Preparation process of fungus-grown loosened green tea
A preparation process, green tea technology, which is applied in the field of preparation of green tea loose tea, can solve the problems of thick and old black hair tea, high fluorine content, and unpopular acceptance, and achieve bright golden color, large particles, and improved The effect of intrinsic quality and added value
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Embodiment 1
[0051] Embodiment 1: Separation and purification of Flos Chinensis:
[0052] The finished sample of Xiangyi brand Fuzhuan brick tea was pulverized with a plant micro pulverizer, sieved with 200 mesh, dried at 65°C for 2 hours, weighed 1g of the sample, fixed to a volume of 10ml, and prepared a concentration of 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 Each 10mL, take 10 -3 、10 -4 、10 -5 Spread 0.0.5mL of each of the three gradients on the PDA plate added with 10% tea juice, blow dry on the ultra-clean table, incubate at 28°C upside down for 3 days, pick the golden yellow dominant colony, and draw a line to the PDA plate added with 10% tea juice On the plate, continue to culture under the same conditions until purification.
[0053] The isolated and purified golden flower colonies are shown in the appendix figure 1 .
Embodiment 2
[0054] Embodiment 2: the spore suspension preparation of golden flower:
[0055] Pick the purified golden flower colony, inoculate it into the PDA liquid medium with 10% tea juice, 20% sucrose, and 10% NaCl, culture it statically at 28°C for 2 days, then raise the temperature to 30-32°C for 7- After 10 days, the disperse conidia were cultured with shaking at 280 rpm, filtered with double-layer gauze, and the OD value measured by a 722-type spectrophotometer was 0.6-0.8, which was the usable golden flower spore suspension.
[0056] The results of the spore suspension culture of Jinhua are attached figure 2 .
Embodiment 3
[0057] Embodiment 3: Verification of green tea loose tea blooming:
[0058] Take respectively 1Kg of two kinds of green teas of different brands on the market, and carry out loose tea fermentation according to steps 2-8 in the specific embodiment.
[0059] Green tea loose tea flowering results see attached image 3 , Figure 4 .
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