Adaption method of influenza virus vaccine strains on Vero cells

Abstract

The invention provides an adaption method of influenza virus vaccine strains on Vero cells. The influenza virus vaccine strains are rapidly adapted on the Vero cells; and various effective virus adaption methods are established, so that influenza virus seeds can be adapted on the Vero cells, and virus progenies used as vaccines are produced under the condition of minimizing virus surface antigen variation caused by virus adaptation selection. The hemagglutination titer of the virus seeds prepared by vaccine strains adapted by the method is more than or equal to 160, and meets the requirementsof three parts of the Chinese pharmacopoeia, 2010 edition.

Description

technical field

[0001] The invention relates to a method for adapting multiple influenza virus vaccine strains on Vero cells, and obtains vaccine-adapted strains and virus seed batches to prepare Vero cell influenza vaccines, belonging to the field of biotechnology. Background technique

[0002] The traditional method of preparing influenza vaccine is to use chicken embryos, which have been used in China for more than 50 years to cultivate vaccines. The currently used influenza vaccines are mainly inactivated influenza trivalent split vaccines prepared from chicken embryos, including influenza A strains, H 3 N 2 and influenza B strains. There are also influenza vaccines (including H 5 N 1 wait). The source of the vaccine strain is mainly determined by the surface antigen of the influenza virus epidemic strain recommended by the WHO according to the annual change of the global influenza virus for the next year's influenza vaccine production. strain (A / PR / 8 / 34 (H 1 N 1...