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Single-tube multi-primer mini-pool (MP) HIV (human immunodeficiency virus) nucleic acid test kit

A technology with multiple primers and kits, which is applied in the determination/testing of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of unsuitable popularization and high cost

Inactive Publication Date: 2011-08-31
BEIJING YOUAN HOSPITAL CAPITAL MEDICAL UNIV
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Problems solved by technology

Pan Pinliang et al. established a COBAS Amplicor-based RT-PCR method for detecting "window period" HIV-infected persons, which can reach 50 people as a detection set, but this method is more expensive than ordinary PCR and requires specific equipment , should not be popularized

Method used

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  • Single-tube multi-primer mini-pool (MP) HIV (human immunodeficiency virus) nucleic acid test kit
  • Single-tube multi-primer mini-pool (MP) HIV (human immunodeficiency virus) nucleic acid test kit
  • Single-tube multi-primer mini-pool (MP) HIV (human immunodeficiency virus) nucleic acid test kit

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Embodiment Construction

[0027] 1. Source: Specimens used to establish the method: 30 HIV antibody-positive and known HIV subtypes and viral load samples from a cohort of MSM-infected individuals (CRF01-AE 15 cases, B subtype 10 cases, CRF07- 4 cases of BC; 1 case of CRF15-01B, related articles on subtype identification have been published in other papers [5,6] ), 3 samples from patients in the window period confirmed by commercial nucleic acid detection methods (Easy Q and bDNA) and 97 HIV-negative samples from normal people were all from plasma stored at -80°C in Beijing You'an Hospital; 1005 samples to be tested came from Beijing HIV antibody-negative or indeterminate plasma collected from the MSM cohort and a small number of other high-risk groups collected by You'an Hospital. The high-risk groups are all male, aged 18-56 years.

[0028] 2. Experimental reagents and primer design The one-tube viral RNAout extraction kit was purchased from Beijing Tianenze Gene Technology Co., Ltd., and the one-s...

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Abstract

The invention establishes a single-tube multi-primer mini-pool (MP) human immunodeficiency virus (HIV) nucleic acid test (NAT) technology with combination of RNA (ribonucleic acid) reverse transcription-polymerase chain reaction (RT-PCR) and nested PCR, which is applied in men who have sex with men (MSM) and other high-risk groups for detection during the window period.

Description

[0001] technical field [0002] The invention relates to a detection kit, in particular to a single-tube multi-primer small collection HIV nucleic acid detection kit. Background technique [0003] Acutely infected persons after HIV infection before the emergence of antibodies have a high viral load in the blood and are unaware of their infection status. The risk of HIV transmission is much higher than that of chronically infected persons. Therefore, early detection of these infected persons is very important. public health significance. At present, the AIDS epidemic in my country is spreading from high-risk groups to the general population, and sexual transmission has become the main way of AIDS transmission. Gay men and women (MSM) are a high-risk group of HIV infection, and early detection of infected persons among them can achieve twice the result with half the effort. The early detection of these groups mainly relies on the detection of viral antigens and nucleic acid ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12N15/11
Inventor 刘志英李岚张彤魏飞力焦艳梅石英陈德喜吴昊
Owner BEIJING YOUAN HOSPITAL CAPITAL MEDICAL UNIV
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