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Method for identifying radix rosae gigantea extract

A technology for the root and extract of G. kung fu, which is applied in the field of identification of G. kung fu root extracts, can solve problems such as difficulty, instability, and incomplete quality control methods

Inactive Publication Date: 2012-05-09
LOGISTICS UNIV OF CAPF
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The incompleteness of its quality control methods and the instability in the production process may cause differences in the quality of each batch of products. It is difficult to say that the purpose of controlling "quality" can be achieved through "quantity"
And if only a single index is used to illustrate its special pharmacological activity, the specificity is poor, but in view of the fact that it is impossible to measure the various components one by one, therefore, a fast and relatively accurate root extract of G. The identification method of its preparations is used to scientifically and effectively identify and control the quality of the traditional Chinese medicine gongguo root extract and its preparations

Method used

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  • Method for identifying radix rosae gigantea extract
  • Method for identifying radix rosae gigantea extract
  • Method for identifying radix rosae gigantea extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: A kind of identification method of the root extract of Gongguo

[0030]1. Instruments and reagents

[0031] 1.1 Instrument

[0032] Shimadzu high performance liquid chromatograph (LC-20AT double pump, SPD-M20AUV detector, CTO-20A column thermostat, Japan Shimadzu Corporation), SHIMADZU VP-ODS column (250×4.60mm, 5micron).

[0033] 1.2 Reagent

[0034] The catechin reference substance was provided by SIGMA Company; the methanol, ethanol, hydrochloric acid, glacial acetic acid, etc. used for extraction were all analytically pure; the methanol and acetonitrile used for liquid phase analysis were all chromatographically pure. Gugu root is collected from Yunnan Province, self-made extract, batch number: 20050918, 20051009, 20061123, 20061128, 20071021, 20071029.

[0035] 2. Methods and Results

[0036] Selection of chromatographic column: Under the same chromatographic conditions, we investigated: Ultimate XB-C18 (150mm×4.6mm, 5μm) column, SHIMADZU VP-ODS c...

Embodiment 2

[0065] A method for identifying the root extract of G. quinquefolius, comprising the steps of:

[0066] The first step is to establish the standard fingerprint of phenolic acid components, the method is as follows:

[0067] (a) Preparation of the test solution: the establishment of the standard fingerprint of the fruit root extract (batch number 20051009)

[0068] The procedure is the same as 3.1 in Example 1, the difference is that in the extraction conditions, add 10 times the volume fraction of the medicinal materials in a methanol solution containing 80% volume fraction, shake, soak and extract for 12 hours, filter, combine the extracts, and depressurize at 60°C Dried to dryness to obtain the root extract of the fruit. Then methanol was dissolved and diluted to configure a 5mg / mL test solution.

[0069] (b) Preparation of the reference substance solution: accurately weigh the reference substance catechin, dissolve and dilute it with methanol as a volume fraction, and con...

Embodiment 3

[0074] A method for identifying the root extract of G. quinquefolius, comprising the steps of:

[0075]The first step is to establish the standard fingerprint of phenolic acid components, the method is as follows:

[0076] (a) Preparation of the test solution: the establishment of the standard fingerprint of the fruit root extract (batch number 20051009)

[0077] Establishment of Standard Fingerprint Spectrum of G. quinquefolium Root Extract (Lot No. 20051009)

[0078] The procedure is the same as 3.1 in Example 1, the difference is that in the extraction conditions, add 10 times the volume fraction of the medicinal materials in a methanol solution containing 80% volume fraction, shake, soak and extract for 12 hours, filter, combine the extracts, and depressurize at 60°C Dried to dryness to obtain the root extract of the fruit. Then dissolve and dilute with ethanol to configure a 20mg / mL test solution.

[0079] (b) Preparation of the reference substance solution: accurately...

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Abstract

The invention discloses a method for identifying a radix rosae gigantea extract, which comprises the following steps of: 1, establishing standard finger-prints of phenolic acid components, namely preparing test solution, preparing reference object solution, preparing octadecyl silica bonded silica gel serving as a stationary phase, performing gradient elution by aqueous solution of acetonitrile and glacial acetic acid, and absorbing the reference object solution and the test solution precisely and respectively, and measuring by liquid chromatography to formulate the finger-prints of the phenolic acid components in the radix rosae gigantea extract, wherein the theoretical plate number is between 5,000 and 20,000 by catechin; 2, measuring the sample finger-prints of the phenolic acid components in the radix rosae gigantea extract to be measured by a method which is the same as that used in the step one; and 3, comparing the sample finger-prints of the phenolic acid components in the radix rosae gigantea extract to be measured with the standard finger-prints of the phenolic acid components in the radix rosae gigantea extract, and identifying the quality of the radix rosae gigantea extract. The invention provides a new method and means for evaluating the quality of the radix rosae gigantea extract completely and accurately.

Description

technical field [0001] The invention relates to a method for identifying the root extract of gongguo. Background technique [0002] The traditional Chinese medicine Gugongguo root is the root of the Rosaceae Rosaceae plant Gugongguo Rose odorata Sweet var. gigantea (Coll.etHemsl.) Rehd.et Wils. It is mild in nature and sour in taste. Kill pathogenic bacteria, protect the mucous membrane of the digestive tract, accelerate the blood circulation of the intestinal wall, and accelerate the healing of the festering intestinal mucosa. It is often used to treat diarrhea, bacillary dysentery and other diseases [1] , is a Yi folk medicinal material with a long history of blind application. At the same time, this medicinal material is also the raw material of the Chinese patent medicine "Changshu Tablet" produced by Yunnan Qujing Pharmaceutical Factory to treat enteritis and diarrhea. Gugongguo is rooted in the folk recipes of the Yi people, and it has a good therapeutic effect on va...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/36A61K36/73A61P1/12A61P31/04A61K125/00
Inventor 刘岱琳马荣朱珊白淑芳陈虹
Owner LOGISTICS UNIV OF CAPF
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