Method for extracting grape seed oil containing oligomeric proantho cyanidins (OPC)

A grape seed oil and proanthocyanidin technology, which is applied in the direction of fat oil/fat production and fat production, can solve the problems of solvent residue, unstable product quality, low yield, etc., and achieve the effect of improving antioxidant activity

Inactive Publication Date: 2011-08-10
BEIFANG UNIV OF NATITIES
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Problems solved by technology

Among them, the pressing method has more product loss and low yield; although the solvent extraction method can o

Method used

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Effect test

Embodiment 1

[0014] A method for extracting grape seed oil containing proanthocyanidins, comprising the steps of: a, crushing grape seeds by plant tissue crushing extraction method, adding ethanol with a weight ratio of 0.5 times of grape seeds, plant tissue crushing extractor rotating speed is 1500r / min, extracting Time 0.5min; b, the crushed grape seeds with supercritical CO 2 Extraction, CO 2 The flow rate is 1L / h, the extraction pressure is 10MPa, the temperature is 40°C, the pressure in the primary separation kettle is 5MPa, the pressure in the secondary separation kettle is normal pressure, the extraction time is 30min, and the grape seed oil containing proanthocyanidins is obtained; c. The grape seed oil containing proanthocyanidins obtained in the previous step was allowed to stand at room temperature for 8 hours, and then the ethanol was separated.

Embodiment 2

[0016] A method for extracting grape seed oil containing proanthocyanidins, comprising the steps of: a, crushing grape seeds by a plant tissue crushing extraction method, adding an aqueous ethanol solution with a water content of 40% V / V that is 2 times the weight ratio of grape seeds, and crushing the plant tissue The speed of the extractor is 6000r / min, and the extraction time is 4min; 2 Extraction, CO 2 The flow rate is 15L / h, the extraction pressure is 30MPa, the temperature is 60°C, the pressure in the primary separation kettle is 15MPa, the pressure in the secondary separation kettle is normal pressure, the extraction time is 180min, and the grape seed oil containing proanthocyanidins is obtained; c. The grape seed oil containing proanthocyanidins obtained in the previous step was left to stand at room temperature for 24 hours, and the ethanol was separated.

Embodiment 3

[0018] A method for extracting grape seed oil containing proanthocyanidins, comprising the following steps: a, crushing grape seeds by a plant tissue crushing extraction method, adding an aqueous ethanol solution with a water content of 10% V / V of 1 times the weight ratio of grape seeds, and crushing the plant tissue The speed of the extractor is 5000r / min, and the extraction time is 2.5min; b, the crushed grape seeds are treated with supercritical CO 2 Extraction, CO 2 The flow rate is 10L / h, the extraction pressure is 20MPa, the temperature is 45°C, the pressure in the primary separation kettle is 10MPa, the pressure in the secondary separation kettle is normal pressure, the extraction time is 120min, and the grape seed oil containing proanthocyanidins is obtained; c. The grape seed oil containing proanthocyanidins obtained in the previous step was allowed to stand at room temperature for 12 hours, and then the ethanol was separated.

[0019] The grapeseed oil extracted by ...

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Abstract

The invention relates to a method for extracting grape seed oil from grape seeds, in particular to a method for extracting grape seed oil containing oligomeric proantho cyanidins (OPC), which is characterized by comprising the following steps: a, crushing grape seeds by using a plant tissue crushing extraction method; b, carrying out supercritical CO2 leaching on the crushed grape seeds to obtainthe grape seed oil containing OPCs; and c, standing to separate out ethanol. By using the extraction process provided by the invention, the extraction rate of the grape seed oil can be up to 98.72%, the content of OPCs in the oil is 2.14mg/mL, and the scavenging rate IC50 value of diphenylpicrylhydrazyl (DPPH) free radicals is 11.45mg/mL and is lower than the IC50 value (12.80mg/mL) of the grape seed oil not containing OPCs, thereby proving that the antioxidant activity is improved.

Description

technical field [0001] The invention relates to a method for extracting grape seed oil from grape seeds, in particular to a method for extracting proanthocyanidin-containing grape seed oil. Background technique [0002] Grape seeds are mainly derived from the leftovers of the grape wine industry, accounting for 5%-7% of the whole grape, and grape seeds contain 13%-17% of oil. According to reports, the content of unsaturated fatty acids in grape seed oil is as high as 90%, and the content of linoleic acid is as high as 80%. Proanthocyanidins are polymerized by different numbers of catechins. They have good antioxidant activity and can prevent, delay and inhibit aging damage caused by excessive free radicals. , Anti-gene mutation, anti-radiation, repair collagen, prevent melanin formation and whitening, etc. have broad development and application prospects. [0003] For a long time, the extraction of grape seed oil has mostly been done by pressing or solvent extraction. Amo...

Claims

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Application Information

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IPC IPC(8): C11B1/04C11B1/10
Inventor 杨晋王吉壮赵鹏姜国平王皓
Owner BEIFANG UNIV OF NATITIES
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