Vertical multi-layer closed nuclear polyhedrosis virus multiplication process

Abstract

The invention discloses a vertical multi-layer closed nuclear polyhedrosis virus multiplication process, which is accomplished by the following steps: 1, selecting a land, which has an area of more than or equal to 50m<2> and has naturally or artificially growth plant feed around and a water source nearby, as a multiplication room; 2, building a vertical trapezoid platform in the multiplication room for breeding larvae; 3, closing the doors and windows of the multiplication room; 4, collecting 2-2.5-year-old larvae of hyphantria cunea and placing the larvae on the multiplication platform flatly; 5, spraying viral liquid onto the placed larvae of hyphantria cunea; and 6, collecting corpses of larvae dead of diseases in a container containing sterile water to soak the corpses for 2 to 4 days, grinding and crushing the corpses in a homogenizing crusher to obtain homogenate, filtering the homogenate with a gauze element, subjecting residue filtered out to secondary grinding, filtering with the gauze element, and obtaining hyphantria cunea polyhedrosis virus liquid. The process technique is simple, can improve the number of multiplied viruses, reduce the cost of virus multiplication, facilities the collection of dead larvae infected with the virus and is suitable to be promoted and used in vast rural areas.

Description

Technical field: [0001] The invention belongs to a virus propagation process, in particular to a three-dimensional multi-layer closed nuclear polyhedrosis virus propagation process. Background technique: [0002] Since the American white moth was discovered in Liaoning Province in 1979, the epidemic has spread in Tianjin, Beijing, Hebei and other provinces and cities. Jixian County, Tianjin is a key area where white moths occur in the United States. The annual occurrence area is more than 40,000 hectares, and more than 10 million yuan is invested in prevention and control each year. White moth larvae can reproduce for 2-3 generations in the north every year. It has the characteristics of large amount, wide transmission route and serious harm, which will cause direct harm to forest fruits, crops and green plants. [0003] American white moth nuclear polyhedrosis virus is a natural pathogen of American white moth and the nemesis of American white moth larvae. The lethal rate ...

AI Technical Summary

Problems solved by technology

The disadvantage is that it needs to purchase related equipment, feed, technical difficulty and high cost, so it is not suitable for rural application