Preparation method of multiplex-mode iodized oil nano-emulsion contrast medium

A technology of contrast agent and nanoemulsion, which is applied in MRI/MRI contrast agent, emulsion delivery, drug delivery, etc., can solve the problems of incomplete and inaccurate detection information of complex diseases.

Inactive Publication Date: 2011-08-31
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008]Technical problem: the purpose of this invention is to address the shortcomings of CT, MRI, and fluorescence imaging techniques when they are used alone, resulting in incomplete and inaccurate detection information for complex diseases. , proposed a method for preparing a multimodal lipiodol nanoemulsion contrast agent, and the prepared multimodal lipiodol nanoemulsion contrast agent can be used for CT / MRI dual-modal contrast or CT / fluorescence dual-mode imaging on patients through intravenous injection. It can also be used for CT / MRI / fluorescence three-modal contrast in order to achieve comprehensive and accurate diagnosis of diseases, thereby further promoting the development of clinical medicine and imaging diagnostics

Method used

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  • Preparation method of multiplex-mode iodized oil nano-emulsion contrast medium
  • Preparation method of multiplex-mode iodized oil nano-emulsion contrast medium
  • Preparation method of multiplex-mode iodized oil nano-emulsion contrast medium

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Experimental program
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Effect test

Embodiment 1

[0039] Embodiment one, Iodized oil injection was selected as part of the CT contrast agent, the oil-soluble Fe 3 o 4 Nanoparticles used as part of MRI contrast agent to prepare lipiodol-encapsulated oil-soluble Fe for CT / MRI dual-modal contrast 3 o 4 Method of nanoparticle oil-in-water nanoemulsion:

[0040] 1) Take oil-soluble Fe 3 o 4 Nanoparticles 31.9mg, add 500μl iodized oil injection, vortex

[0041] The instrument was shaken for 5 minutes to make the mixture uniform, and then ultrasonic cleaner was used for 10 minutes to fully mix, and the obtained mixture was used as the oil phase.

[0042] 2) Take 100mg Lipoid E-80, add 200μl absolute ethanol, dissolve it by ultrasonic; weigh 180mg poly

[0043] Ethylene glycol (25) monostearate was dissolved in a three-necked bottle filled with 20ml of ultrapure water, which was used as the aqueous phase.

[0044] 3) Put the three-necked bottle on the electric stove and heat until the water phase boils, then add the dissolve...

Embodiment 2

[0048] Embodiment two, Lipiodol injection was selected as the CT contrast agent part, and oil-soluble CdSe / ZnS quantum dots were used as the optical probe part to prepare lipiodol-encapsulated oil-soluble CdSe / ZnS quantum dots for CT / fluorescence dual-modal imaging The method of oil-in-water nanoemulsion:

[0049] 1) Take 5.5 mg of oil-soluble CdSe / ZnS quantum dots, add 500 μl iodized oil injection, and vortex

[0050] The instrument was shaken for 5 minutes to make the mixture uniform, and then ultrasonic cleaner was used for 10 minutes to fully mix, and this mixture was used as the oil phase.

[0051] 2) Take 100mg Lipoid E-80, add 200μl absolute ethanol, dissolve by ultrasonication for 5min; weigh 180mg

[0052] Polyethylene glycol (25) monostearate was dissolved in a three-necked bottle filled with 20ml of ultrapure water, which was used as the aqueous phase.

[0053] 3) Put the three-necked bottle on the electric stove and heat until the water phase boils, then add th...

Embodiment 3

[0057] Embodiment three, Iodized oil injection was selected as part of the CT contrast agent, the oil-soluble Fe 3 o 4 Nanoparticles are used as the MRI contrast agent part, and oil-soluble CdSe / ZnS quantum dots are used as the optical probe part to prepare lipiodol for CT / MRI / fluorescence three-mode imaging while encapsulating oil-soluble Fe3O4 nanoparticles and oil-soluble CdSe The method of the oil-in-water nanoemulsion of / ZnS quantum dot:

[0058] 1) Take 5.5mg of oil-soluble CdSe / ZnS quantum dots, take oil-soluble Fe 3 o 4 Nanoparticles 31.9mg, add 500

[0059] μl iodized oil injection, vortex for 5 minutes to make the mixture evenly, and then ultrasonic cleaner

[0060] 10min until evenly dispersed. This mixture serves as the oil phase.

[0061] 2) Take 100mg of Lipoid E-80, add 200μl of absolute ethanol, and ultrasonically dissolve; weigh 180mg of polyethylene glycol (25) monostearate, and dissolve it in a three-necked bottle filled with 20ml of ultrapure water...

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Abstract

The invention discloses a preparation method of multiplex-mode iodized oil nano-emulsion contrast medium. Aiming at the problems of incomplete detection information of complex diseases and the like because of the shortages when CT (Computed Tomography), MRI (Magnetic Resonance Imaging) and the fluorescence imaging technology are independently used, the invention provides a preparation method of multiplex-mode iodized oil nano-emulsion contrast medium. The method is characterized in that an oil-soluble Fe3O4 nano particles are chosen as an MRI contrast medium part and an oil-soluble CdSe / ZnS quantum dots are chosen as an optical probe part on the basis that iodized oil injection is chosen as a CT contrast medium. Firstly, the oil-soluble Fe3O4 nano particles and / or oil-soluble CdSe / ZnS quantum dots are fully dissolved into the iodized oil injection to serve as an oil phase; the oil phase is added into an aqueous phase containing joint surfactant; stable oil-in-water nano-emulsion is formed with a stirring and ultrasonic emulsification method; and finally, the transparent and clear multiplex-mode iodized oil nano-emulsion contrast medium with proper size, good homogeneity, good stability and high biocompatibility is obtained.

Description

technical field [0001] The invention provides a multimodal lipiodol nanoemulsion contrast agent for imaging diagnosis and a preparation method thereof. It belongs to the field of biological and medical nanomaterials and technology. Background technique [0002] Multimodal contrast agent refers to a contrast agent that can perform combined imaging of two or more imaging devices such as CT, MRI, and fluorescence at the same time by injecting one contrast agent at a time. At present, CT and MRI imaging technologies are the two most common imaging diagnostic methods, while optical imaging technology has received more and more attention due to its own advantages. CT contrast agents commonly used in clinical practice are usually derivatives of triiodobenzene ring. Superparamagnetic Fe3O4 nanoparticles have outstanding advantages as an MRI contrast agent and have been applied clinically. Optical imaging reagents mainly include fluorescent dyes and quantum dots. However, in mode...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/18A61K49/06
Inventor 张宇王月花丁佳丽马明顾宁
Owner SOUTHEAST UNIV
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