Pollen pini water extract for treating child sexual precocity, preparation method and use of pollen pini water extract
A technology of water extract and pine pollen, which is applied in the direction of medical raw materials derived from gymnosperms, drug combinations, endocrine system diseases, etc., to achieve the effect of low price, convenient use and rapid absorption
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[0031] 1. Prepare the aqueous extract of gymnosperm pine pollen for treating precocious puberty in children:
[0032] 1. Sorting pine pollen: remove pine pollen impurities at room temperature.
[0033] 2. Wall-breaking treatment: the impurity-removed pine pollen is subjected to air-flow crushing and wall-breaking treatment for 10 to 20 minutes.
[0034] 3. Soaking and ultrasonic treatment: Soak the pine pollen after wall-breaking treatment with normal pressure and normal temperature water for 10-15 minutes, and then ultrasonic treatment for 20-40 minutes.
[0035] 4. Centrifuge to remove solid impurities, take the aqueous solution: centrifuge for 10-15 minutes in a refrigerated centrifuge with a rotating speed of 8000-10000 rpm, and take the supernatant.
[0036] 5. Ultrafiltration: Use 4000D molecular sieve ultrafiltration on the supernatant taken at an ambient temperature of 1-5°C to separate the pine pollen water extract with a molecular weight below 4000D, which is used t...
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