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Methods and use of inducing apoptosis in cancer cells

A cell and purpose technology, applied in the field of inducing cancer cell apoptosis and application, can solve problems such as cell death

Inactive Publication Date: 2013-08-28
BERG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In more than 60% of all cancers, p53 is mutated or inactivated, while the Bcl-2 protein is overexpressed, leading to cell death and resistance to chemotherapeutic approaches

Method used

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  • Methods and use of inducing apoptosis in cancer cells
  • Methods and use of inducing apoptosis in cancer cells
  • Methods and use of inducing apoptosis in cancer cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Protein Expression Protocol (produce image 3 , 4 , 5, 6, 7, 10a-10d, 13, 14, 16, 18, 19a and 25)

[0096] Skmel-28, PC-3 and SkBr3 cells were grown to 80% confluency and subcultured in dishes. After 24 hours, the cells and medium attached to the plate were extracted. Add treatment medium to each plate. After the expected incubation time, the medium was removed and the cells were washed with cold phosphate buffered saline (PBS). Scrape the cells into cold PBS and collect in a centrifuge tube. Cells were then pelleted and washed (3 times) with cold PBS. After removal of PBS, lysis buffer was added and sonicated to disperse protein structures. Add sample buffer to each tube and boil the solution for 5 min. The protein concentration of each sample was quantified using the BCA (bicinchoninic acid) protein assay kit. These values ​​determine the volumes to be added for each sample.

[0097] Samples were loaded onto 4% stacking gel and 12% Tris-Hcl electrophoresis ...

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Abstract

The present disclosure relates to a method of inducing apoptosis in a cancer cell by delivery of exogenous Coenzyme Q1O or its metabolites thereof in a pharmaceutically acceptable carrier to effectuate cell contact of endogenous Coenzyme Q1O or its metabolites thereof in addition to but not limited to mevalonic acid and oleic acid to form an intracellular complex. The present disclosure also provides a method of modulating the p53 pathway and Bcl-2 protein family in a manner that restores the apoptotic potential to a cancer cell by delivery of Coenzyme Q1O in a pharmaceutically acceptable carrier. The present disclosure further provides a method to specifically normalize the ratio of pro-apoptotic and anti-apoptotic members of the Bcl-2 gene family in a proportion to re-program a cancer cell to undergo apoptosis.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of and priority to US Provisional Application 61 / 044,085, filed April 11, 2008, the entire disclosure of which is incorporated herein by reference. Background technique [0003] Programmed cell death (apoptosis) is integral to the maintenance of life, as constant tissue turnover provides physiological support for regenerative metabolism. Apoptosis promotes a homeostatic balance of cell renewal, allowing overall tissue health, thereby maintaining the integrity of the proliferative, immunomodulatory, and angiogenic components of tissue metabolism. Dysregulation of any one or combination of the above processes can lead to a lack of control of apoptosis. This lack of apoptotic control, optionally combined with genetic mutations, may result in a promoting oncogenic environment. [0004] Under healthy conditions, the genome's "guardian" p53 recognizes when the integrity of the cell is com...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/122A61K9/127A61P35/00
CPCA61K9/12A61K9/0048A61K31/122A61K9/0014A61K9/127A61K9/02A61K9/06A61K9/0075A61K9/0019A61P35/00A61P43/00
Inventor N·R·纳莱恩I·佩尔索德J·P·麦克库克
Owner BERG
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