Method for culturing cordyceps militaris sporocarp with high-content bioactive substances

A technology of biologically active substances and cultivation methods, which is applied to the industrial cultivation of Cordyceps militaris fruiting bodies with high content of biologically active substances. Issues such as increasing the yield and content of biologically active substances

Inactive Publication Date: 2011-06-08
陈卫东 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, the invention patent with the application number of 03135295.2 "A method for increasing the content of cordycepin", the invention patent with the application number of 200610117998.9 "the industrial cultivation method of Cordyceps militaris with high content of cordycepin", the invention with the application number of 200810068949.X Patent "Method for increasing cordycepin content in Cordyceps militaris solid medium", application number 01124109.8 Invention patent "Method for producing cordycepin by fermentation culture (liquid culture)", application number 200810068734. Invention patent "Monascus Synergistic Fermentation Method for Improving the Yield of Cordyceps Militaris Fruiting Bodies and Cordycepin", the invention patent application number 200310101650.7 "Application of 3'-deoxyadenosine in the Preparation of Blood Lipid-lowering Drugs", etc. However, these invention patents mainly focus on Cordyceps militaris In the study of the method of improving the yield of fruiting bodies and cordycepin production, there is no research on the increase of the yield of the whole biologically active substance and its content in the fruiting body of Cordyceps militaris

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] In the preparation of the culture medium, the weight share of its various fractions is: choose about 30 grams of rice with a particle size of 12 mesh-20 mesh (accounting for 41% of the total weight), and 30 milliliters of the solution configured in step 2. After calculation, the content of carbon element is 0.47%, and the content of nitrogen is 0.034%. Inject the liquid Cordyceps militaris into the container containing the above-mentioned medium under aseptic conditions. During the vegetative growth period, the ambient temperature of the strain is kept at 12°C±1°C, the humidity is at 65%, and it is protected from light within the first five days. , give scattered light irradiation for the last five days; in the late growth stage, the ambient temperature should be kept at about 20°C, and the relative humidity should be 70%. At the same time, 0.5% glucose solution is sprayed on the surface of the medium to supplement the carbon source. After entering the growth and maturi...

Embodiment 2

[0018]In the preparation of the culture medium, the weight share of its various fractions is: choose about 30 grams of rice with a particle size of 12 mesh-20 mesh (accounting for 41% of the total weight), and 30 milliliters of the solution configured in step 2. After calculation, the content of carbon element is 0.47%, and the content of nitrogen is 0.034%. Inject the liquid Cordyceps militaris into the container containing the above-mentioned medium under aseptic conditions. During the vegetative growth period, the ambient temperature of the strain is kept at 14°C±1°C, the humidity is at 70%, and it is protected from light within the first five days. , give scattered light irradiation for the last five days; in the late growth stage, the ambient temperature should be kept at about 21°C, and the relative humidity should be 75%. At the same time, 0.5% glucose solution is sprayed on the surface of the medium to supplement the carbon source. After entering the growth and maturit...

Embodiment 3

[0020] In the preparation of the culture medium, the weight share of its various fractions is: choose about 30 grams of rice with a particle size of 12 mesh-20 mesh (accounting for 41% of the total weight), and 30 milliliters of the solution configured in step 2. After calculation, the content of carbon element is 0.47%, and the content of nitrogen is 0.034%. Inject the liquid Cordyceps militaris into the container containing the above-mentioned medium under aseptic conditions. During the vegetative growth period, the ambient temperature of the strain is kept at 16°C±1°C, the humidity is at 75%, and it is protected from light within the first five days. , give scattered light irradiation for the last five days; in the late growth stage, the ambient temperature should be kept at about 22°C, and the relative humidity should be 80%. At the same time, 0.5% glucose solution is sprayed on the surface of the medium to supplement the carbon source. After entering the growth and maturi...

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Abstract

The invention provides a method for culturing cordyceps militaris sporocarp with high-content bioactive substances, comprising the following steps: inoculating good cordyceps militaris strains after multiple repeated filtering on a culture medium with the best optimized proportion of carbon to nitrogen sources; and optimizing and culturing at different temperatures, humidity and illumination intensities at different growth periods. Through the cordyceps militaris sporocarp planted and obtained by the method provided by the invention, the contents of bioactive substances are greatly improved, and the bioactive substances mainly comprise the chemical index components of adenosine, cordycepin, cordyceps polysaccharide, cordycepic acid, SOD (Super Oxide Dlsmutase) and the like; for the cordyceps militaris sporocarp planted and obtained by the method provided by the invention, the pharmacology and drug effect are extremely similar to natural Chinese caterpillar fungus, and the contents of bioactive substances of adenosine, cordycepin, cordyceps polysaccharide and cordycepic acid are over that of the natural Chinese caterpillar fungus.

Description

Technical field: [0001] The invention belongs to the technical field of new resource food. In particular, it relates to a method for industrialized cultivation of fruit bodies of Cordyceps militaris with high content of biologically active substances. It particularly relates to an industrialized cultivation method for increasing the content of biologically active substances in fruiting bodies of Cordyceps militaris. Background technique: [0002] Natural wild Cordyceps sinensis is formed in a special ecological and extreme environment. It has special genes and expression products and traits, has strict polytrophy, and requires a special ecological and geographical environment in alpine regions. Therefore, the yield is very small. In 2005, the country banned the digging of wild Cordyceps, so the contradiction between supply and demand is very prominent now. In order to alleviate the contradiction between supply and demand, my country has listed the artificial cultivation of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G1/00
Inventor 陈卫东张华陈春保陈腊梅
Owner 陈卫东
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