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Application of cystic fibrosis transmembrane transduction regulating factor inhibitor to preparation of medicaments for treating diabetes

A cystic fibrosis and diabetes drug technology, applied in the field of cystic fibrosis transmembrane transduction regulator inhibitors, to achieve the effect of promoting insulin secretion and stimulating release

Inactive Publication Date: 2011-07-27
BEIJING TONGREN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, CFTR inhibitors are used as insulin secretion accelerators, and there are no reports on the drugs used in the treatment of diabetes.

Method used

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  • Application of cystic fibrosis transmembrane transduction regulating factor inhibitor to preparation of medicaments for treating diabetes
  • Application of cystic fibrosis transmembrane transduction regulating factor inhibitor to preparation of medicaments for treating diabetes
  • Application of cystic fibrosis transmembrane transduction regulating factor inhibitor to preparation of medicaments for treating diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Cell culture:

[0042] The MIN-6 islet β cell line was cultured in Hyclone DMEM (glucose concentration 5.6mM) medium, supplemented with 10% fetal bovine serum (v / v), 100U / mL penicillin, 100μg / mL streptomycin, at 37°C, Contains 5% CO 2 , 95% air incubator. Change the medium every 2-3 days, and when the confluence rate of the cells at the bottom of the bottle reaches about 80%, subculture at 1:2.

[0043] (2) Main reagents and instruments:

[0044] DMEM medium (Hyclone), fetal bovine serum (Sigma), CFTRinh-172 (Sigma), Rat Insulin ELISA Kit kit.

[0045] (3) The effect of CFTRinh-172 on the secretion of insulin by pancreatic beta cells:

[0046] Take MIN-6 cells in logarithmic growth phase, inoculate 2*105 cells per well in 24-well plates, and divide them into 0in group (6.25mM bicarbonate in extracellular fluid, without CFTR blocker CFTRinh-172) , 0.02in group (6.25mM bicarbonate in extracellular fluid, 0.02μmol / L CFTRinh-172), 0.1in group (6.25mM bicarbonate in...

Embodiment 2

[0049] (1) Cell culture:

[0050] Cell culture was performed in the same manner as in Example 1.

[0051] (2) Main reagents and instruments:

[0052] DMEM medium (Hyclone Company), fetal bovine serum (Sigma), MTT (Sigma Company, USA), PCR primers, analytical alcohol and RNA extraction kit (Shangbai Biomedical Co., Ltd.), PCR amplification instrument and microplate reader, CFTR antibody[CF3](Abcam), Cy5-labeled goat anti-mouse IgG(H+L)(BYT), Txiton X-100(Sigma), DAB kit(BYT), CFTR(inh)-172(Sigma) , Rat InsulinELISA Kit kit.

[0053] (3) Grouping:

[0054] MIN-6 cells were divided into six groups, 0SB group (without extracellular bicarbonate), 6.25SB group (the concentration of extracellular fluid bicarbonate was 6.25mM), 12.5SB group (the concentration of extracellular fluid bicarbonate is 12.5mM), 25SB group (the concentration of bicarbonate in extracellular fluid is 25mM).

[0055] (4) Regulation of extracellular bicarbonate concentration on insulin secretion from pancre...

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PUM

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Abstract

The invention relates to application of cystic fibrosis transmembrane transduction regulating factor inhibitor to the preparation of medicaments for treating diabetes, and application of thiazolidone compounds or derivatives thereof to the preparation of medicaments for treating diabetes. The cystic fibrosis transmembrane transduction regulating factor inhibitor can be subjected to compatibility with medicaments of sulfonylurea, glinides, biguanides, insulin sensitizers and glycosidase inhibitors.

Description

technical field [0001] The invention relates to the application of an inhibitor of cystic fibrosis transmembrane transduction regulator in medicine for treating diabetes. Background technique [0002] Cystic fibrosis transmembrane conductance regulator (cystic fibrosis transmembrane conductance regulator, CFTR) is a special member of the ATP binding cassette transporter superfamily. CFTR consists of 5 domains: 2 membrane-spanning domains (MSDs), 2 nucleotide-binding domains (NBDs), and 1 special regulatory domain (RD) ),Such as figure 1 shown. The CF gene is located at 7q31, with a total length of about 250kbp and a total of 27 exons. The cDNA is about 6129bp in length and encodes a peptide chain with a length of 1480 amino acids. The latter is named CFTR (Riordan JR, Rommens JM, Kerem B, et al . Identification of the systic fibrosis gene: cloning and characterization of complementary DNA [J]. Science, 1989, 245 (4922): 1066-1073). In 1991, CFTR was identified as a chlor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K45/06A61K31/426A61P3/10
Inventor 杨金奎吕晓红王蕾于湄
Owner BEIJING TONGREN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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