Cell cryopreserving liquid as well as preparation method and application thereof

A cryopreservation, cell technology, applied in applications, animal cells, preservation of human or animal bodies, etc., can solve the problem of low survival rate, improve the survival rate, ensure quality and biological characteristics, and shorten the proliferation cycle. Effect

Active Publication Date: 2011-09-07
BEIJING MINHAI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] A large number of actual biological cytology experiments and a large number of statistical data show that the survival rate of cells frozen in this freezing solution is generally 50-70% after recovery, and the survival rate is relatively low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Weigh 3g of glutamine and dissolve it in an appropriate amount of water, set the volume to 100ml, and prepare a 3% (w / v) glutamine solution. Weigh 7.5g of sodium bicarbonate, dissolve it in an appropriate amount of water, and set the volume to 100ml to prepare a 7.5% (w / v) sodium bicarbonate solution.

[0036] Measure 10ml of MEM cell culture fluid, 75ml of calf serum, 10ml of dimethylsulfoxide, 2ml of 3% (w / v) glutamine solution and 3ml of 7.5% (w / v) bicarbonate Sodium solution, mixed to prepare the cell cryopreservation solution of the present invention.

Embodiment 2

[0038] The difference between this embodiment and embodiment 1 is:

[0039] Measure 15ml of M199 cell culture fluid, 70ml of calf serum, 10ml of dimethylsulfoxide, 2ml of 3% (w / v) glutamine solution and 3ml of 7.5% (w / v) sodium bicarbonate solution, mixed to prepare the cell freezing solution of the present invention.

Embodiment 3

[0041] The difference between this embodiment and embodiment 1 is:

[0042] Take 5ml of Eagle cell culture medium, 80ml of calf serum, 10ml of dimethylsulfoxide, 2ml of 3% (w / v) glutamine solution and 3ml of 7.5% (w / v) sodium bicarbonate solution, mixed to prepare the cell freezing solution of the present invention.

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PUM

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Abstract

The invention provides a cell cryopreserving liquid as well as a preparation method and application thereof. The cell cryopreserving liquid comprises 5-20% (v / v) of cell culture solution, 65-80% (v / v) of calf serum, 10% (v / v) of dimethyl sulfoxide, 1-3% (v / v) of 3% (w / v) glutamine solution and 2-4% (v / v) of 7.5% (w / v) sodium bicarbonate solution. The cell freezing preserving liquid provided by the invention is used for culturing the cell in a cryopreserving way, thus the survival rate of the cryopreserved cells after being recovered is increased, the cell growing quality is assured and the cell proliferation time is reduced.

Description

technical field [0001] The invention relates to the technical field of tissue cell culture, in particular to a cell cryopreservation solution and its preparation method and application. Background technique [0002] Cell cryopreservation solution is a necessary solution for cell cryopreservation. For a long time, the conventional cryopreservation solution formula introduced by a large number of domestic and foreign biological cell academic books and the volume percentage of each component of the cryopreservation solution we have used for a long time are: cell Culture medium 70-80%, bovine serum 10-30%, dimethyl sulfoxide 10%. Conventionally used culture media such as MEM, M199, Eagle and DMEM have been commercialized in the market. [0003] The function of the cryopreservation solution is to disperse the cells evenly in the solution, give the cells an evenly distributed environment and space, and at the same time supply the nutrients necessary for the metabolism of the cell...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02C12N5/07
Inventor 徐希丽林登宇
Owner BEIJING MINHAI BIOTECH
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