cloning. The primers and DNA sequences used were synthesized by Shanghai Yingjun Biotechnology Co., Ltd., and the sequencing was completed by Beijing Huada Gene Research Center. Nucleic acid restriction endonuclease and T4 DNA ligase in the process of vector construction were purchased from NEB Company. Methods All were carried out according to the method provided in the kit. The vector backbone used in the experiments was from pCAMBIA1300.

[0026] 1. Isolation of KT506 gene

[0027] Infer the coding sequence of the KT506 gene, design the 5' end primer from the coding start site ATG of the gene, and design the 3' end primer at the stop codon:

[0028] Primer 1: 5'GCACGCtctagaATGGATCGGCATGAGGAGGAG 3'

[0029] Primer 2: 5'GCACGCctgcagTCAGTCTCGGTCATCGATGAAG 3'

[0030] The tctaga sequence in primer 1 is the restriction endonu...

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Abstract

The invention discloses a gene KT506 which is derived from rice and relates to the stress resistance. Experiments show that through the application of the rice gene KT506, the tolerance of rice (subjected to gene transformation) to high salt can significantly be improved. In the application of the rice gene KT506, proteins and coding genes of the proteins have important theoretical and practical significances to the study on the stress resistance mechanism of the plant and the improvement on the stress resistance and correlated characters of the plant, and play an important role in the stresstolerant genetic engineering improvement of the plants (in particular to cereal crops), thus the prospect of application is broad.

Description

technical field [0001] The invention relates to a gene related to plant stress tolerance and its application, in particular to the application of KT506, a gene related to stress resistance derived from rice, in improving plant salt tolerance. Background technique [0002] Rice, corn and wheat are important food crops in my country. The yield and quality of the three major crops are crucial to my country's food production and food security. Abiotic stresses such as drought, salinity, high temperature and freezing damage will directly affect the normal growth and yield of food crops. [0003] With the rapid development of molecular biology technology and the application and popularization of transgenic technology, more and more attention has been paid to the cultivation of new varieties of transgenic organisms whose main goal is to improve crop traits. The governments of the United States, Japan, Canada, South Korea, and some European countries have invested considerable fund...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/63C12N15/82C07K14/415A01H4/00A01H5/00
Inventor 刘雨刘春霞李晓娟李早霞王喜萍周君莉
Owner BEIJING WEIMING KAITUO CROP DESIGN CENT COMPANYLIMITED
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